排序方式: 共有107条查询结果,搜索用时 15 毫秒
41.
Chaurand P Latham JC Lane KB Mobley JA Polosukhin VV Wirth PS Nanney LB Caprioli RM 《Journal of proteome research》2008,7(8):3543-3555
Imaging mass spectrometry is becoming a key technology for the investigation of the molecular content of biological tissue sections in direct correlation with the underlying histology. Much of our work has been done with fresh-frozen tissue sections that has undergone minimal protein degradation between the time a tissue biopsy is sampled and the time it is snap-frozen so that no preserving or fixing agents need to be added to the frozen biopsy. However, in many sampling environments, immediate flash freezing may not be possible and so we have explored the use of ethanol-preserved, paraffin-embedded tissue specimens for proteomic analyses. Solvent-only preserved tissue specimens provide long-term preservation at room temperature, generation of high quality histological sections and little if any chemical alteration of the proteins. Using mouse organs, several key steps involved in the tissue dehydration process have been investigated to assess the potential of such preserved specimens for profiling and imaging mass spectrometry investigations. 相似文献
42.
Zhirekhina OV Chukhlovin AB Sysoev KA Gritsenko VV Totolian AA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2008,(4):96-98
Infectious endocarditis can be caused by various microorganisms. Diagnostics of local infection by microbiological methods is not always effective. For that reason we performed a study aimed for direct detection of potential infectious agents by polymerase chain reaction in patients' heart valve tissue. DNA of infectious agents was revealed in 72% of heart valve tissue samples from patients with septic endocarditis; in studied samples, along with bacterial DNA, herpesviruses' DNA was detected. Obtained results confirm the presence of infection, which allows to perform specific diagnostics of infectious complications after implantation of prosthetic cardiac valves. 相似文献
43.
Alymova IV Taylor G Mishin VP Watanabe M Murti KG Boyd K Chand P Babu YS Portner A 《Journal of virology》2008,82(17):8400-8410
BCX 2798 (4-azido-5-isobutyrylamino-2,3-didehydro-2,3,4,5-tetradeoxy-d-glycero-d-galacto-2-nonulopyranosic acid) effectively inhibited the activities of the hemagglutinin-neuraminidase (HN) of human parainfluenza viruses (hPIV) in vitro and protected mice from lethal infection with a recombinant Sendai virus whose HN was replaced with that of hPIV-1 (rSeV[hPIV-1HN]) (I. V. Alymova, G. Taylor, T. Takimoto, T. H. Lin., P. Chand, Y. S. Babu, C. Li, X. Xiong, and A. Portner, Antimicrob. Agents Chemother. 48:1495-1502, 2004). The ability of BCX 2798 to select drug-resistant variants in vivo was examined. A variant with an Asn-to-Ser mutation at residue 173 (N173S) in HN was recovered from mice after a second passage of rSeV(hPIV-1HN) in the presence of BCX 2798 (10 mg/kg of body weight daily). The N173S mutant remained sensitive to BCX 2798 in neuraminidase inhibition assays but was more than 10,000-fold less sensitive to the compound in hemagglutination inhibition tests than rSeV(hPIV-1HN). Its susceptibility to BCX 2798 in plaque reduction assays was reduced fivefold and did not differ from that of rSeV(hPIV-1HN) in mice. The N173S mutant failed to be efficiently eluted from erythrocytes and released from cells. It demonstrated reduced growth in cell culture and superior growth in mice. The results for gel electrophoresis analysis were consistent with the loss of the N-linked glycan at residue 173 in the mutant. Sequence and structural comparisons revealed that residue 173 on hPIV-1 HN is located close to the region of the second receptor-binding site identified in Newcastle disease virus HN. Our study suggests that the N-linked glycan at residue 173 masks a second receptor-binding site on hPIV-1 HN. 相似文献
44.
The DNA polymerases (gp43s) of the two related phages T4 and RB69 are DNA-binding proteins that also function as mRNA-binding autogenous translational repressors. As repressors, T4 gp43 is narrowly specific to its own mRNA whereas RB69 gp43 is equally effective against mRNA for either protein. We used in vitro RNase-sensitivity and RNA footprinting assays to identify features of the non-identical T4 and RB69 mRNA targets (translational operators) that allow for their identical binding affinities and biological responses to RB69 gp43. We observed that T4 gp43 and RB69 gp43 produce identical footprints on RNA substrates bearing the T4-derived operator, suggesting that the two gp43s make identical contacts with this operator. In contrast, the footprint produced by RB69 gp43 on its autogenous RNA target was shorter than its footprint on operator RNA from T4. As expected, we also observed only weak protection of RB69-derived operator RNA from RNase by T4 gp43; however, photocross-linking studies suggested that T4 gp43 recognizes structural features of the RB69-derived operator that are not detected by RNase- sensitivity assays. The results suggest that RB69 gp43 and T4 gp43 differ in their abilities to use RNA-sequence-independent interactions to configure potential RNA targets for translational repression. 相似文献
45.
Sysoev Yu. I. Shits D. D. Puchik M. M. Prikhodko V. A. Idiyatullin R. D. Kotelnikova A. A. Okovityi S. V. 《Journal of Evolutionary Biochemistry and Physiology》2022,58(4):1130-1141
Journal of Evolutionary Biochemistry and Physiology - Research and development of novel methods to determine the effects of antipsychotic agents is an important challenge for experimental... 相似文献
46.
Artem’ev K. V. Batanov G. M. Berezhetskaya N. K. Borzosekov V. D. Davydov A. M. Kozhevnikova N. A. Konchekov E. M. Kossyi I. A. Sarksyan K. A. Stepakhin V. D. Sysoev S. O. Temchin S. M. Kharchev N. K. 《Plasma Physics Reports》2019,45(6):523-526
Plasma Physics Reports - A subthreshold microwave discharge in atmospheric-pressure air is used to process mixtures of mercaptans (thiols) with air and with air and methane. It is found that, at... 相似文献
47.
The subsystems approach to genome annotation and its use in the project to annotate 1000 genomes 总被引:12,自引:1,他引:12
Overbeek R Begley T Butler RM Choudhuri JV Chuang HY Cohoon M de Crécy-Lagard V Diaz N Disz T Edwards R Fonstein M Frank ED Gerdes S Glass EM Goesmann A Hanson A Iwata-Reuyl D Jensen R Jamshidi N Krause L Kubal M Larsen N Linke B McHardy AC Meyer F Neuweger H Olsen G Olson R Osterman A Portnoy V Pusch GD Rodionov DA Rückert C Steiner J Stevens R Thiele I Vassieva O Ye Y Zagnitko O Vonstein V 《Nucleic acids research》2005,33(17):5691-5702
The release of the 1000th complete microbial genome will occur in the next two to three years. In anticipation of this milestone, the Fellowship for Interpretation of Genomes (FIG) launched the Project to Annotate 1000 Genomes. The project is built around the principle that the key to improved accuracy in high-throughput annotation technology is to have experts annotate single subsystems over the complete collection of genomes, rather than having an annotation expert attempt to annotate all of the genes in a single genome. Using the subsystems approach, all of the genes implementing the subsystem are analyzed by an expert in that subsystem. An annotation environment was created where populated subsystems are curated and projected to new genomes. A portable notion of a populated subsystem was defined, and tools developed for exchanging and curating these objects. Tools were also developed to resolve conflicts between populated subsystems. The SEED is the first annotation environment that supports this model of annotation. Here, we describe the subsystem approach, and offer the first release of our growing library of populated subsystems. The initial release of data includes 180177 distinct proteins with 2133 distinct functional roles. This data comes from 173 subsystems and 383 different organisms. 相似文献
48.
49.
A numerical model of a spark discharge propagating along the ground surface from the point at which an ∼100-kA current pulse
is input into the ground has been developed based on experiments in which the velocity of a long leader was measured as a
function of the leader current. The results of numerical simulations are in good agreement with the measured characteristics
of creeping discharges excited in field experiments by using a high-power explosive magnetic generator. The reason why the
length of a spark discharge depends weakly on the number of simultaneously developing channels is found. Analysis of the influence
of the temporal characteristics of the current pulse on the parameters of the creeping spark discharge shows that actual lighting
may exhibit similar behavior. 相似文献
50.
Elizaveta V. Demidova Tatiana N. Goryachkovskaya Tatiana K. Malup Svetlana V. Bannikova Artem I. Semenov Nikolay A. Vinokurov Nikolay A. Kolchanov Vasiliy M. Popik Sergey E. Peltek 《Bioelectromagnetics》2013,34(1):15-21
Studies of the impact of terahertz radiation on living objects present a significant interest since its use for security systems is currently considered promising. We studied the non‐thermal impact of terahertz radiation on E. coli/pKatG‐gfp biosensor cells. The Novosibirsk free electron laser (NovoFEL), which currently has the world's highest average and peak power, was used as the source of terahertz radiation. We demonstrated that exposure to terahertz radiation at the wavelengths of 130, 150, and 200 µm and a power of 1.4 W/cm2 induces changes in green fluorescent protein (GFP) fluorescence values and thus induces the expression of GFP in E. coli/pKatG‐gfp biosensor cells. Possible mechanisms of the E. coli response to non‐thermal exposure to terahertz radiation are discussed. Bioelectromagnetics 34:15–21, 2013. © 2012 Wiley Periodicals, Inc. 相似文献