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41.
The anti-apoptotic protein HAX-1 interacts with SERCA2 and regulates its protein levels to promote cell survival 总被引:1,自引:0,他引:1
Vafiadaki E Arvanitis DA Pagakis SN Papalouka V Sanoudou D Kontrogianni-Konstantopoulos A Kranias EG 《Molecular biology of the cell》2009,20(1):306-318
Cardiac contractility is regulated through the activity of various key Ca2+-handling proteins. The sarco(endo)plasmic reticulum (SR) Ca2+ transport ATPase (SERCA2a) and its inhibitor phospholamban (PLN) control the uptake of Ca2+ by SR membranes during relaxation. Recently, the antiapoptotic HS-1–associated protein X-1 (HAX-1) was identified as a binding partner of PLN, and this interaction was postulated to regulate cell apoptosis. In the current study, we determined that HAX-1 can also bind to SERCA2. Deletion mapping analysis demonstrated that amino acid residues 575–594 of SERCA2's nucleotide binding domain are required for its interaction with the C-terminal domain of HAX-1, containing amino acids 203-245. In transiently cotransfected human embryonic kidney 293 cells, recombinant SERCA2 was specifically targeted to the ER, whereas HAX-1 selectively concentrated at mitochondria. On triple transfections with PLN, however, HAX-1 massively translocated to the ER membranes, where it codistributed with PLN and SERCA2. Overexpression of SERCA2 abrogated the protective effects of HAX-1 on cell survival, after hypoxia/reoxygenation or thapsigargin treatment. Importantly, HAX-1 overexpression was associated with down-regulation of SERCA2 expression levels, resulting in significant reduction of apparent ER Ca2+ levels. These findings suggest that HAX-1 may promote cell survival through modulation of SERCA2 protein levels and thus ER Ca2+ stores. 相似文献
42.
Lalioti VS Vergarajauregui S Tsuchiya Y Hernandez-Tiedra S Sandoval IV 《Journal of cellular physiology》2009,218(2):416-426
We have previously reported the physical interaction between Daxx, the adaptor protein that mediates activation of the Jun amino-terminal kinase (JNK), and GLUT4, the insulin-dependent glucose transporter, interaction that involves their C-domains. Co-immunoprecipitation and two-hybrid-based protein-protein interaction studies show now that Daxx and GLUT4 interact with JNK1 through D-sites in their NH(2)-(aa 1-501) and large endofacial loop, respectively. Serum deprivation strongly enhances the association of JNK1 with Daxx and dissociates the kinase from GLUT4. SP600125, a potent JNK1 inhibitor, reduces the JNK1 activity associated with GLUT4 and the phosphorylation of two minor GLUT4 species in serum-starved 3T3-L1 adipocytes. In addition, Daxx interacts with kinesin KIF5B through the 6xTPR domain of the kinesin light chain, a domain engaged in the grab hold of protein cargo by kinesin motors that codistribute with JNK. Depletion of Daxx in 3T3-L1 adipocytes provokes the partial translocation of the GLUT4 retained in the GLUT4 storage compartment to endosomes. 相似文献
43.
Petros Katapodis Vasiliki Christakopoulou Paul Christakopoulos 《World journal of microbiology & biotechnology》2006,22(5):501-506
Summary A 32 central composite experimental design was performed with the aim of optimizing xylanase production by Thermomyces lanuginosus grown on corn cobs in submerged cultures. Xylanase production was first tested on different nitrogen sources (tomato skin,
tomato seed meal, corn steep liquor, meat peptone, bacto-tryptone and yeast extract). Tomato seed meal was the selected substrate
to test the effect of two variables on xylanase production (corn cobs and tomato seed meal concentrations). A second-order
quadratic model and a response surface method showed that the optimum condition for xylanase production was corn cobs 4.6%
(w/v) and tomato seed meal 2.1% (w/v). The optimum conditions found were transferred to 7-l bioreactors, where activities
as high as 1630 U/ml were obtained. 相似文献
44.
Anastasios Papalazarou Mary Yannakoulia Stavros A. Kavouras Vasiliki Komesidou George Dimitriadis Alexandros Papakonstantinou Labros S. Sidossis 《Obesity (Silver Spring, Md.)》2010,18(7):1348-1353
The present study was designed to evaluate the 3 year effects of a lifestyle intervention on weight loss and maintenance, dietary, and physical activity habits and eating behavior of patients following vertical banded gastroplasty (VBG). Thirty severely obese female volunteers were included in the study and they were randomly assigned to one of two intervention groups: usual care (UC) or lifestyle intervention (LS) group. Patients were followed for 3 years postoperatively. Outcome measures included weight loss, dietary habits, physical activity level (PAL), and eating behavior changes. Weight was significantly lower in the LS group after 12 months (84.4 ± 3.9 kg vs. 98.4 ± 4.4 kg, P < 0.05), 24 months (83.0 ± 3.3 vs. 101.9 ± 5.3 kg, P < 0.05), and 36 months following surgery (84.2 ± 3.3 vs. 102.5 ± 3.5 kg, P < 0.05). Repeated measures ANOVA revealed significant differences between the two groups overall and at specific time points for the PAL and TV viewing. With regard to eating behavior, the LS group scored significantly better in total Dutch Eating Behavior Questionnaire (DEBQ), Restraint Eating and External Eating scales at all postoperative time points. Similarly, significant differences were found between the two groups in dietary intake. These findings outline the importance of lifestyle intervention on weight loss and maintenance following bariatric surgery. The favorable effects of lifestyle intervention may be through adoption of healthier eating behaviors and increased physical activity. 相似文献
45.
Christodoulou V Messaritakis I Svirinaki E Tsatsanis C Antoniou M 《Experimental parasitology》2011,(3):279-284
Although macrophages have a microbicidal role in the immune system they themselves can be infected by pathogens. Often a simultaneous infection by more than one microbe may occur in a single cell. This is the first report of coinfection of macrophages with Toxoplasma gondii and Leishmania infantum, in vitro and in vivo. L. infantum does not cause severe disease in mice but T. gondii, RH strain, is lethal. Cell culture studies using THP-1 macrophages dually infected in vitro revealed that 4.3% harbored both parasites 24 h after infection. When mice were infected with both parasites on the same day 7.3% of the infected cells carried both parasites 7 days later. Yet, if mice were first infected with L. infantum and then with Toxoplasma (5 days post-infection) 18.7% of the macrophages hosted either parasite but concomitant infection could not be found and mice, already harboring L. infantum, survived Toxoplasma’s lethal effect. 相似文献
46.
Panagiotou V Love KR Jiang B Nett J Stadheim T Love JC 《Applied and environmental microbiology》2011,77(9):3154-3156
The selection of highly productive cell lines remains a key step for manufacturing therapeutic proteins. Microengraving was used to screen chemically mutagenized populations of Pichia pastoris for increased production of an Fc fragment. Clones retrieved following three rounds of mutagenesis yielded titers 2.65-fold greater than those of the parental strain. 相似文献
47.
Amillis S Kosti V Pantazopoulou A Mikros E Diallinas G 《Journal of molecular biology》2011,411(3):567-39605
Earlier, we identified mutations in the first transmembrane segment (TMS1) of UapA, a uric acid-xanthine transporter in Aspergillus nidulans, that affect its turnover and subcellular localization. Here, we use one of these mutations (H86D) and a novel mutation (I74D) as well as genetic suppressors of them, to show that TMS1 is a key domain for proper folding, trafficking and turnover. Kinetic analysis of mutants further revealed that partial misfolding and deficient trafficking of UapA does not affect its affinity for xanthine transport, but reduces that of uric acid and confers a degree of promiscuity towards the binding of other purines. This result strengthens the idea that subtle interactions among domains not directly involved in substrate binding refine the selectivity of UapA. Characterization of second-site suppressors of H86D revealed a genetic interaction of TMS1 with TMS3, the latter segment shown for the first time to be important for UapA function. Systematic mutational analysis of polar and conserved residues in TMS3 showed that Ser154 is crucial for UapA transport activity. Our results are in agreement with a topological model of UapA built on the recently published structure of UraA, a bacterial homolog of UapA. 相似文献
48.
A computational study of the Warburg effect identifies metabolic targets inhibiting
cancer migration
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Sylvia Zakkak Maria Chatzaki Nikos Karamalis Vasiliki Kati 《Journal of Insect Conservation》2014,18(4):599-611
Agricultural land abandonment is a major conservation issue when it comes to remote Mediterranean mountainous ecosystems. Although its impact on taxa such as birds or butterflies is well known, knowledge remains poor for less studied invertebrate taxa such as spiders. We sampled ground spiders (Gnaphosidae, Liocranidae, Miturgidae and Corrinidae) in 20 randomly selected sites (1 km × 1 km; 15 pitfall traps) that well depicted a land abandonment gradient, in terms of four forest encroachment classes. Our results showed a negative effect of land abandonment on ground spider species richness and diversity (Simpson index), pinpointing that forested habitats with more than 75 % woody vegetation cover are relatively poor. We also provide evidence for the beneficial role of low intensity grazing (0.4–4 livestock units/km2) for ground spider abundance. Community analysis revealed four distinct clusters of co-occurring species, while Generalized Linear Models at cluster and species level showed the definitive role of forest encroachment, and secondarily of other environmental factors such as humidity, elevation and longitude, in regulating species distribution patterns. Conservation measures for ground spider diversity maintenance should focus on promoting traditional agricultural practices, including small-scale cultivation and mild livestock grazing in order to preserve open and semi-open rural mosaics. 相似文献