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151.
Varsha K. Khodiyar Lois J. Maltais Barbara J. Ruef Katherine M.B. Sneddon Jennifer R. Smith Mary Shimoyama Fernando Cabral Charles Dumontet Susan K. Dutcher Robert J. Harvey Laurence Lafanechère John M. Murray Eva Nogales David Piquemal Fabio Stanchi Sue Povey Ruth C. Lovering 《Genomics》2009,93(4):397
152.
Water availability affects seasonal CO2‐induced photosynthetic enhancement in herbaceous species in a periodically dry woodland 下载免费PDF全文
Varsha S. Pathare Kristine Y. Crous Julia Cooke Danielle Creek Oula Ghannoum David S. Ellsworth 《Global Change Biology》2017,23(12):5164-5178
Elevated atmospheric CO2 (eCO2) is expected to reduce the impacts of drought and increase photosynthetic rates via two key mechanisms: first, through decreased stomatal conductance (gs) and increased soil water content (VSWC) and second, through increased leaf internal CO2 (Ci) and decreased stomatal limitations (Slim). It is unclear if such findings from temperate grassland studies similarly pertain to warmer ecosystems with periodic water deficits. We tested these mechanisms in three important C3 herbaceous species in a periodically dry Eucalyptus woodland and investigated how eCO2‐induced photosynthetic enhancement varied with seasonal water availability, over a 3 year period. Leaf photosynthesis increased by 10%–50% with a 150 μmol mol?1 increase in atmospheric CO2 across seasons. This eCO2‐induced increase in photosynthesis was a function of seasonal water availability, given by recent precipitation and mean daily VSWC. The highest photosynthetic enhancement by eCO2 (>30%) was observed during the most water‐limited period, for example, with VSWC <0.07 in this sandy surface soil. Under eCO2 there was neither a significant decrease in gs in the three herbaceous species, nor increases in VSWC, indicating no “water‐savings effect” of eCO2. Periods of low VSWC showed lower gs (less than ≈ 0.12 mol m?2 s?1), higher relative Slim (>30%) and decreased Ci under the ambient CO2 concentration (aCO2), with leaf photosynthesis strongly carboxylation‐limited. The alleviation of Slim by eCO2 was facilitated by increasing Ci, thus yielding a larger photosynthetic enhancement during dry periods. We demonstrated that water availability, but not eCO2, controls gs and hence the magnitude of photosynthetic enhancement in the understory herbaceous plants. Thus, eCO2 has the potential to alter vegetation functioning in a periodically dry woodland understory through changes in stomatal limitation to photosynthesis, not by the “water‐savings effect” usually invoked in grasslands. 相似文献
153.
Agarwal V Kommaddi RP Valli K Ryder D Hyde TM Kleinman JE Strobel HW Ravindranath V 《PloS one》2008,3(6):e2337
Cytochrome P450 (P450) is a super-family of drug metabolizing enzymes. P450 enzymes have dual function; they can metabolize drugs to pharmacologically inactive metabolites facilitating their excretion or biotransform them to pharmacologically active metabolites which may have longer half-life than the parent drug. The variable pharmacological response to psychoactive drugs typically seen in population groups is often not accountable by considering dissimilarities in hepatic metabolism. Metabolism in brain specific nuclei may play a role in pharmacological modulation of drugs acting on the CNS and help explain some of the diverse response to these drugs seen in patient population. P450 enzymes are also present in brain where drug metabolism can take place and modify therapeutic action of drugs at the site of action. We have earlier demonstrated an intrinsic difference in the biotransformation of alprazolam (ALP) in brain and liver, relatively more alpha-hydroxy alprazolam (alpha-OHALP) is formed in brain as compared to liver. In the present study we show that recombinant CYP3A43 metabolizes ALP to both alpha-OHALP and 4-hydroxy alprazolam (4-OHALP) while CYP3A4 metabolizes ALP predominantly to its inactive metabolite, 4-OHALP. The expression of CYP3A43 mRNA in human brain samples correlates with formation of relatively higher levels of alpha-OH ALP indicating that individuals who express higher levels of CYP3A43 in the brain would generate larger amounts of alpha-OHALP. Further, the expression of CYP3A43 was relatively higher in brain as compared to liver across different ethnic populations. Since CYP3A enzymes play a prominent role in the metabolism of drugs, the higher expression of CYP3A43 would generate metabolite profile of drugs differentially in human brain and thus impact the pharmacodynamics of psychoactive drugs at the site of action. 相似文献
154.
Alan Graham Joan Nowicke John J. Skvarla Shirley A. Graham Varsha Patel Sangtae Lee 《American journal of botany》1985,72(7):1012-1031
A palynological survey, including LM, SEM, and TEM, is presented for 10 genera of the Lythraceae s.s. (plus Alzatea to be assigned separate family status): Adenaria, Ammannia, Capuronia, Crenea, Cuphea, Decodon, Didiplis, Diplusodon, Galpinia, and Ginoria. Discussion of the results is deferred until the survey is complete, but already information of taxonomic interest is available for the 11 genera studied. The pollen data, consistent with results from cytology, anatomy, and embryology, suggest the current subgeneric classification into the Tribes Lythreae and Nesaeeae based on complete or incomplete septation of the ovary is not valid. The pollen of Alzatea is a generalized type and lacks the morphological features most characteristic of the Lythraceae (pseudocolpi, sculptured exine). The genus is being described as a separate family, and the pollen morphology is consistent with this treatment. The pollen of Cuphea is more diverse and shows little similarity to other genera in the family. It also has a unique combination of floral characters and lipid chemistry that suggests a separate category within the developing subfamilial classification of the Lythraceae. 相似文献
155.
Gene map of the extended human MHC 总被引:12,自引:0,他引:12
Horton R Wilming L Rand V Lovering RC Bruford EA Khodiyar VK Lush MJ Povey S Talbot CC Wright MW Wain HM Trowsdale J Ziegler A Beck S 《Nature reviews. Genetics》2004,5(12):889-899
The major histocompatibility complex (MHC) is the most important region in the vertebrate genome with respect to infection and autoimmunity, and is crucial in adaptive and innate immunity. Decades of biomedical research have revealed many MHC genes that are duplicated, polymorphic and associated with more diseases than any other region of the human genome. The recent completion of several large-scale studies offers the opportunity to assimilate the latest data into an integrated gene map of the extended human MHC. Here, we present this map and review its content in relation to paralogy, polymorphism, immune function and disease. 相似文献
156.
157.
Varsha Patel John J. Skvarla I. K. Ferguson Alan Graham Peter H. Raven 《American journal of botany》1985,72(3):407-413
Threadlike structures were observed on many pollen grains of Jacqueshuberia amplifoliola, J. purpurea, and J. quinquangulata of the Leguminosae/Caesalpinioideae. Each end was attached to the highly reticulate polar exine surface of different grains. The consistency of this observation, hence, the absence of free thread ends, is interpreted to mean that they should be considered as either exine bridges or exinial connections rather than viscin threads, although, most likely functionally homologous to viscin threads. Other significant morphological characters were the tricolporate apertures and strikingly lamellate foot layer and endexine. 相似文献
158.
BARD1 translocation to mitochondria correlates with Bax oligomerization, loss of mitochondrial membrane potential, and apoptosis 总被引:1,自引:0,他引:1
The breast cancer regulatory protein-1 (BRCA1)-associated RING domain 1 (BARD1) gene is mutated in a subset of breast/ovarian cancers. BARD1 functions as a heterodimer with BRCA1 in nuclear DNA repair. BARD1 also has a BRCA1-independent apoptotic activity. Here we investigated the link between cytoplasmic localization and apoptotic function of BARD1. We used immunofluorescence microscopy and deconvolution analysis to resolve BARD1 cytoplasmic staining patterns and detected endogenous BARD1 at mitochondria. BARD1 was also detected in mitochondrial cell fractions by immunoblotting. The targeting of BARD1 to mitochondria was modestly stimulated by DNA damage and did not require BRCA1 as indicated by RNA interference and peptide-competition experiments. Transiently expressed yellow fluorescence protein-BARD1 localized to mitochondria, and the targeting sequences were mapped to both the N and C terminus of BARD1. Ectopic yellow fluorescence protein-BARD1 induced apoptosis and loss of mitochondrial membrane potential in MCF-7 breast tumor cells. BARD1 apoptotic function was associated with stimulation of Bax oligomerization at mitochondria. This distinguishes it from BRCA1, which is pro-apoptotic but did not induce Bax oligomerization. The cancer-associated BARD1 splice-variant DeltaRIN (lacks the BRCA1 binding domain and ankyrin repeats) was recruited to mitochondria but did not stimulate apoptosis or alter membrane permeability. We propose that BARD1 has two main sites of action in its cellular response to DNA damage, the nucleus, where it promotes cell survival through DNA repair, and the mitochondria, where BARD1 regulates apoptosis. 相似文献
159.
Singh V Joshi D Shrivastava S Shukla S 《Indian journal of experimental biology》2007,45(12):1037-1044
Efficacy of thiol chelators viz. N-acetyl cysteine and D-penicillamine (NAC and DPA) along with nutritional supplements viz. zinc acetate, sodium selenite and magnesium sulphate (Zn, Se and Mg) in the treatment of mercury intoxication was investigated in rats. This is of particular interest since high bonding affinity between mercuric ion and the thiol group exits. The mutual antagonism of mercury and selenium is one of the strongest examples of the interaction in the trace element field. Adult rats of Sprague-Dawley strain were administered a bolus dose of dimethyl mercury (10 mg/kg) orally. A significant rise in the aspartate aminotransferase, alanine aminotransferase, serum alkaline phosphatase, lactate dehydrogenase, gamma glutamyltranspeptidase, bilirubin and creatinine were observed. Single mercury exposure also resulted in a significant increase in lipid peroxides with a concomitant decrease in reduced glutathione level in liver, kidney and brain. A decrease in the enzymatic activities of acetyl cholinesterase in different regions of the brain was observed. These parameters were restored considerably with chelating agents along with nutritional supplementation, but NAC+Se and DPA+Mg offered significant protection in comparison with other combinations. 相似文献
160.
Ramasamy SK Mailleux AA Gupte VV Mata F Sala FG Veltmaat JM Del Moral PM De Langhe S Parsa S Kelly LK Kelly R Shia W Keshet E Minoo P Warburton D Bellusci S 《Developmental biology》2007,307(2):237-247
The key role played by Fgf10 during early lung development is clearly illustrated in Fgf10 knockout mice, which exhibit lung agenesis. However, Fgf10 is continuously expressed throughout lung development suggesting extended as well as additional roles for FGF10 at later stages of lung organogenesis. We previously reported that the enhancer trap Mlcv1v-nLacZ-24 transgenic mouse strain functions as a reporter for Fgf10 expression and displays decreased endogenous Fgf10 expression. In this paper, we have generated an allelic series to determine the impact of Fgf10 dosage on lung development. We report that 80% of the newborn Fgf10 hypomorphic mice die within 24 h of birth due to respiratory failure. These mutant mouse lungs display severe hypoplasia, dilation of the distal airways and large hemorrhagic areas. Epithelial differentiation and proliferation studies indicate a specific decrease in TTF1 and SP-B expressing cells correlating with reduced epithelial cell proliferation and associated with a decrease in activation of the canonical Wnt signaling in the epithelium. Analysis of vascular development shows a reduction in PECAM expression at E14.5, which is associated with a simplification of the vascular tree at E18.5. We also show a decrease in α-SMA expression in the respiratory airway suggesting defective smooth muscle cell formation. At the molecular level, these defects are associated with decrease in Vegfa and Pdgfa expression likely resulting from the decrease of the epithelial/mesenchymal ratio in the Fgf10 hypomorphic lungs. Thus, our results indicate that FGF10 plays a pivotal role in maintaining epithelial progenitor cell proliferation as well as coordinating alveolar smooth muscle cell formation and vascular development. 相似文献