全文获取类型
收费全文 | 127篇 |
免费 | 7篇 |
出版年
2022年 | 5篇 |
2021年 | 2篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 5篇 |
2012年 | 17篇 |
2011年 | 10篇 |
2010年 | 5篇 |
2009年 | 5篇 |
2008年 | 8篇 |
2007年 | 7篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 11篇 |
2003年 | 7篇 |
2002年 | 8篇 |
2001年 | 1篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 4篇 |
1997年 | 1篇 |
1994年 | 1篇 |
1993年 | 3篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有134条查询结果,搜索用时 15 毫秒
51.
52.
Perinatal exposures to endocrine-disrupting chemicals, such as polychlorinated biphenyls (PCBs), can cause latent effects on reproductive function. Here, we tested whether PCBs administered during late pregnancy would compromise reproductive physiology in both the fetally exposed female offspring (F1 generation), as well as in their female offspring (F2 generation). Pregnant Sprague-Dawley rats were treated with the PCB mixture, Aroclor 1221 (A1221; 0, 0.1, 1, or 10 mg/kg), on Embryonic Days 16 and 18. Somatic and reproductive development of F1 and their F2 female offspring were monitored, including ages of eye opening, pubertal landmarks, and serum reproductive hormones. The results showed that low doses of A1221 given during this critical period of neuroendocrine development caused differential effects of A1221 on F1 and F2 female rats. In both generations, litter sex ratio was skewed toward females. In the F1 generation, additional effects were found, including a significant alteration of serum LH in the 1 mg/kg A1221 group. The F2 generation showed more profound alterations, particularly with respect to fluctuations in hormones and reproductive tract tissues across the estrous cycle. On proestrus, the day of the preovulatory GnRH/gonadotropin surge, F2 females whose mothers had been exposed perinatally to A1221 exhibited substantially suppressed LH and progesterone concentrations, and correspondingly smaller uterine and ovarian weights on estrus, compared with F2 descendants of control rats. These latter changes suggest a dysregulation of reproductive physiology. Thus, low levels of exposure to PCBs during late fetal development cause significant effects on the maturation and physiology of two generations of female offspring. These findings have implications for reproductive health and fertility of wildlife and humans. 相似文献
53.
54.
John P. Wares Deena S. Goldwater † Bo Y. Kong & Clifford W. Cunningham 《Ecology letters》2002,5(4):577-584
Human activities have strongly impacted natural communities through the introduction of non-native species in historical times. A frequently cited marine example is Littorina littorea , a common intertidal gastropod that was first reported in North America in 1840. The seemingly sudden appearance and rapid geographical spread of this species southward from Nova Scotia has led many researchers to consider L. littorea a human-mediated species introduction. This is despite allozyme and subfossil evidence that the `European periwinkle' was in North America long before 1840. Our mitochondrial and nuclear DNA sequence data confirm that L. littorea has been in continuous residence in North America for at least 8000 years. It appears most likely that ecological interactions, rather than oceanographic or climatic forces, maintained the limited geographical distribution of L. littorea prior to the 19th century. 相似文献
55.
Linda A. Castle Deena Errampalli Tammy L. Atherton Linda H. Franzmann Elizabeth S. Yoon David W. Meinke 《Molecular genetics and genomics : MGG》1993,241(5-6):504-514
Over 5000 transgenic families of Arabidopsis thaliana produced following seed transformation with Agrobacterium tumefaciens were screened for embryonic lethals, defectives, and pattern mutants. One hundred and seventy-eight mutants with a wide range of developmental abnormalities were identified. Forty-one mutants appear from genetic studies to be tagged (36% of the 115 mutants examined in detail). Mapping with visible markers demonstrated that mutant genes were randomly distributed throughout the genome. Seven mutant families appeared to contain chromosomal translocations because the mutant genes exhibited linkage to visible markers on two different chromosomes. Chromosomal rearrangements may therefore be widespread following seed transformation. DNA gel blot hybridizations with 34 tagged mutants and three T-DNA probes revealed a wide range of insertion patterns. Models of T-DNA structure at each mutant locus were constructed to facilitate gene isolation. The value of such models was demonstrated by using plasmid rescue to clone flanking plant DNA from four tagged mutants. Further analysis of genes isolated from these insertional mutants should help to elucidate the relationship between gene function and plant embryogenesis. 相似文献
56.
Genetic and molecular characterization of embryonic mutants identified following seed transformation in Arabidopsis 总被引:6,自引:0,他引:6
Linda A. Castle Deena Errampalli Tammy L. Atherton Linda H. Franzmann Elizabeth S. Yoon David W. Meinke 《Molecular & general genetics : MGG》1993,241(5-6):504-514
Over 5000 transgenic families of Arabidopsis thaliana produced following seed transformation with Agrobacterium tumefaciens were screened for embryonic lethals, defectives, and pattern mutants. One hundred and seventy-eight mutants with a wide range of developmental abnormalities were identified. Forty-one mutants appear from genetic studies to be tagged (36% of the 115 mutants examined in detail). Mapping with visible markers demonstrated that mutant genes were randomly distributed throughout the genome. Seven mutant families appeared to contain chromosomal translocations because the mutant genes exhibited linkage to visible markers on two different chromosomes. Chromosomal rearrangements may therefore be widespread following seed transformation. DNA gel blot hybridizations with 34 tagged mutants and three T-DNA probes revealed a wide range of insertion patterns. Models of T-DNA structure at each mutant locus were constructed to facilitate gene isolation. The value of such models was demonstrated by using plasmid rescue to clone flanking plant DNA from four tagged mutants. Further analysis of genes isolated from these insertional mutants should help to elucidate the relationship between gene function and plant embryogenesis. 相似文献
57.
58.
Deena S. Decker-Walters Mary Wilkins-Ellert Sang-Min Chung Jack E. Staub 《Economic botany》2004,58(4):501-508
Bottle gourd /Lagenaria siceraria (Mol.) Standley] is an edible, medicinal, and otherwise utilitarian domesticated cucurbit
with an ancient pantropical distribution. This African native reached Asia and the Americas 9000 years ago, probably as a
wild species whose fruits had floated across the seas. Independent domestications from wild populations are believed to have
occurred in both the Old and New Worlds. However, few wild populations of L. siceraria have been found during recorded history
and none has been verified or studied in detail. In 1992, Mary Wilkins-Ellert discovered an unusual free-living plant of Lagenaria
in a remote region of southeastern Zimbabwe. Her morphological observations during several plantings of the collected seed,
as well as results from two genetic analyses (random amplified polymorphic DNA and chloroplast sequencing), indicate that
the Zimbabwe collection is part of a genetically distinct and wild lineage ofL. siceraria. 相似文献
59.
60.
The yeast Saccharomyces cerevisiae is a common model organism for biological discovery. It has become popularized primarily because it is biochemically and genetically amenable for many fundamental studies on eukaryotic cells. These features, as well as the development of a number of procedures and reagents for isolating protein complexes, and for following macromolecules in vivo, have also fueled studies on nucleo-cytoplasmic transport in yeast. One limitation of using yeast to study transport has been the absence of a reconstituted in vitro system that yields quantitative data. However, advances in microscopy and data analysis have recently enabled quantitative nuclear import studies, which, when coupled with the significant advantages of yeast, promise to yield new fundamental insights into the mechanisms of nucleo-cytoplasmic transport. 相似文献