白簕叶挥发油的化学成分

用GCMS联用技术首次研究了云南西双版纳白簕叶挥发油的化学成分,并应用色谱峰面积归一化法计算各成分的相对含量。一共分离出108个峰,确认了其中的81种成分,所鉴定的组分占挥发油总量的96.50%,主要是萜烯类及其含氧衍生物等。     

垂体瘤转化基因1(PTTG1)的表达变化对人前列腺癌细胞LNCaP生长增殖的影响

垂体肿瘤转化基因1(PTTG1)具有促进肿瘤生长和转移的作用.通过上调或下调基因表达的策略,观察PTTG1基因对人前列腺癌细胞株LNCaP细胞生长增殖的影响.利用PCR技术分离出PTTG1全长cDNA,分别正向和反向插入真核表达载体pIRES2-EGFP,重组载体分别命名为正义PTTG1-S/pIRES2-EGFP(即pI-P-S)和反义PTTG1-AS/pIRES2-EGFP(即pI-P-AS),将这两种重组载体稳定转染LNCaP细胞,通过流式细胞仪和MTT法分别检测了细胞周期和细胞增殖的情况.转染正义PTTG1后处于S期和G2期的细胞明显增加,细胞生长增殖能力增强;相反,转染反义PTTG1后处于S期和G2期细胞明显减少,细胞生长增殖能力减弱(P<0.05).结果表明,PTTG1能明显改变人前列腺癌细胞株LNCaP的细胞周期和细胞生长增殖能力,它的异常表达可能参与前列腺癌细胞生长增殖过程.     

GFAP and PCNA Marking in the cerebellum of masu salmon’s (<Emphasis Type="Italic">Oncorhynchus masou</Emphasis>) juvenile after mechanical injury

The objective of this work was to study proliferation processes and the role of glia and neural stem cells in the event of injurious action on cerebellum of masu salmon’s (Oncorhynchus masou) juvenile. Using the immunoperoxidase staining of the glial fibrillary acidic protein (GFAP) and proliferating cells nuclear antigen (PCNA), processes of proliferation and gliogenesis after mechanical trauma of cerebellum of cherry salmon’s (Oncorhynchus masou) juvenile were studied. After the trauma, the intensity of proliferation and migration processes varies in different zones. Proliferation processes decrease after the trauma in lateral and basal zones, and migration increases. In the dorsal zone, on the contrary, migration processes significantly decrease and proliferation increases. In the dorsal matrix zone of a cerebellum, intense cell proliferation was detected. In the dorsal, lateral, and basal zone of the molecular layer of cerebellum after traumatic damage, neurogenic niches containing PCNA and cells, as well as a heterogeneous population of PCNA-cells, were identified. At the location of neurogenic niches, fibers of radial glia and small single intensely or moderately labeled GFAP cells were discovered. As a result of damaging action, GFAP+ fibers of radial glia, which form differently directed radially oriented bundles, appeared in the dorsal matrix zone. Such structural formations have not been discovered in intact animals. We suppose that, after the trauma, structural reconstruction connected with partial spatial reorientation of the radial glia fibers and formation of specific directions for cells formed in this zone occurs in the dorsal matrix zone. As a result of the trauma, in masu salmon’s cerebellum, elements of the radial glia, including both cells possessing typical morphology and cell fragments presented as long radially oriented processes or cell body containing initial fragments of radial fibers, appeared.     

4种桦树幼苗耐盐性分析与评价

耐盐植物引种和培育是开发利用盐碱地的主要方式,具有重要的研究价值。本试验以哈萨克斯坦引进的吉尔吉斯白桦（Betula kirghisorum）、欧洲白桦（B.pendula）、毛枝桦（B.pubescens）和本地的白桦（B.platyphylla Suk.）1年生幼苗为试验材料,于2014年7月在东北林业大学进行中性盐（NaCl）和碱性盐（NaHCO₃）的胁迫试验,测定生长量、光合参数和叶绿素含量,并通过因子分析法,对比评价4种桦树幼苗的耐盐碱能力,筛选出综合性状优良的桦树树种,为耐盐植物引种和培育提供有价值的数据。结果表明：随着盐浓度的升高,桦树幼苗的高生长和光合效率受到显著抑制,而当浓度≥0.5%时,大部分幼苗枯死。株高增长量、基径增长量、净光合速率（P_n）、光能利用效率（SUE）、羧化效率（CUE）、表观量子效率（AQY）及叶绿素含量之间的相关性多数达到了显著水平;最后利用因子分析法分别构建了0.1% NaCl、0.3% NaCl、0.1% NaHCO3和0.3% NaHCO₃胁迫处理的综合评价公式,并分别筛选出了综合性状相对优良的单株,其中NaCl胁迫下较优单株为32、33、34、35;NaHCO₃胁迫下较优单株为262、263、264、35。综合比较认为,吉尔吉斯白桦对低中浓度的中性盐的抗性最强,本地对照白桦对低中浓度碱性盐的抗性最强,而吉尔吉斯白桦和毛枝桦对高浓度碱性盐抗性较强。     

Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array

Renal cell carcinoma (RCC) is a common uro- genital malignancy and often shows odd biological features. RCC accounts for approximately 2% of ma- lignancies worldwide. The incidence of and mortality from RCC have continuously increased during the last 50 years. One third of the patients already have me- tastases when first consulting the doctors. Another 30%—40% of patients develop metastasis after surgi- Identification of over-expressed genes in human RCC 149 cal excision of the pri…     

Nitroxidergic Cells in the Intestine of the Bivalve Mollusk Modiolus kurilensis

The distribution of nitroxidergic (NO-ergic) cells in the direct and recurrent loops of the midgut and in the hindgut of the bivalve mollusk Modiolus kurilensis was studied using a NADPH-diaphorase (NADPH-d) histochemistry [13]. Intraepithelial NO-ergic cells were found in the intestinal groove, in the major typhlosole of the direct loop, in the recurrent loop of the midgut, as well as in the hindgut. The apical processes of these cells are directed to the intestinal lumen, whereas the basal processes connect to the basiepithelial NO-ergic plexus. The latter, in turn, have separate fibers that are in contactswith the subepithelial NO-ergic plexus. Both plexuses are well developed in the intestinal groove of the direct loop and in the recurrent loop of the midgut, as well as in the hindgut. In the major typhlosole and crystalline style sac of the direct loop, both basi- and subepithelial NO-ergic plexuses are less developed. In the minor typhlosole, the basiepithelial NO-ergic plexus is absent and only single fibers of the subepithelial plexus occur. In the connective tissue and muscular layers of the recurrent loop of the midgut, subepithelial NO-ergic nerve cells were found.     

腺花香茶菜中的对映-贝壳杉烯类二萜化合物

从昆明产腺花香茶菜(Isodon adenanthus (Diels) Kudo)的地上部分分离到8个化合物,通过波谱分析鉴定,化合物1-3为新的对映-贝壳杉烯类二萜化合物,命名为腺花香茶菜素N、0和P;4个已知二萜为白叶香茶菜戊素(4)、无毛狭叶香茶菜素C(5)、腺花香茶菜甲素(6)和白叶香茶菜乙素(7),同时得到一个高度不饱和脂肪酸9,16-二羰基-10,12,14-三烯-十八碳酸(8)。根据ROESY波谱,对化合物4的结构进行了修正。化合物1对K562细胞显示出明显的细胞毒活性(IC50=0．45μg／mL)。     

NO-Ergic Innervation of the Intestine of the Snow Sculpin Myoxocephalus brandti

Distribution, localization, and morphological peculiarities of NO-ergic nerve cells in the intestine of the snow sculpin Myoxocephalus brandti (Cottidae family) were studied using histochemical staining for NADPH-diaphorase ( NADPH-d). These cells were shown to be present in the pyloric appendages, middle and posterior parts of the intestine and in its rectal part. The NO-ergic cells are the most numerous in the myenteric plexus and circular muscle layer of all studied parts of the intestine. Single NO-ergic nerve cells are revealed in the submucosal plexus of pyloric appendages, middle and posterior parts of the intestine. No NO-ergic neural cells were found in subserosal and subepithelial plexuses, longitudinal layer of smooth muscle in all studied parts, and in the submucosal plexus of the rectal part of the intestine.     

Neuronal Organization of the Telencephalon in the Chum Salmon Onchorhynchus keta

This paper presents original data on the neuronal composition of various regions of the pallium of the telencephalon in Oncorhynchus keta. This study was carried out using routine neurohistologic techniques. Four basic areas were distinguished within the pallium: medial, dorsal, central, and lateral. The central pallium exhibited the most complicated cytoarchitectonics. In the central and lateral areas, pyramidal-like neurons with a well-developed dendritic spine apparatus were found. These were allodendritic cells, which appeared to be comparable to the pyramidal neurons of higher vertebrates in a number of features. Horizontal neurons and isodendritic radial neurons were also encountered. These cell types occurred in the ventral part of the central area and in the lateral area.     

GABA transporter 1 transcriptional starting site exhibiting tissue specific difference

INTRODUCTIONIn the vertebrate central nervous system (CNS),GABA transporters (GAT) are believed to play animportant role in termination of GABAergiC transInission. GATI was the first cloned member of neurotransmitter transporters superfanilly[1], and soon,other three subtypes (GAT2-4) were subsequentlycloned. Since GABA is the predominant inhibitoryneurotranslliltter in CNS, abnormallty of GATs hasa direct relationship with certain kinds of nervousdisorders, such as epilepsy a…