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排序方式: 共有194条查询结果,搜索用时 15 毫秒
101.
Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies 总被引:29,自引:0,他引:29
Stöger E Vaquero C Torres E Sack M Nicholson L Drossard J Williams S Keen D Perrin Y Christou P Fischer R 《Plant molecular biology》2000,42(4):583-590
This report describes the stable expression of a medically important antibody in the staple cereal crops rice and wheat. We successfully expressed a single-chain Fv antibody (ScFvT84.66) against carcinoembryonic antigen (CEA), a well characterized tumor-associated marker antigen. scFv constructs were engineered for recombinant antibody targeting to the plant cell apoplast and ER. Up to 30 g/g of functional recombinant antibody was detected in the leaves and seeds of wheat and rice. We confirmed that transgenic dry seeds could be stored for at least five months at room temperature, without significant loss of the amount or activity of scFvT84.66. Our results represent the first transition from model plant expression systems, such as tobacco and Arabidopsis, to widely cultivated cereal crops, such as rice and wheat, for expression of an antibody molecule that has already shown efficacy in clinical applications. Thus, we have established that molecular pharming in cereals can be a viable production system for such high-value pharmaceutical macromolecules. Our findings provide a strong foundation for exploiting alternative uses of cereal crops both in industrialized and developing countries. 相似文献
102.
Tocotrienols are a group of natural vitamin E compounds with patent antitumoral effects, mostly based on their ability to induce apoptosis in cancer cells. In activated pancreatic stellate cells (PSCs) we have determined that tocotrienols elicit a dramatic mitochondrial destabilization followed by initiation of non-necrotic forms of programmed cell death, namely apoptosis and autophagy. PSCs are the main cell type involved in the generation of pancreatic fibrosis, and their removal is critical to limit the fibrogenic process. Noteworthy, tocotrienol death-promoting actions are exclusively directed to activated PSCs, but not to their quiescent counterparts nor to terminally differentiated acinar cells. Here, we hypothesize that the transformed phenotype of PSCs may include "activated" mitochondria, which can be used by tocotrienols to trigger autophagic and apoptotic signaling. We propose that mitochondria are the cornerstone of cell sensitivity to tocotrienols, and suggest possible mechanisms, that may be interconnected, on how tocotrienols may govern mitochondrial death pathways. 相似文献
103.
104.
Sirtuins are crucial proteins involved in sensing and coordinating the response to different forms of stress, mainly through NAD+‐dependent deacetylation of proteins. For that reason, sirtuins are directly involved in many human pathologies including cancer, diabetes, cardiovascular and neurodegenerative diseases. SirT7, one of the less well‐understood sirtuins, has been associated with ribosome biogenesis, gene expression, metabolism and cancer. Despite the wide range of these functions, only a handful of targets for SirT7 have so far been described. In this issue, Zhang et al. report the first proteomic screening of SirT7 substrates. Using stable isotope labeling with amino acids in cell culture (SILAC), coupled with quantitative mass spectrometry, they have identified a comprehensive list of candidates involved in a variety of functions, ranging from maintenance of chromatin architecture to gene silencing and metabolism. A selected group of these candidates has been validated by in vitro co‐immunoprecipitation and deacetylation experiments. Predictive tools have identified additional candidates. The identification of these novel targets not only suggests new ways of understanding the physiological role of SirT7, but also provides new evidence to add to our existing knowledge of the global impact of sirtuins in cell homeostasis. 相似文献
105.
Rampant horizontal transfer and duplication of rubisco genes in eubacteria and plastids 总被引:14,自引:2,他引:14
Previous work has shown that molecular phylogenies of plastids,
cyanobacteria, and proteobacteria based on the rubisco (ribulose-1,5-
bisphosphate carboxylase/oxygenase) genes rbcL and rbcS are incongruent
with molecular phylogenies based on other genes and are also incompatible
with structural and biochemical information. Although it has been much
speculated that this is the consequence of a single horizontal gene
transfer (of a proteobacterial or mitochondrial rubisco operon into
plastids of rhodophytic and chromophytic algae), neither this hypothesis
nor the alternative hypothesis of ancient gene duplication have been
examined in detail. We have conducted phylogenetic analyses of all
available bacterial rbcL sequences, and representative plastid sequences,
in order to explore these alternative hypothesis and fully examine the
complexity of rubisco gene evolution. The rbcL phylogeny reveals a
surprising number of gene relationships that are fundamentally incongruent
with organismal relationships as inferred from multiple lines of other
molecular evidence. On the order of six horizontal gene transfers are
implied by the form I (L8S8) rbcL phylogeny, two between cyanobacteria and
proteobacteria, one between proteobacteria and plastids, and three within
proteobacteria. Alternatively, a single ancient duplication of the form I
rubisco operon, followed by repeated and pervasive differential loss of one
operon or the other, would account for much of this incongruity. In all
probability, the rubisco operon has undergone multiple events of both
horizontal gene transfer and gene duplication in different lineages.
相似文献
106.
José María Mateos-Pérez Carmen García-Villalba Javier Pascau Manuel Desco Juan J. Vaquero 《PloS one》2013,8(8)
We present jClustering, an open framework for the design of clustering algorithms in dynamic medical imaging. We developed this tool because of the difficulty involved in manually segmenting dynamic PET images and the lack of availability of source code for published segmentation algorithms. Providing an easily extensible open tool encourages publication of source code to facilitate the process of comparing algorithms and provide interested third parties with the opportunity to review code. The internal structure of the framework allows an external developer to implement new algorithms easily and quickly, focusing only on the particulars of the method being implemented and not on image data handling and preprocessing. This tool has been coded in Java and is presented as an ImageJ plugin in order to take advantage of all the functionalities offered by this imaging analysis platform. Both binary packages and source code have been published, the latter under a free software license (GNU General Public License) to allow modification if necessary. 相似文献
107.
Raul A. Wapnir Gerard Devas Cecilia Vaquero Solans 《Biological trace element research》1993,36(3):291-305
l-histidine (His) has been shown to enhance the inhibitory effect of zinc on intestinal copper absorption. This study was aimed
at examining whether this effect of His was also extended to the interactions of other divalent cations: ferrous iron, tin,
and cobalt, using an in vivo perfusion system in rats. Copper absorption and intestinal content of this element significantly
decreased in the presence of 2 mM His and ferrous iron. Iron accumulation was greater when His was present than when omitted. A fivefold excess of tin inhibited
copper absorption only when His was present. Citrate, at the same concentration as His, had no effect on copper absorption,
but hepatic copper levels were increased, as compared to the absence of either His or citrate. Addition of 0.5 or 1.0 mM cobaltous salt plus His resulted in a sharp decrease in copper intestinal absorption, with an increase in intestinal tissue
retention. These results confirm earlier findings with zinc and His, and suggest that a general phenomenon, either accelerating
the removal of copper from the intestinal lumen or increasing, the retention of this element by the intestinal tissue, is
a common feature of the interaction between cations of similar electronic configuration to copper and a high-affinity ligand,
such as His. 相似文献
108.
Vaquero Perea Cristina Valverde-Asenjo Inmaculada Vázquez de la Cueva Antonio Martín-Sanz Juan Pedro Molina José Antonio Quintana José Ramón 《Plant Ecology》2020,221(11):1143-1158
Plant Ecology - Understanding soil responses to plant colonization is important for managing abandoned lands. We investigated the influence of species colonizing abandoned fields on soil components... 相似文献
109.
Richard A Stabler Lisa F Dawson Petra CF Oyston Richard W Titball Jim Wade Jason Hinds Adam A Witney Brendan W Wren 《BMC microbiology》2008,8(1):177
Background
Human and animal health is constantly under threat by emerging pathogens that have recently acquired genetic determinants that enhance their survival, transmissibility and virulence. We describe the construction and development of an Active Surveillance of Pathogens (ASP) oligonucleotide microarray, designed to 'actively survey' the genome of a given bacterial pathogen for virulence-associated genes. 相似文献110.
Vincenzo Trotta Federico CF Calboli Marcello Ziosi Daniela Guerra Maria C Pezzoli Jean R David Sandro Cavicchi 《BMC evolutionary biology》2006,6(1):67