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Murraya koenigii (L.) Spreng., commonly known as curry leaf plant, is found in the different hilly regions of India. In the present study, fifty-nine accessions representing eight wild populations of M. koenigii were analyzed using thirteen ISSR primers. A total of 152 bands were amplified, out of which, 136 were polymorphic corresponding to 89.47% polymorphism across the accessions. The pairwise population genetic distances were calculated for all the populations that varied from 0.05 to 0.13 between the populations of M. koenigii. AMOVA and Nei’s genetic diversity analysis revealed higher genetic variations within populations than among the populations. The clustering of populations in the dendrogram was not in congruence with geographical affiliations. The results indicate that the ISSR method is sufficiently informative and powerful to estimate the genetic diversity in M. koenigii populations. As M. koenigii is an important wild plant genetic resource, therefore, information on genetic variability might be a potential source as breeding material for development of commercially valuable traits in M. koenigii plants. 相似文献
965.
We examined the possibility of using fish chromatophores as markers of arsenic exposure. Our results not only favor their suitability but also suggest an adaptive mechanism in fish against arsenic toxicity. We also compare results between arsenic accumulation, melanophore index and neurosecretory cells. Chromatophores can be used as a quick and reliable biomarker of aquatic metal pollution. 相似文献
966.
D. Pan A. K. Bera S. Das S. Bandyopadhyay T. Rana S. Bandyopadhyay S. K. Das D. Bhattacharya 《Molecular biology reports》2010,37(5):2223-2226
Nitric Oxide (NO) plays an important role for regulation of cellular and vascular response of inflammation and initiates killing
mechanism in the host-defense reactions. NO production is regulated through inducible nitric oxide synthase (iNOS) pathway
in response to infections and injurious agents besides pro-inflammatory cytokines secreted by the host. Evaluation of NO pathway
is one of the major targets which can evaluate various immunomodulators for their therapeutic interaction on innate immune
system. Lipopolysaccharide (LPS) and concavalin A (ConA) are used as standard mitogen for peripheral blood mononuclear cells
and splenocyte of mammalian and avian cell culture. During the present investigation ZnCl2 has been explored as standard mitogen and result was comparable with standard mitogen. The result has been evaluated on the
basis of relative mRNA expression of iNOS and interferon gamma and nitrite assay. Observation indicated significantly higher
expression of both biomolecules in comparison to control, LPS, ConA treated group. This study indicated that, ZnCl2 can also be used as standard stimulant for molecular mining of innate immunity. 相似文献
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968.
Khushi L. Matta Surjit S. Rana Conrad F. Piskorz Joseph J. Barlow 《Carbohydrate research》1983,112(2):213-220
Reaction of 2,3,4-tri-O-acetyl-α-l-fucopyranosyl bromide with p-nitrophenyl 3,4-O-isopropylidene-β-d-fucopyranoside in the presence of mercuric cyanide in acetonitrile, followed by removal of the isopropylidene group under mild conditions, gave a mixture of p-nitrophenyl 2-O-(2,3,4-tri-O-acetyl-α- and -β-l-fucopyranosyl)-β-d-fucopyranoside. These compounds were conveniently separated by preparative, thin-layer chromatography, and, on deacetylation, gave the title disaccharides, whose structures were established by 1H- and 13C-n.m.r. spectroscopy. 相似文献
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Sánchez FA Rana R González FG Iwahashi T Durán RG Fulton DJ Beuve AV Kim DD Durán WN 《The Journal of biological chemistry》2011,286(35):30409-30414
Endothelial NOS (eNOS)-derived NO is a key factor in regulating microvascular permeability. We demonstrated previously that eNOS translocation from the plasma membrane to the cytosol is required for hyperpermeability. Herein, we tested the hypothesis that eNOS activation in the cytosol is necessary for agonist-induced hyperpermeability. To study the fundamental properties of endothelial cell monolayer permeability, we generated ECV-304 cells that stably express cDNA constructs targeting eNOS to the cytosol or plasma membrane. eNOS-transfected ECV-304 cells recapitulate the eNOS translocation and permeability properties of postcapillary venular endothelial cells (Sánchez, F. A., Rana, R., Kim, D. D., Iwahashi, T., Zheng, R., Lal, B. K., Gordon, D. M., Meininger, C. J., and Durán, W. N. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 6849-6853). We used platelet-activating factor (PAF) as a proinflammatory agonist. PAF activated eNOS by increasing phosphorylation of Ser-1177 and inducing dephosphorylation of Thr-495, increasing NO production, and elevating permeability to FITC-dextran 70 in monolayers of cells expressing wild-type and cytosolic eNOS. PAF failed to increase permeability to FITC-dextran 70 in monolayers of cells transfected with eNOS targeted to the plasma membrane. Interestingly, this occurred despite eNOS Ser-1177 phosphorylation and production of comparable amounts of NO. Our results demonstrate that the presence of eNOS in the cytosol is necessary for PAF-induced hyperpermeability. Our data provide new insights into the dynamics of eNOS and eNOS-derived NO in the process of inflammation. 相似文献