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991.
Fructose metabolism in four Pseudomonas species 总被引:1,自引:0,他引:1
1. ATP-Dependent phosphorylation of fructose could not be detected in extracts of fructose-grown cells of Pseudomonas extorquens strain 16, Pseudomonas 3A2, Pseudomonas acidovorans and Pseudomonas fluorescens. Instead, phosphorylation of fructose to fructose-1-phosphate was found to occur when cell-free extracts were incubated with fructose and phosphoenolpyruvate. Such an activity could not be detected in cell-free extracts of succinate-grown cells. 2. High levels of 1-phosphofructokinase were found in extracts of the above organisms when growth on fructose. 3. Mutants of Pseudomonas extorquens strain 16 lacking 1-phosphofructokinase were unable to grow on fructose. Revertants to growth on fructose had regained the capacity to synthesize this enzyme, indicating its necessary involvement in fructose metabolism. 4. A survey has been carried out of enzymes involved in carbohydrate metabolism in the species listed above. 相似文献
992.
Ferdinand Bohlmann Karl-Heinz Knoll Christa Zdero Pradip Kumar Mahanta Michael Grenz Albert Suwita Dorothea Ehlers Ngo Le Van Wolf-Rainer Abraham Arvind Anant Natu 《Phytochemistry》1977,16(7):965-985
Investigation of about 50 Senecio species has afforded many new substances, in addition to known compounds. Present in these plants are 23 fura 相似文献
993.
Susan Van Noorden Julia M. Polak A. G. E. Pearse 《Histochemistry and cell biology》1977,53(3):243-247
Summary Immunostaining of thin serial paraffin sections has shown that somatostatin is present in the same parafollicular cells as calcitonin in the adult rat thyroid gland. The number of cells containing both peptides is much smaller than the number containing calcitonin but not somatostatin. 相似文献
994.
Escherichia coli nucleoids were visualized in a compact, highly supercoiled conformation at 1 M NaCl. After the salt concentration was lowered to 0.15 M NaCl, the nucleoids uncoiled in beaded fibers in which RNA-mediated structural domains could be distinguished. 相似文献
995.
996.
Thoralf M. Sundt Jr. Robert E. Anderson Russell A. Van Dyke 《Journal of neurochemistry》1978,31(3):627-635
Brain tissue pH was measured in cats at normocapnia hypocapnia, hypercapnia, and death from anoxia using a pH sensitive fluorescent indicator (umbelliferone) with both molecular and ionic fluorophors. A ratio analysis of the indicator's calibrated 450 nm fluorescent tissue washout curves from 340 and 370 nm excitation permitted direct determinations using a nomogram. Possible errors in these measurements related to differential quenching, absorption, and changes in the redox state of the indicator were investigated in vitro and in vivo for brain tissue and blood. In animals with preserved autoregulation, brain pH varied linearly with arterial pH (art pH 7.0, brain pH 6.98: art pH 7.4, brain pH 7.24). Brain pH at death fell to 6.68. An analysis of the indicator clearance curves suggests these measurements reflect a component of the intracellular space and the lipid solubility of the indicator suggests this is a membranous component. 相似文献
997.
P Van Ham 《Journal of theoretical biology》1978,72(2):269-281
In this paper some earlier stated theorems on logical control networks are proved. It is proved that a cascade of elements is equivalent to a single positive or negative element with N, number of negative elements, respectively even or odd. A loop of elements may show a cyclic behaviour with N odd or may show two possible stationary states with N even. A grafted cascade on a loop destroys the typical behaviour of the loop if the cascade is a negative chain grafted with an AND or a positive chain grafted with an OR connection; the system then admits only one stable state. Grafting a negative chain with an OR or a positive chain with an AND connection leaves the general behaviour of the loop unaffected. The detailed behaviour of two interconnected loops is extensively described. Finally it is indicated that although a complex network can be formalized as a reduced graph, its topological properties cannot always predict the final possible behaviour. 相似文献
998.
Implications of a model where the formation of two mediators interacting through cross inhibition is stimulated by the same hormone or by two specific hormones are analyzed at steady'state, first in the case where no co-operative processes are involved, secondly in the case where one of the inhibitory branches presents positive co-operativity characteristics. The possible occurrence of agonist concentration-mediators response curves with extrema of opposite type, multiple steady states, hysteresis and discontinuous transitions from one functional program to another is demonstrated. When steady-state hormonal levels fluctuate, it is shown that the cross inhibitory mediators may induce a strict temporal organization of the intracellular processes and result in both amplification and frequency multiplication of the fluctuations. 相似文献
999.
Human β-endorphin administered intracisternally in a dose of 15 μg per rat increased striatal concentrations of the dopamine metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) as well as producing catalepsy. These effects were inhibited by naloxone. Pargyline-induced decreases in striatal DOPAC and HVA were greater in endorphin-treated than in saline-treated animals, supporting the concept that β-endorphin increases striatal dopamine turnover. β-endorphin increased the rate of decline in striatal dopamine concentration following synthesis inhibition with α-methyltyrosine, further suggesting that endorphin increases striatal dopamine turnover. β-endorphin and probenecid interacted competitively to decrease the effects of each other to increase striatal HVA. Naloxone prevented the effect of endorphin to decrease the HVA response to probenecid. Thus, probenecid cannot be used to assess the effects of endorphin on striatal dopamine turnover. If β-endorphin acts presynaptically to decrease dopamine release in striatum, the increases in striatal DOPAC and HVA probably represent a compensatory attempt to increase dopamine synthesis. Although turnover of dopamine to its metabolites is increased, dopamine release may be suppressed by β-endorphin. 相似文献
1000.
Human β-endorphin (15 μg) administered intracisternally increased concentrations of serotonin (5HT) and its metabolite, 5-hydroxyindoleacetic. acid (5-HIAA), in brain stem and hypothalamus and decreased 5-HIAA concentrations in hippocampus. These data are compatible with the hypothesis that β-endorphin increases 5HT turnover in brain stem and hypothalamus and decreases 5HT turnover in hippocampus. β-endorphin increased in brain stem and hypothalamus and decreased in hippocampus the rate of pargyline-induced decline of 5-HIAA. β-endorphin decreased the rate of pargyline-induced accumulation of 5HT in all these brain regions. The probenecid-induced accumulation of 5-HIAA in brain stem was decreased by β-endorphin. These data are compatible with the hypothesis that β-endorphin increases release of 5HT from neurons in brain stem and hypothalamus and decreases release of 5HT from neurons in hippocampus. The data require further a hypothesis that β-endorphin either decreases 5HT reuptake in these three brain regions or increases 5-HIAA egress from brain. 相似文献