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61.
Plant Molecular Biology - 相似文献
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Valuev I. L. Pan A. V. Rozenfeld M. A. Valuev L. I. Plat N. A. 《Applied Biochemistry and Microbiology》2003,39(3):317-320
Copolymers of N,N-diethylacrylamide and N-acryloylphthalimide with a lower critical solution temperature (LCST) were synthesized by radical copolymerization. Polymeric systems with antithrombin activity and an LCST were prepared via a reaction of amino groups of hirudin with phthalimide groups of the copolymers. On increasing hirudin content, the LCST of the polymeric systems increased. The antithrombin activity of polymeric systems obtained by hirudin immobilization on copolymer carriers was inversely related to the content of the copolymer, amounting to 6% of the activity of native hirudin. 相似文献
64.
L V Vanchugova T A Valueva V I Romashkin M A Rozenfel'd L I Valuev 《Biokhimii?a (Moscow, Russia)》1988,53(9):1455-1461
The interaction of highly purified duck egg white ovomucoid with trypsin and chymotrypsin was studied. It was found that the ovomucoid molecule contains two equally effective trypsin-binding sites which, in their turn, comprise lysine residues and one independent chymotrypsin-binding site. The values of inhibition constants were determined and the changes in free energy, enthalpy and entropy during the ovomucoid interaction with trypsin and chymotrypsin were established. It was shown that the inhibitor affinity for the enzymes does not change at low degrees of free amino groups modification. 相似文献
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