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101.
Since the massive bloom in 1988 in the North Sea, the prymnesiophyte flagellate Chrysochromulina polylepis Manton et Parke has been known for its ichtyotoxicity. Laboratory experiments using two different clones of C. polylepis were conducted in a comparative approach. Both clones were nearly similar in size and shape, but differed in their toxicity, as demonstrated by the Artemia bioassay. In order to study the effects of toxic C. polylepis on protozooplankton grazers, grazing experiments were performed with the heterotrophic dinoflagellate Oxyrrhis marina Dujardin as grazer. A first experiment was carried out in order to follow batch culture growth and initial grazing of O. marina when fed toxic or non-toxic clones of C. polylepis. Ingestion of the toxic clone was 27% of ingestion when fed with the non-toxic clone. When O. marina was fed with the toxic clone, vacuoles within O. marina contained fewer food particles per cell and the cells attained slower division rate (58% of the division rate estimated for the non-toxic clone). A second experiment was conducted to determine the grazing and growth response of O. marina as a function of algal food concentration. Profound differences in ingestion, clearance, division and gross growth efficiency of O. marina when fed the two clones of C. polylepis again were apparent. However, even at algal concentrations of 400×103 ml−1, O. marina is not killed by the presence or by the ingestion of toxic C. polylepis, indicating that the toxin deters grazers. In addition to grazing experiments, lipid classes and fatty acids of both algal clones were analysed and compared in order to follow the hypothesis that toxicity of C. polylepis is caused by liposaccharides, lipids, or fatty acids. However, the chemical composition with respect to lipid classes and fatty acids of both clones were quite similar, making an involvement of these substances in the toxicity towards Artemia and O. marina unlikely. 相似文献
102.
Axial Differences in Community Structure of Crenarchaeota and Euryarchaeota in the Highly Compartmentalized Gut of the Soil-Feeding Termite Cubitermes orthognathus 总被引:2,自引:0,他引:2
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Michael W. Friedrich Dirk Schmitt-Wagner Tillmann Lueders Andreas Brune 《Applied microbiology》2001,67(10):4880-4890
Methanogenesis represents an important electron sink reaction in the hindgut of soil-feeding termites. This is the first comprehensive analysis of the archaeal community structure within the highly compartmentalized intestinal tract of a humivorous insect, combining clonal analysis and terminal restriction fragment (T-RF) length polymorphism (T-RFLP) fingerprinting of the archaeal communities in the different gut compartments of Cubitermes orthognathus. We found that the morphological and physicochemical heterogeneity of the gut is reflected in a large phylogenetic diversity and pronounced axial differences in the composition of the archaeal gut microbiota, notably among those clones or ribotypes that could be assigned to methanogenic taxa. Comparative analysis of the relative frequencies of different archaeal lineages among the small-subunit rRNA gene (SSU rDNA) clones and their corresponding T-RF indicated that the archaeal community in the anterior, extremely alkaline hindgut compartment (P1) consists mainly of members of the Methanosarcinaceae, whereas Methanobacteriaceae and Methanomicrobiales predominate in the subsequent, more posterior compartments (P3/4a and P4b). The relative abundance of Thermoplasmales increased towards the rectum (P5). SSU rDNA sequences representing Crenarchaeota, which have not yet been reported to occur in the intestinal tracts of arthropods, were detected in all gut sections. We discuss how the spatial distribution of methanogenic populations may be linked to axial heterogeneity in the physicochemical gut conditions and to functional adaptations to their respective ecological niches. 相似文献
103.
Studies in acidified as well as in naturally base-poor areas have recently revealed that availability of extra calcium-rich food items is an important component of habitat quality affecting breeding performance in several bird species. However, these mostly short-term studies have provided equivocal results concerning the exact consequences of calcium shortage on different species in different regions. We studied the effect of calcium availability on reproduction of the Pied Flycatcher Ficedula hypoleuca breeding in pine forests in Estonia, NE Europe, over a period of 4 years. Experimental pairs were provided with supplementary calcium-rich material when breeding, while control pairs were left unsupplemented. Experimental females laid larger eggs and their nestlings had longer tarsi than those of controls. Moreover, the mass and condition of females tending larger than average clutches were increased by calcium-supplementation. Our results provide the first experimental evidence that calcium availability may affect the overall cost of reproduction in free-living passerines. We compared these results with similar data for the Great Tit Parus major , collected from the same area during the same study period. Great Tits responded to low calcium availability mainly by restrained reproductive behaviour and reduced breeding success, while Pied Flycatchers invested significantly more in current reproductive effort despite the increased cost of reproduction. Thus, the effects of calcium deficiency on birds seem to be species-specific or population-specific. This partly explains discrepancies between the results of earlier studies. 相似文献
104.
M Geiser H P D?ring J W?stemeyer U Behrens E Tillmann P Starlinger 《Nucleic acids research》1980,8(24):6175-6188
A cDNA clone for maize endosperm sucrose synthetase of 62o nucleotide pairs length was obtained by cloning double stranded DNA obtained from the total maize endosperm poly(A) RNA in pBR322, and identifying the appropriate clone by hybrid-promoter translation. In Southern blotting to genomic BamHI-digested DNA, a single band only of approximately 20 Kb lights up, indicating that the sucrose synthetase gene is unique, or that closely linked copies are located on this DNA fragment. 相似文献
105.
Jo?o?Augusto?Alves?Meira-NetoEmail authorView authors OrcID profile Maria?Carolina?Nunes?Alves?da?Silva Gláucia?Soares?Tolentino Markus?Gastauer Tillmann?Buttschardt Florian?Ulm Cristina?Máguas 《Biological invasions》2018,20(6):1567-1575
Australian species of the genus Acacia are amongst the most invasive trees. As nitrogen fixers, they are able to invade oligotrophic ecosystems and alter ecosystem functioning to their benefit. We aimed to answer three questions: How does early Acacia invasion influence nitrogen and light in a sandy savanna? How does early Acacia invasion impact biodiversity? Does early invasion alter ecosystem functioning towards the dominance of Acacia? We analyzed (using generalized linear mixed models and richness estimators) paired plots focused on plants of Acacia mangium (Fabaceae) and plants of Marcetia taxifolia (Melastomataceae) by taking hemispherical photos and sampling plants, leaves and soil for measurements of light, richness, leaf nitrogen, leaf δ15N, soil nitrogen and soil coarse sand. The results suggest that early Acacia invasion alters the control of soil and of leaf nitrogen and increases shading, enabling a much wider range of light variation. The δ15N results suggest that the nitrogen taken up by Acacia is transferred to neighboring plants and influences the light environment, suggesting facilitation. The enrichment of plant species observed during early Acacia invasion is consistent with the wider range of light variation, but the forecasted leaf nitrogen conditions during the established phase of Acacia invasion might cause loss of light-demanding species because of increased shading. If early Acacia invasion turns into an established phase with highly increased shading, Acacia seedlings might be favored and ecosystem functioning might change towards its dominance. 相似文献
106.
The purpose of this experiment was to determine whether diploid and triploid brook charr, Salvelinus fontinalis, differ in their critical thermal maxima (CTM). Two age classes were tested (underyearlings, having average weight of 25 g, and yearlings, having average weight of 668 g) at two rates of temperature increase (2° C h-1 and 15° C h-1). No effect of ploidy on CTM was found. Fish exposed to the faster rate of temperature increase had higher CTM values than those exposed to the slower rate (underyearlings: 29.5 ± 0.1° C versus 29.1 ± 0.1° C in one trial and 29.8 ± 0.1° C versus 28.3 ± 0.1° C in a second trial; yearlings: 29.3 ± 0.1° C versus 27.7 ± 0.1° C in two trials, p < 0.001 in all cases). Underyearlings had higher CTM values than yearlings (29.2 ± 0.1° C versus 28.5 ± 0.1° C, p < 0.05). Female yearlings, which were immature, had higher CTM values than males, which had previously matured as one-year-olds (28.8 ± 0.1° C versus 28.3 ± 0.1° C, p < 0.001). 相似文献
107.
Silvia Gschwendtner Marion Engel Tillmann Lueders Franz Buegger Michael Schloter 《Plant and Soil》2016,409(1-2):203-216
Background and aims
Variation in fire intensity within an ecosystem is likely to moderate fire effects on plant and soil properties. We tested the effect of fire intensity on grassland biomass, soil microbial biomass, and soil nutrients. Additional tests determined plant-microbe, plant-nutrient, and microbe-nutrient associations.Methods
A replicated field experiment produced a fire intensity gradient. We measured plant and soil microbial biomasses at peak plant productivity the first growing season after fire. We concurrently measured flux in 11 soil nutrients and soil moisture.Results
Fire intensity positively affected soil nitrogen, phosphorus (P), and zinc but did not appreciably affect plant biomass, microbial biomass, and other soil nutrients. Plant biomass was seemingly (co-)limited by boron, manganese, and P. Microbial biomass was (co-)limited mainly by P and also iron.Conclusions
In the Northern Great Plains, plant and soil microbial biomasses were limited mainly by P and some micronutrients. Fire intensity affected soil nutrients, however, pulsed P (due to fire) did not result in appreciable fire intensity effects on plant and microbial biomasses. Variable responses in plant productivity to fire are common and indicate the complexity of factors that regulate plant production after fire.108.
Amphidoma is an old though poorly studied thecate dinophyte that has attracted attention recently as a potential producer of azaspiracids (AZA), a group of lipophilic phycotoxins. A new species, Amphidoma parvula, sp. nov. is described from the South Atlantic shelf of Argentina. With a Kofoidean thecal plate pattern Po, cp, X, 6′, 6′′, 6C, 5S, 6′′′, 2′′′′, the cultivated strain H-1E9 (from which the type material of Am. parvula, sp. nov. was prepared) shared the characteristic plate arrangement of Amphidoma each with six apical, precingular and postcingular plates. Amphidoma parvula, sp. nov. differs from other species of Amphidoma by a characteristic combination of small size (10.7–13.6 µm in length), ovoid shape, high length ratio between epitheca and hypotheca, and small length ratio between apical and precingular plates. Other morphological details, such as the number and arrangement of sulcal plates and the fine structure of the apical pore complex support the close relationship between Amphidoma and the other known genus of Amphidomataceae, Azadinium. However, Am. parvula, sp. nov. lacks a ventral pore, a characteristically structured pore found in all contemporary electron microscopy studies of Amphidoma and Azadinium. As inferred from liquid chromatography coupled with tandem mass spectrometry, Am. parvula, sp. nov. did not produce AZA in measurable amounts. Molecular phylogenetics confirmed the systematic placement of Am. parvula, sp. nov. in Amphidoma (as sister species of Amphidoma languida) and the Amphidomataceae. The results of this study have improved the knowledge of Amphidomataceae biodiversity. 相似文献
109.
Dinoflagellates play important roles in marine food webs, both as primary producers and as heterotrophic consumers. They produce spectacular phenomena like marine bioluminescence and red tides, and are major components of coral reefs. Members of the group can also synthesize some of the most toxic biogenic compounds currently known. Dinoflagellate's nuclei are extremely large and biochemically unique, while mitochondrial genomes are very small. The plastid diversity of dinoflagellates is also unique: only about half of the species in the group are photosynthetic, but the origin of their plastids is often different. As a consequence dinoflagellate plastids have very diverse physiological and biochemical characteristics. Some of the most complex morphological constructs known in unicellular organisms are found in this group. These include ?harpoons“, used to capture prey, and ?eyes“, that contain substructures reminiscent of lenses, corneas and retinas. 相似文献
110.