Spring-wells (lowland springs, “fontanili”) are elements of Po Plain (Northern Italy) with ecological and historical importance. There, spring-water flows naturally and rises to the surface at a relatively constant temperature, generating unique ecosystems dependent on the groundwater outflow. Despite their importance, they are endangered by degradation processes as the expansion of urban areas and/or the intensification of agriculture, very marked in Po Plain. This research describes four spring-wells of Po Plain from a botanical and ecological perspective through phytosociological relevés and different ecological indexes. Water chemical-physical features are also considered (pH, temperature and ammonium ion, nitrite, nitrate, orthophosphate, chloride and organic matters contents). Plant communities of the spring-well beds show a low number of species (5.8?±?2.9) but also no exotic species while the vegetation of the banks has a high number of species (32.4?±?9.8) but several of them are exotic/ornamental. The low value of the Ecological Index of Maturity of the vegetation of the banks (EIM?=?4.4?±?1.5) indicate disturbances, however moderate compared to the surrounding corn fields (EIM?~?0). All the water samples has high ammonium content (>?0.50 ppm), the water of the spring-well B results the most polluted and both algae and Callitriche obtusangula (rare native species) grow largely in it, while spring-well C has less phosphates and more nitrite and is marked by Equisetum telmateia. Management proposals to protect and enhance the studied spring-wells, as the gradual removal of exotic species and the planting of native species, are discussed.
Summary This study provides evidence thatGigaspora margarita replicates its nuclear DNA, even in the absence of a host plant. Three experimental approaches were used: (i) static cytofluorimetry to quantify the DNA content, (ii) pulse treatments with bromodeoxyuridine (BrdU), which is an analogue of thymidine, to reveal nuclei undergoing DNA synthesis, and (iii) ultrastructural observations to study changes in chromatin morphology during the fungal cell cycle. A slight second peak of approximately twice the value of a major peak was found by cytofluorimetry, showing that a small number of nuclei had entered in cycle during in vitro development. Nuclei which had incorporated BrdU were observed after pulses of 24 h; nuclei with condensed chromatin were also apparent at this time. The results demonstrate thatG. margarita has all the metabolic pathways needed to replicate its nuclear DNA even in the absence of the host, suggesting that more complex mechanisms inhibit the extended growth in vitro of arbuscular mycorrhizal fungi.Abbreviations AM-fungi
arbuscular mycorrhizal fungi
- A.U.
arbitrary units
- BrdU
5-bromo-2-deoxyuridine
- DAPI
4,6-diamidino-2-phenylindole
- UV
ultraviolet light 相似文献
Lake Baikal is considered as a unique place to study evolution. In this review, we report on recent data on the evolution of endemic freshwater sponges of this ancient lake. Nucleotide sequence data support the idea that these sponges are of monophyletic origin and evolved from Spongillidae. Baikalian sponges form the dominating biomass in the benthos of the lake. Data on the expression of the biomarker heat shock protein 70, revealed that the endemic sponge species of Lake Baikal are useful as bioindicators to assess the anthropogenic impact on the lake. 相似文献
Cuprizone affects the liver of treated mice in a random manner, causing no appreciable change in some cases and inducing the formation of megamito-chondria with altered properties in others. Lack of a full appreciation of this variability may be at the origin of some discrepancies in published work dealing with the properties of cuprizone mouse liver mitochondria (CMLM). CMLM from fully affected livers were remarkably labile and difficult to isolate in a coupled state by homogenization and centrifugation techniques. The integral respiratory chain proteins of CMLM were functionally normal, with the exception of succinic dehydrogenase which showed considerable inhibition. Coupled morphological and functional analysis provided evidence that these properties were independent of CMLM size, a matter which had remained doubtful thus far and bears on the validity of literature reports.Abbreviations MLM
mouse liver mitochondria
- CMLM
cuprizone mouse liver mitochondria
- PMS
phenazine methosulfate
- Mops
4-morphoinopropanesulfonic acid
- TMPD
tetramethyl-p-phenylenediamine
- AA
Antimycin A
- RCR
respiratory control ratio
- BSA
bovine serum albumin 相似文献
Toxicity is widespread among living organisms, and evolves as a multimodal phenotype. Part of this phenotype is the ability to avoid self‐intoxication (autoresistance). Evolving toxin resistance can involve fitness tradeoffs, so autoresistance is often expected to evolve gradually and in tandem with toxicity, resulting in a correlation between the degrees of toxicity and autoresistance among toxic populations. We investigate this correlation in Phyllobates poison frogs, notorious for secreting batrachotoxin (BTX), a potent neurotoxin that targets sodium channels, using ancestral sequence reconstructions of BTX‐sensing areas of the muscular voltage‐gated sodium channel. Reconstructions suggest that BTX resistance arose at the root of Phyllobates, coinciding with the evolution of BTX secretion. After this event, little or no further evolution of autoresistance seems to have occurred, despite large increases in toxicity throughout the history of these frogs. Our results, therefore, provide no evidence in favor of an evolutionary correlation between toxicity and autoresistance, which conflicts with previous work. Future research on the functional costs and benefits of mutations putatively involved in BTX resistance, as well as their prevalence in natural populations, should shed light on the evolutionary mechanisms driving the relationship between toxicity and autoresistance in Phyllobates frogs. 相似文献
Molecular Biology Reports - Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related death mainly due to its high metastatic rate. Impairment of redox homeostasis mechanisms... 相似文献
Inert dusts are an early form of insecticide which is still in use. One of the most common inert dusts is volcanic ash. In order to study the reaction of rangeland grasshoppers, Dichroplus vittigerum (Acrididae) and a katydid, Burgilis mendosensis (Phaneropteridae), to the presence of volcanic ash in their food sources and how this reaction changed as a function of time, we conducted paired preference tests between clean leaves of their preferred host plant and leaves exposed to volcanic ash of different grain size. The behavioral response was measured as the rating on the Thurstonian preference scale of leaves with ash in relation to clean leaves. The results showed that the avoidance of volcanic ash increased as a function of time in both species. Both species studied are occasionally exposed to volcanic activity, and come from an area in which a volcanic eruption had recently occurred. As their populations did not decrease after the ash fall, we propose that some behavioral responses such as avoidance of places with ash, works as tolerance mechanism to inert dusts exposure. 相似文献
Cadmium represents one of the most toxic pollutants in plant ecosystems: at high concentrations it can cause severe effects, such as plant growth inhibition, decrease in photosynthesis and changes in plant basal metabolism. Changes in pigments’ content, RubisCO large subunit, and D1 protein indicated a severe reduction in photosynthetic efficiency. Furthermore, the decrease of nitrate reductase activity and changes in free amino acids levels show a general stress condition of nitrogen assimilation. Cadmium increased the activities of ROS scavenging enzymes; among these, ascorbate peroxidase rate was the most noticeably increased. It is worth noting that glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.64), showed changes in both activities and occurrence during cadmium stress. Interestingly, our data suggest that G6PDH would modulate redox homeostasis under metal exposure, and possibly satisfy the increased request of reductants to counteract the oxidative burst induced by cadmium. Therefore, the results suggest that APX and G6PDH may play a pivotal role to counteract the oxidative stress induced by cadmium in young barley plants. 相似文献