全文获取类型
收费全文 | 2725篇 |
免费 | 182篇 |
出版年
2024年 | 3篇 |
2023年 | 16篇 |
2022年 | 34篇 |
2021年 | 93篇 |
2020年 | 46篇 |
2019年 | 69篇 |
2018年 | 89篇 |
2017年 | 77篇 |
2016年 | 109篇 |
2015年 | 162篇 |
2014年 | 216篇 |
2013年 | 231篇 |
2012年 | 309篇 |
2011年 | 260篇 |
2010年 | 159篇 |
2009年 | 141篇 |
2008年 | 143篇 |
2007年 | 148篇 |
2006年 | 126篇 |
2005年 | 109篇 |
2004年 | 96篇 |
2003年 | 80篇 |
2002年 | 76篇 |
2001年 | 14篇 |
2000年 | 10篇 |
1999年 | 18篇 |
1998年 | 10篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1994年 | 8篇 |
1993年 | 12篇 |
1992年 | 2篇 |
1991年 | 3篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1982年 | 2篇 |
1980年 | 3篇 |
1979年 | 1篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有2907条查询结果,搜索用时 15 毫秒
11.
Matthew J. Hansen Paolo Domenici Palina Bartashevich Alicia Burns Jens Krause 《Biological reviews of the Cambridge Philosophical Society》2023,98(5):1687-1711
Group-hunting is ubiquitous across animal taxa and has received considerable attention in the context of its functions. By contrast much less is known about the mechanisms by which grouping predators hunt their prey. This is primarily due to a lack of experimental manipulation alongside logistical difficulties quantifying the behaviour of multiple predators at high spatiotemporal resolution as they search, select, and capture wild prey. However, the use of new remote-sensing technologies and a broadening of the focal taxa beyond apex predators provides researchers with a great opportunity to discern accurately how multiple predators hunt together and not just whether doing so provides hunters with a per capita benefit. We incorporate many ideas from collective behaviour and locomotion throughout this review to make testable predictions for future researchers and pay particular attention to the role that computer simulation can play in a feedback loop with empirical data collection. Our review of the literature showed that the breadth of predator:prey size ratios among the taxa that can be considered to hunt as a group is very large (<100 to >102). We therefore synthesised the literature with respect to these predator:prey ratios and found that they promoted different hunting mechanisms. Additionally, these different hunting mechanisms are also related to particular stages of the hunt (search, selection, capture) and thus we structure our review in accordance with these two factors (stage of the hunt and predator:prey size ratio). We identify several novel group-hunting mechanisms which are largely untested, particularly under field conditions, and we also highlight a range of potential study organisms that are amenable to experimental testing of these mechanisms in connection with tracking technology. We believe that a combination of new hypotheses, study systems and methodological approaches should help push the field of group-hunting in new directions. 相似文献
12.
Valentina Sora Adrian Otamendi Laspiur Kristine Degn Matteo Arnaudi Mattia Utichi Ludovica Beltrame Dayana De Menezes Matteo Orlandi Ulrik Kristoffer Stoltze Olga Rigina Peter Wad Sackett Karin Wadt Kjeld Schmiegelow Matteo Tiberti Elena Papaleo 《Protein science : a publication of the Protein Society》2023,32(1):e4527
Reliable prediction of free energy changes upon amino acid substitutions (ΔΔGs) is crucial to investigate their impact on protein stability and protein–protein interaction. Advances in experimental mutational scans allow high-throughput studies thanks to multiplex techniques. On the other hand, genomics initiatives provide a large amount of data on disease-related variants that can benefit from analyses with structure-based methods. Therefore, the computational field should keep the same pace and provide new tools for fast and accurate high-throughput ΔΔG calculations. In this context, the Rosetta modeling suite implements effective approaches to predict folding/unfolding ΔΔGs in a protein monomer upon amino acid substitutions and calculate the changes in binding free energy in protein complexes. However, their application can be challenging to users without extensive experience with Rosetta. Furthermore, Rosetta protocols for ΔΔG prediction are designed considering one variant at a time, making the setup of high-throughput screenings cumbersome. For these reasons, we devised RosettaDDGPrediction, a customizable Python wrapper designed to run free energy calculations on a set of amino acid substitutions using Rosetta protocols with little intervention from the user. Moreover, RosettaDDGPrediction assists with checking completed runs and aggregates raw data for multiple variants, as well as generates publication-ready graphics. We showed the potential of the tool in four case studies, including variants of uncertain significance in childhood cancer, proteins with known experimental unfolding ΔΔGs values, interactions between target proteins and disordered motifs, and phosphomimetics. RosettaDDGPrediction is available, free of charge and under GNU General Public License v3.0, at https://github.com/ELELAB/RosettaDDGPrediction . 相似文献
13.
Marina S. Trofimova Inna M. Zhestkova Valentina P. Kholodova Igor M. Andreev Evgeny M. Sorokin Anna G. Kruglova Vladimir V. Kuznetsov 《Physiologia plantarum》2003,118(2):232-239
The osmotic water permeability ( P os ) of cell membranes isolated from leaves of 40-, 50- and 60-day-old Mesembryanthemum crystallinum plants was estimated by measuring light-scattering kinetics using stopped-flow spectrophotometry. The measurements were performed on the plasma membrane (PM), purified tonoplast (TP), and TP-enriched vesicles. The PM and TP-enriched vesicles were obtained by partitioning the microsomal fraction in an aqueous polymer two-phase system, whereas the purified TP vesicles were prepared by microsomal vesicle flotation on a sucrose cushion. The P os of isolated membranes declined with plant age. The kinetic experiments showed that there was no difference between the P os of the PM and TP isolated from plants of all ages. A 24-h exposure of plants to 400 m M NaCl caused a decline in the P os as well. These findings suggest that, during M. crystallinum transition to CAM, which was induced by plant ageing or salinity, plant osmoregulatory responses included changes in the P os of the leaf-cell membranes. These variations in the P os are discussed in the context of adaptive mechanisms responsible for the maintenance of the water balance in the common ice plant. 相似文献
14.
Fine structure and functional role of the coverings of the eggs inMesostoma ehrenbergii (Focke) (Turbellaria,Neorhabdocoela) 总被引:1,自引:0,他引:1
Summary European populations of the turbellarianMesostoma ehrenbergii can produce two types of eggs according to a seasonal cycle. Subitaneous eggs (S eggs) are produced throughout the warm season and develop rapidly inside the parental uteri. They are meant to ensure a fast increase in the number of worms. Dormant eggs (D eggs) are produced during the warm season and are usually deposited in water at the death of the parent. They can hatch at the beginning of the next warm season and are, therefore, meant to ensure the survival of the population throughout the cold season, when adult worms are no longer present.S eggs are bounded by a thin, translucent covering which has a loose structure and is mainly proteinaceous in composition. These characteristics account for the functional role played by the S egg covering during embryonic development. The covering has, in fact, to be permeable to parental nutrients, and to keep pace, by stretching, with the enlargement of the embryo. By contrast, D eggs are bounded by a thick shell, presumably composed of a sclerotin, the precursors of which (polyphenols) are synthesized together with yolk in the vitelline cells. The shell has a smooth surface devoid of pores and has the function of isolating the developing D egg from the external environment.Supported by a grant from the Consiglio Nazionale della RicercheThe authors wish to thank Dr. P. Roach for his help in translating the paper 相似文献
15.
Multiple enzymic lesions in obligate methanotrophic bacteria 总被引:1,自引:0,他引:1
16.
Summary The antitumour activity of C. parvum and its relationship to spleen weight increase have been analysed in a tumour model using syngeneic M4 fibrosarcoma cells. It was found that treatment of C. parvum with low concentrations of periodate (2 mM) produced a minor decrease in the ability to abrogate tumour growth, following intratumour injection, although the capacity to increase spleen weight after IP injection was abolished. Higher concentrations of periodate (20 mM) produced complete inactivation of the bacteria. A phenol extract of C. parvum was weakly active in the intratumour test but amounts up to 1 mg injected IP were unable to increase the spleen weight. The data support the hypothesis that a surface carbohydrate is responsible for the increase in spleen weight. It is suggested that the antitumour activity may reside in a molecule that also reacts with periodate but at a slower rate, perhaps because of a more complex structure or a different location in the cell, for instance in the cytoplasm. The phenol extract was soluble in chloroform and at least two major components were glycolipids, so it is conceivable that a molecule of this type, which could be degraded by periodate, might be responsible for the antitumour activity of C. parvum. The precise biochemical nature of the active component is, as yet, undetermined. 相似文献
17.
Mariarosaria Bucci Anna Cantalupo Valentina Vellecco Elisabetta Panza Maria Chiara Monti Angela Zampella Angela Ianaro Giuseppe Cirino 《PloS one》2013,8(3)
Here we have characterized perthamide C, a cyclopeptide from a Solomon Lithistid sponge Theonella swinhoei, which displays an anti-inflammatory/immunomodulatory activity. The study has been performed using the carragenan-induced mouse paw edema that displays an early (0–6 h) and a late phase (24–96 h). Perthamide C significantly inhibits neutrophils infiltration in tissue both in the early and late phases. This effect was coupled to a reduced expression of the endothelial nitric oxide synthase (eNOS) in the early phase while cyclooxygenase-1 and 2 (COX-1, COX-2), and inducible NOS (iNOS) expression were unaffected. In the late phase perthamide C reduced expression of both NOS isoforms without affecting COXs expression. This peculiar selectivity toward the two enzymes deputed to produce NO lead us to investigate on a possible action of perthamide C on lymphocytes infiltration and activation. We found that perthamide C inhibited the proliferation of peripheral lymphocytes, and that this effect was secondary to its metabolic activation in vivo. Indeed, in vitro perthamide C did not inhibit proliferation as opposite to its metabolite perthamide H.In conclusion, perthamide C selectively interferes with NO generation triggered by either eNOS or iNOS without affecting either COX-1 or COX-2. This in turn leads to modulation of the inflammatory response through a reduction of vascular permeability, neutrophil infiltration as well as lymphocyte proliferation. 相似文献
18.
19.
Bertero Valentina Goytia Beznec A. Faccio P. Auteri M. Arteaga M. Bonafede M. Bossio E. 《In vitro cellular & developmental biology. Plant》2020,56(6):915-919
In Vitro Cellular & Developmental Biology - Plant - In vitro culture has been recognized as a potential plant clonal propagation tool for a broad variety of commercial, ornamental, and... 相似文献
20.
Ratajczak Mariusz Z. Adamiak Mateusz Bujko Kamila Thapa Arjun Pensato Valentina Kucia Magda Ratajczak Janina Ulrich Henning 《Purinergic signalling》2020,16(2):153-166
Purinergic Signalling - Bone marrow (BM) as an active hematopoietic organ is highly sensitive to changes in body microenvironments and responds to external physical stimuli from the surrounding... 相似文献