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111.
The development of the central nervous system can be divided into a number of phases, each of which can be subject of genetic or epigenetic alterations that may originate particular developmental disorders. In recent years, much progress has been made in elucidating the molecular and cellular mechanisms by which the vertebrate forebrain develops. Therefore, our understanding of major developmental brain disorders such as cortical malformations and neuronal migration disorders has significantly increased. In this review, we will describe the major stages in forebrain morphogenesis and regionalization, with special emphasis on developmental molecular mechanisms derailing telencephalic development with subsequent damage to cortical function. Because animal models, mainly mouse, have been fundamental for this progress, we will also describe some characteristic mouse models that have been capital to explore these molecular mechanisms of malformative diseases of the human brain. Although most of the genes involved in the regulation of basic developmental processes are conserved among vertebrates, the extrapolation of mouse data to corresponding gene expression and function in humans needs careful individual analysis in each functional system. 相似文献
112.
JoséR. Valdes Santurio Esther González Porto 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1996,682(2):364
A sensitive high-performance liquid chromatographic method for determination of intact glibenclamide in human plasma has been developed. Sample clean-up prior to chromatographic analysis was accomplished by extraction of the drug using a solid-phase RP-8 or RP-18 cartridge instead of the conventional liquid-liquid extraction methods described. For the separation of the drug from the endogenous components a reversed-phase column (LiChrosorb RP-8) of 5 μm particle size and 250×4 mm I.D., together with a mobile phase consisting of acetonitrile-12 μM perchloric acid (47:53) was selected. The method employs progesterone as an internal standard, and a reversed-phase column combined with UV detection of the drug at 230 nm. The detector response was linear up to the concentration of 400 ng/ml and the average recovery was 100.36%. The sensitivity of the method was 5 ng/ml. 相似文献
113.
The external morphology and anatomy of the opisthobranch gastropodsMiamira
sinuata (van Hasselt, 1824) and Orodoris miamiranaBergh, 1875, the type
species of the genera Miamira Bergh, 1875and Orodoris Bergh, 1875, and
their phylogenetic relationshipsare studied. The phylogeny obtained supports the placement ofM. sinuata and O. miamirana in the genus Ceratosoma J. E. Gray,
1850.Therefore, Miamira and Orodoris become synonyms of the seniorvalid
name Ceratosoma. In addition, the family name MiamiridaeBergh, 1891, based on Miamira, is newly recognized as a synonymof Chromodorididae Bergh, 1891. Ceratosoma sinuata and C. miamirana are more closely relatedto the
highly derived Ceratosoma alleni than to other membersof the genus. C. miamirana appears to present reversal to theplesiomorphic state in the body shape and has secondarily
lostits mantle glands. (Received 5 January 1998; accepted 23 April 1998) 相似文献
114.
Valdes Labrada Guadalupe Montserrat Nemati Mehdi 《Bioprocess and biosystems engineering》2018,41(11):1635-1649
Bioprocess and Biosystems Engineering - Waters contaminated with naphthenic acids (NAs) and associated tailings are one of the major environmental challenges associated with the processing of oil... 相似文献
115.
W E Bentley R D Madurawe R T Gill M Shiloach T E Chase T R Pulliam-Holoman J J Valdes 《Journal of industrial microbiology & biotechnology》1998,21(6):275-282
Recombinant E. coli clones expressing a 50-kDa poly-histidine tail tagged antibody fragment against botulinum toxin (bt-Fab) were initially screened
for yield and binding affinity. One clone was selected for bioprocess development. The selected bt-Fab vector was induced
by addition of IPTG and the protein was targeted to the periplasm by inclusion of a pelB leader sequence. A histidine6 affinity ligand at the heavy chain C-terminus facilitated single-step purification by immobilized metal-affinity chromatography
(IMAC). Notably, the effects of post-induction temperature on bt-Fab expression and downstream purification were evaluated.
Our results demonstrated that fermentation conditions interfered with purification on the IMAC column at 37°C. Protease analysis
by gelatin polyacrylamide gel electrophoresis (GPAGE) indicated the presence of a membrane-bound ∼39 kDa protease activity
shortly after induction. The appearance of the protease activity was inversely correlated with the bt-Fab yield. The protease
was purified and was shown to degrade bt-Fab. A simple kinetic model was developed describing temporal regulation of protease
and bt-Fab degradation. Partially degraded bt-Fab was unrecoverable by IMAC, presumably due to the loss of the His6 affinity ligand. The amount of purified bt-Fab obtained per liter of fermentation broth was typically ∼1 mg.
Received 18 August 1997/ Accepted in revised form 4 October 1998 相似文献
116.
V. Dehant D. Asael R. M. Baland B. K. Baludikay J. Beghin J. Belza M. Beuthe D. Breuer S. Chernonozhkin Ph. Claeys Y. Cornet L. Cornet A. Coyette V. Debaille C. Delvigne M. H. Deproost N. De WInter C. Duchemin F. El Atrassi C. Fran?ois J. De Keyser C. Gillmann E. Gloesener S. Goderis Y. Hidaka D. H?ning M. Huber G. Hublet E. J. Javaux ?. Karatekin J. Kodolanyi L. Lobo Revilla L. Maes R. Maggiolo N. Mattielli M. Maurice S. McKibbin A. Morschhauser W. Neumann L. Noack L. B. S. Pham L. Pittarello A. C. Plesa A. Rivoldini S. Robert P. Rosenblatt T. Spohn J. -Y. Storme N. Tosi A. Trinh M. Valdes A. C. Vandaele F. Vanhaecke T. Van Hoolst N. Van Roosbroek V. Wilquet M. Yseboodt the Planet TOPERS group 《Origins of life and evolution of the biosphere》2016,46(4):369-384
117.
Metabolomic Profiling of Long‐Term Weight Change: Role of Oxidative Stress and Urate Levels in Weight Gain 下载免费PDF全文
118.
Annie W.C. Kung Su-Mei Xiao Stacey Cherny Yi Gao Kam S. Lau Jian-min Liu Min-Jia Zhang Jin-wei He Wia-bo Xia Shu-li He David Karasik L. Adrienne Cupples Serkalem Demissie Unnur Styrkarsdottir Gunnar Sigurdsson Unnur Thorsteinsdottir Kari Stefansson J. Brent Richards Guangju Zhai Ana Valdes Pak C. Sham 《American journal of human genetics》2010,86(2):229-239
Bone mineral density (BMD), a diagnostic parameter for osteoporosis and a clinical predictor of fracture, is a polygenic trait with high heritability. To identify genetic variants that influence BMD in different ethnic groups, we performed a genome-wide association study (GWAS) on 800 unrelated Southern Chinese women with extreme BMD and carried out follow-up replication studies in six independent study populations of European descent and Asian populations including 18,098 subjects. In the meta-analysis, rs2273061 of the Jagged1 (JAG1) gene was associated with high BMD (p = 5.27 × 10−8 for lumbar spine [LS] and p = 4.15 × 10−5 for femoral neck [FN], n = 18,898). This SNP was further found to be associated with the low risk of osteoporotic fracture (p = 0.009, OR = 0.7, 95% CI 0.57–0.93, n = 1881). Region-wide and haplotype analysis showed that the strongest association evidence was from the linkage disequilibrium block 5, which included rs2273061 of the JAG1 gene (p = 8.52 × 10−9 for LS and 3.47 × 10−5 at FN). To assess the function of identified variants, an electrophoretic mobility shift assay demonstrated the binding of c-Myc to the “G” but not “A” allele of rs2273061. A mRNA expression study in both human bone-derived cells and peripheral blood mononuclear cells confirmed association of the high BMD-related allele G of rs2273061 with higher JAG1 expression. Our results identify the JAG1 gene as a candidate for BMD regulation in different ethnic groups, and it is a potential key factor for fracture pathogenesis. 相似文献
119.
Mapping genetic loci that determine leukocyte telomere length in a large sample of unselected female sibling pairs 下载免费PDF全文
Andrew T Aviv A Falchi M Surdulescu GL Gardner JP Lu X Kimura M Kato BS Valdes AM Spector TD 《American journal of human genetics》2006,78(3):480-486
Telomeres play a central role in cellular senescence and cancer pathobiology and are associated with age-related diseases such as atherosclerosis and dementia. Telomere length varies between individuals of the same age, is influenced by DNA-damaging factors such as oxidative stress, and is heritable. We performed a quantitative-trait linkage analysis using an approximate 10-cM genomewide map for mean leukocyte terminal-restriction fragment (TRF) lengths measured by Southern blotting, in 2,050 unselected women aged 18-80 years, comprising 1,025 complete dizygotic twin pairs. Heritability of mean batch-adjusted TRF was 36% (95% confidence interval [CI] 18%-48%), with a large common environmental effect of 49% (95% CI 40%-58%). Significant linkage was observed on chromosome 14 (LOD 3.9) at 14q23.2, and suggestive linkage at 10q26.13 (LOD 2.4) and 3p26.1 (LOD 2.7). This is the first report of loci, mapped in a sample of healthy individuals, that influence mean telomere variation in humans. 相似文献
120.
Magri F Gabellieri E Busconi L Guazzoni V Cravello L Valdes V Sorrentino AR Chytiris S Ferrari E 《Life sciences》2006,78(22):2625-2632
Randomized clinical trials have not shown long-term benefit of postmenopausal hormone replacement therapy (PHT) nor have they shown conclusively that the harmful consequences outweighs the benefits of the treatment. Rather, it is possible that an individualized hormone replacement therapy in questionably clinically healthy postmenopausal women may lead to different results than randomized trials. DESIGN: In this cross-sectional study we evaluated anthropometric parameters, body composition, serum lipids, blood pressure, heart rate variability (HRV) and neurocognitive functions in 39 healthy postmenopausal women PHT users or not users (n=13, age 53.0+/-3.3 and n=26, age=53.3+/-5.0 SD, respectively) as well as in 27 younger controls (ages=33.3+/-7.1). RESULTS: Demographic parameters were similar in women PHT users and not users. Postmenopausal women showed a significantly increase of body mass index (BMI) as well as of waist circumference, compared to younger controls, but in PHT users the values of fat free mass were intermediate between the ones of not treated and younger women. The study of HRV showed a reduction in low frequency (LF) component (sympathetic modulation) during the day, and a reduction in high frequency (HF) component (parasympathetic modulation), particularly in postmenopausal women without PHT. PHT users were characterized by autonomic parameters intermediate between younger controls and age-matched women without PHT. CONCLUSIONS: The impact of PHT on the age-dependent changes of anthropometric features and body composition seems to be modest but positive. Furthermore, PHT seems to play a positive role on the autonomic modulation of cardiac function, through a shift of LF/HF ratio values towards those of young controls. 相似文献