排序方式: 共有150条查询结果,搜索用时 15 毫秒
31.
Emmanuel K. Nénonéné Fatiha Radja Martin Carli Nico M. van Gelder Soheila Afkhami-Dastjerdian Tomás A. Reader 《Neurochemical research》1996,21(2):167-176
The binding of tritiated 8-hydroxy-2-(di-n-propyl-amino)tetralin, or [3H]8-OH-DPAT, to membranes from rat cerebral cortex and hippocampus could be inhibited by serotonin (5-HT) and buspirone, and
by the 5-HT antagonists propranolol, NAN-190, pindolol, pindobind-5-HT1A, WAY100135, spiperone and ritanserin. All competition curves, except for ritanserin, best fitted a two-site model. In vitro
treatment of the membranes withN-ethylmaleimide (NEM), to alkylate sulfhydryl groups, caused dose-dependent decreases of binding; the inhibition curves were
biphasic, and the effects irreversible. Reduction of disulfide bonds withl-dithiothreitol (L-DTT) also decreased binding, but in a monophasic way; these effects were fully reversible in cortex, but only partially reversible
in hippocampus. In the latter region, but not in cerebral cortex, previous occupancy by [3H]8-OH-DPAT partially protected binding from the effects of bothL-DTT and NEM, suggesting that the thiol groups in the receptor recognition site(s) of this brain region are readily accessible.
The binding characteristics were examined with the aid of saturation curves, carried out with increasing concentrations, up
to 140 nM, of [3H]8-OH-DPAT. The saturation data were suggestive of a two-site receptor model incorporating a high-affinity site (Kh of 0.3–0.5 nM) corresponding to the 5-HT1A receptor, and a low-affinity site (Kl ofca 25 nM). After in vivo alkylations, carried out by treating rats withN-ethoxycarbonyl-2-ethoxy-1,2-dihydro-quinoline (EEDQ), the saturation curves from both control and EEDQ-treated rats were
again best fitted to a two-site model. For EEDQ-treated animals, a drastic decrease of 5-HT1A receptor activity was noted; this loss was greater in hippocampus than in cerebral cortex. Since the decrease in 5-HT1A receptors was not associated with changes in low-affinity binding, the results suggest independent regulations of the two
[3H]8-OH-DPAT binding proteins. Altogether, the present data further supports the notion that [3H]8-OH-DPAT, besides labelling 5-HT1A receptors, also binds to other structures in rat cerebral cortex and hippocampus.
Special issue dedicated to Dr. Kinya Kuriyama 相似文献
32.
Soheila Ajdary Mohammad Hossein Hosseini Mohammad Hossein Alimohammadian 《Microbiology and immunology》1997,41(3):281-283
Interleukin-1 exacerbates leishmanial lesions in Leishmania major-infected BALB/c mice. Indomethacin can modulate the effect of IL-1, so at least part of the IL-1 effect on disease progression is due to the induction of prostaglandin synthesis. 相似文献
33.
James A. Gregory Ariel Shepley‐McTaggart Michelle Umpierrez Barry K. Hurlburt Soheila J. Maleki Hugh A. Sampson Stephen P. Mayfield M. Cecilia Berin 《Plant biotechnology journal》2016,14(7):1541-1550
Peanut allergy is an IgE‐mediated adverse reaction to a subset of proteins found in peanuts. Immunotherapy aims to desensitize allergic patients through repeated and escalating exposures for several months to years using extracts or flours. The complex mix of proteins and variability between preparations complicates immunotherapy studies. Moreover, peanut immunotherapy is associated with frequent negative side effects and patients are often at risk of allergic reactions once immunotherapy is discontinued. Allergen‐specific approaches using recombinant proteins are an attractive alternative because they allow more precise dosing and the opportunity to engineer proteins with improved safety profiles. We tested whether Ara h 1 and Ara h 2, two major peanut allergens, could be produced using chloroplast of the unicellular eukaryotic alga, Chlamydomonas reinhardtii. C. reinhardtii is novel host for producing allergens that is genetically tractable, inexpensive and easy to grow, and is able to produce more complex proteins than bacterial hosts. Compared to the native proteins, algal‐produced Ara h 1 core domain and Ara h 2 have a reduced affinity for IgE from peanut‐allergic patients. We further found that immunotherapy using algal‐produced Ara h 1 core domain confers protection from peanut‐induced anaphylaxis in a murine model of peanut allergy. 相似文献
34.
Fatemeh Tahmasebi Parichehr Pasbakhsh Keywan Mortezaee Soheila Madadi Shirin Barati Iraj Ragerdi Kashani 《Journal of cellular biochemistry》2019,120(6):10576-10586
Multiple sclerosis (MS) is a chronic inflammatory disease affecting the central nervous system (CNS). Despite introducing multiple immunomodulatory approaches for MS, there are still major concerns about possible ways for improving remyelination in this disease. Microglia exert essential roles in regulation of myelination processes, and interaction between colony-stimulating factor 1 (CSF1) with its receptor CSF1R is considered as a key regulator of microglial differentiation and survival. The aim of this study was to investigate possible roles for a CSF1R inhibitor PLX3397 in recovery of central myelination processes. Chronic demyelination was induced in mice by addition of 0.2% cuprizone to the chow for 12 weeks. Next, animals were undergoing a diet containing 290 mg/kg PLX3397 to induce microglial ablation. The PLX3397 treatment caused a significant decrease in the rate of expression for the CSF1/CSF1R axis, and a reduction in the protein expressions for the microglial marker Iba-1 and for the oligodendrocyte marker Olig-2. Findings from Luxol fast blue (LFB) staining and transmission electron microscopy (TEM) showed an increase in the rate of myelination for the mice receiving PLX3397. The rate of destruction in the nerve fibers and the extent of the gaps formed between layers of myelin sheaths was also reduced after the treatment with PLX3397. In addition, animals experienced an improvement in recovery of motor deficit after receiving PLX3397 (for all P < 0.05). It could be concluded that PLX3397 could retain myelination in the MS model possibly through regulation of the myelin environment. 相似文献
35.
Parnian Javad Hoseindokht Maryam Khademi Zahra Moosavi Maedeh Soheili Zahra Soheila Samie Shahram Zomorodipour Alireza 《Biotechnology letters》2022,44(5):713-728
Biotechnology Letters - To improve the expression efficiency of recombinant hFIX, by enhancing its γ-carboxylation, which is inhibited by Calumenin (CALU), we used intronic artificial... 相似文献
36.
Mozhgan Khorasani-Motlagh Meissam Noroozifar Soheila Khajeh 《Inorganica chimica acta》2009,362(10):3785-1294
The molecular structure of praseodymium (III) complex with 1,10-phenanthroline (phen), [Pr(phen)2Cl3·OH2] (1) was determined by single-crystal X-ray diffraction. Crystal data: crystal system, triclinic, space group P and Z = 2, a = 7.1110(7) ?, b = 10.1716(10) ?, c = 17.2367(18) ?, α = 80.922(5)°, β = 78.759(5)°, γ = 70.151(5)°, R1 = 0.036; wR2 = 0.076 for all data. Treatment of aqueous solution of [Pr(phen)2Cl3·OH2] (1) with thallium phenylcyanamide salts yield [Pr(phen)2(L)3] (L = pcyd (2), 2-Clpcyd (3), 2,3,5-Cl3pcyd (4), 2,3,4,5-Cl4pcyd (5)). Four new praseodymium (III) complexes have been characterized by IR, UV-Vis and 1H NMR spectroscopy as well as elemental analysis. The 1H NMR spectra of these complexes show broadening of ligand protons attributed to coordination of paramagnetic center. 相似文献
37.
Chrysoula Vasileiou Kin Sing Stephen Lee Rachael M. Crist Soheila Vaezeslami Sarah M. Goins James H. Geiger Babak Borhan 《Proteins》2009,76(2):281-290
The binding of retinoic acid to mutants of Cellular Retinoic Acid Binding Protein II (CRABPII) was evaluated to better understand the importance of the direct protein/ligand interactions. The important role of Arg111 for the correct structure and function of the protein was verified and other residues that directly affect retinoic acid binding have been identified. Furthermore, retinoic acid binding to CRABPII mutants that lack all previously identified interacting amino acids was rescued by providing a carboxylic acid dimer partner in the form of a Glu residue. Proteins 2009. © 2008 Wiley‐Liss, Inc. 相似文献
38.
Sepideh N. Bajestan Fujio Umehara Yuko Shirahama Kayoko Itoh Soheila Sharghi‐Namini Kristjan R. Jessen Rhona Mirsky Mitsuhiro Osame 《Developmental neurobiology》2006,66(3):243-255
Hedgehog proteins are important in the development of the nervous system. As Desert hedgehog (Dhh) is involved in the development of peripheral nerves and is expressed in adult nerves, it may play a role in the maintenance of adult nerves and degeneration and regeneration after injury. We firstly investigated the Dhh‐receptors, which are expressed in mouse adult nerves. The Dhh receptor patched(ptc)2 was detected in adult sciatic nerves using RT‐PCR, however, ptc1 was undetectable under the same experimental condition. Using RT‐PCR in purified cultures of mouse Schwann cells and fibroblasts, we found ptc2 mRNA in Schwann cells, and at much lower levels, in fibroblasts. By immunohistochemistry, Ptc2 protein was seen on unmyelinated nerve fibers. Then we induced crush injury to the sciatic nerves of wild‐type (WT) and dhh‐null mice and the distal stumps of injured nerves were analyzed morphologically at different time points and expression of dhh and related receptors was also measured by RT‐PCR in WT mice. In dhh‐null mice, degeneration of myelinated fibers was more severe than in WT mice. Furthermore, in regenerated nerves of dhh‐null mice, minifascicular formation was even more extensive than in dhh‐null intact nerves. Both dhh and ptc2 mRNA levels were down‐regulated during the degenerative phase postinjury in WT mice, while levels rose again during the phase of nerve regeneration. These results suggest that the Dhh‐Ptc2 signaling pathway may be involved in the maintenance of adult nerves and may be one of the factors that directly or indirectly determines the response of peripheral nerves to injury. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2006 相似文献
39.
Snail regulates cell survival and inhibits cellular senescence in human metastatic prostate cancer cell lines 总被引:1,自引:0,他引:1
Modjtaba Emadi Baygi Zahra Soheila Soheili Ingo Schmitz Shahram Sameie Wolfgang A. Schulz 《Cell biology and toxicology》2010,26(6):553-567
The epithelial–mesenchymal transition (EMT) is regarded as an important step in cancer metastasis. Snail, a master regulator
of EMT, has been recently proposed to act additionally as a cell survival factor and inducer of motility. We have investigated
the function of Snail (SNAI1) in prostate cancer cells by downregulating its expression via short (21-mer) interfering RNA (siRNA) and measuring the consequences
on EMT markers, cell viability, death, cell cycle, senescence, attachment, and invasivity. Of eight carcinoma cell lines,
the prostate carcinoma cell lines LNCaP and PC-3 showed the highest and moderate expression of SNAI1 mRNA, respectively, as measured by quantitative RT-PCR. Long-term knockdown of Snail induced a severe decline in cell numbers
in LNCaP and PC-3 and caspase activity was accordingly enhanced in both cell lines. In addition, suppression of Snail expression
induced senescence in LNCaP cells. SNAI1-siRNA-treated cells did not tolerate detachment from the extracellular matrix, probably
due to downregulation of integrin α6. Expression of E-cadherin, vimentin, and fibronectin was also affected. Invasiveness
of PC-3 cells was not significantly diminished by Snail knockdown. Our data suggest that Snail acts primarily as a survival
factor and inhibitor of cellular senescence in prostate cancer cell lines. We therefore propose that Snail can act as early
driver of prostate cancer progression. 相似文献
40.
Hiroshi Shinmoto Yasunori Naganawa Michie Shimmoto Soheila J. Maleki 《Cytotechnology》2004,46(1):19-23
Two clones of mouse–human hybridomas, secreting human monoclonal antibodies to a peanut allergen Ara h1, were generated from human peripheral blood lymphocytes transformed with Epstein--Barr virus, followed by cell fusion with mouse myeloma cells. Epitope analysis with overlapping peptides synthesized on a multi-pin apparatus revealed antibody-binding sequences of Ara h1 protein. 相似文献