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Great Bustards are still fairly abundant (5-2 nests/1000 ha) in Zamora. Overall fecundity was high (92% of adult females attempt to nest; 2–47 eggs/clutch; 89% egg fertility), but pre- and post-hatching mortality were also high (50% of eggs laid; 57% of nestlings hatched), due mainly to agricultural machinery but also to natural predators. Calculated annual yields per 100 adult females were: 227 eggs, 102 hatchlings, 44 fledglings. Production of 44 fledglings/y could sustain a stable adult population (mortality approx. 8%/y) if mortality of immatures is not more than 18–22%/y. 相似文献
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The astragalus of Paleogene artiodactyls: comparative morphology, variability and prediction of body mass 总被引:1,自引:0,他引:1
JEAN-NOËL MARTINEZ JEAN SUDRE 《Lethaia: An International Journal of Palaeontology and Stratigraphy》1995,28(3):197-209
Martinez, J.-N. & Sudre, 1. 1995 11 30 The astragalus of Paleogene artiodactyls: comparative morphology, variability and prediction of body mass. Lethaia , Vol. 28, pp. 197–209. Oslo. ISSN 0024–1164.
The morphology of the astragalus is analysed in nineteen Paleogene artiodactyls (suborders Palaeodonta, Suina, Ancodonta and Ruminantia). This morphology is related to the functional adaptations of the appendicular skeleton, but some diagnostic characters can be seen at the family level. For the populations examined, the proportions of this bone show a low intraspecific variability, which does not allow detection of any dimorphism. An allometric relationship between the dimensions of the astragalus and the body mass has been established for extant species, allowing estimates for the fossil species. In most cases, the interval between the two extreme estimations using the astragalus includes the estimated body mass using M/1 area. The limits of this method are discussed, and it is suggested that the dimensions of the astragalus give a better estimation of the body mass than the dental area. □ Artiodactyls, astragalus, comparative morphology, body mass, allometry, Paleogene . 相似文献
The morphology of the astragalus is analysed in nineteen Paleogene artiodactyls (suborders Palaeodonta, Suina, Ancodonta and Ruminantia). This morphology is related to the functional adaptations of the appendicular skeleton, but some diagnostic characters can be seen at the family level. For the populations examined, the proportions of this bone show a low intraspecific variability, which does not allow detection of any dimorphism. An allometric relationship between the dimensions of the astragalus and the body mass has been established for extant species, allowing estimates for the fossil species. In most cases, the interval between the two extreme estimations using the astragalus includes the estimated body mass using M/1 area. The limits of this method are discussed, and it is suggested that the dimensions of the astragalus give a better estimation of the body mass than the dental area. □ Artiodactyls, astragalus, comparative morphology, body mass, allometry, Paleogene . 相似文献
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MARÍA DOLORES LÓPEZ-LUCAS GISELA PACHÓN-PEÑA ANA MARÍA GARCÍA-HERNÁNDEZ ANTONIO PARRADO DARÍO SÁNCHEZ-SALINAS DAVID GARCÍA-BERNAL MARIA DEL CARMEN ALGUERÓ FRANCISCA INIESTA MARTINEZ MIGUEL BLANQUER VALENTÍN CABAÑAS-PERIANES MAR MOLINA-MOLINA CIRA ASÍN-AGUILAR JOSÉ M MORALEDA ROBERT SACKSTEIN 《Cytotherapy》2018,20(9):1110-1123
Background
The regenerative and immunomodulatory properties of human mesenchymal stromal cells (hMSCs) have raised great hope for their use in cell therapy. However, when intravenously infused, hMSCs fail to reach sites of tissue injury. Fucose addition in α(1,3)-linkage to terminal sialyllactosamines on CD44 creates the molecule known as hematopoietic cell E-/L-selectin ligand (HCELL), programming hMSC binding to E-selectin that is expressed on microvascular endothelial cells of bone marrow (BM), skin and at all sites of inflammation. Here we describe how this modification on BM-derived hMSCs (BM-hMSCs) can be adapted to good manufacturing practice (GMP) standards.Methods
BM-hMSCs were expanded using xenogenic-free media and exofucosylated using α(1,3)-fucosyltransferases VI (FTVI) or VII (FTVII). Enforced fucosylation converted CD44 into HCELL, and HCELL formation was assessed using Western blot, flow cytometry and cell-binding assays. Untreated (unfucosylated), buffer-treated and exofucosylated BM-hMSCs were each analyzed for cell viability, immunophenotype and differentiation potential, and E-selectin binding stability was assessed at room temperature, at 4°C, and after cryopreservation. Cell product safety was evaluated using microbiological testing, karyotype analysis, and c-Myc messenger RNA (mRNA) expression, and potential effects on genetic reprogramming and in cell signaling were analyzed using gene expression microarrays and receptor tyrosine kinase (RTK) phosphorylation arrays.Results
Our protocol efficiently generates HCELL on clinical-scale batches of BM-hMSCs. Exofucosylation yields stable HCELL expression for 48 h at 4°C, with retained expression after cell cryopreservation. Cell viability and identity are unaffected by exofucosylation, without changes in gene expression or RTK phosphorylation.Discussion
The described exofucosylation protocol using xenogenic-free reagents enforces HCELL expression on hMSCs endowing potent E-selectin binding without affecting cell viability or native phenotype. This described protocol is readily scalable for GMP-compliant clinical production. 相似文献95.
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