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851.
W I Gudim T G Sukiasowa W S Iwanowa N A Gorbunowa G I Kosinez 《Folia haematologica (Leipzig, Germany : 1928)》1987,114(6):834-844
Fraction F1 with a MW of 5.10(3)-5.10(4), which had been isolated from the blood serum of blood donors as controls and patients at the terminal stage of chronic kidney insufficiency, specifically inhibited the erythropoiesis of test animals at the stage sensitive to erythropoietin and morphologically distinguishable cells of the bone-marrow. In uremia, protein concentration was twice higher in comparison to the normal one. The inhibiting effect of fraction F1 from the serum of patients is caused by the enlargement of level of physiological inhibitors and unspecific toxins of the cell proliferation. Those data characterising the factor detected in the fraction F1 of normal serum together with the erythrocytic chalon are discussed. 相似文献
852.
Some properties of hexameric purine nucleoside phosphorylase II (EC 2.4.2.1) from Escherichia coli K-12 were studied. The enzyme obeys the Michaelis-Menten kinetics with respect to purine substrates (Km for inosine, deoxyinosine and hypoxanthine are equal to 492, 106 and 26.6 microM, respectively) and exhibits negative kinetic cooperativity towards phosphate and ribose-1-phosphate. The Hill coefficient is equal to approximately 0.5 for both substrates. Hexameric purine nucleoside phosphorylase II is not a metal-dependent enzyme; its activity is inhibited by Cu2+, Zn2+, Ni2+ and SO4(2-). The enzyme is the most stable at pH 6.0; it contains essential thiol groups. All substrates partly protect the enzyme against inactivation by 5.5'-dithiobis(2-nitrobenzoic acid) and heat-inactivation and, with the exception of phosphate-against inactivation by p-chloromercuribenzoate. Hypoxanthine, especially in combination with phosphate, afford the best protection against inactivation. 相似文献
853.
854.
The chromosomes of two patients with ring 13 (r13) were studied using high-resolution RBG banding of prometaphase cells. The rings of the two patients differ slightly in breakpoints. Cell with multiple single, double-sized rings, quadruple-sized rings, rod- and ring-shaped fragments, and fragments showing varied states of condensation were seen, as were cells monosomic for chromosome 13. The evolution of these cell lines as a result of sister chromatid exchange, nondisjunction, ring breakage, and premature chromosome condensation is discussed. Clinical features of these patients reflect the heterogeneity of phenotype for r13 patients. Each case includes a feature of trisomy 13. The significance of mosaicism of cell lines in patients bearing ring chromosomes is considered with respect to variation in clinical findings. 相似文献
855.
K A Abo-Hashema M H Cake I C Potter 《Biochemical and biophysical research communications》1999,258(3):778-783
Succinate dehydrogenase activity in mitochondria, which were isolated by centrifuging partially purified mitochondria through 1. 315 M sucrose, was completely suppressed when [14C]succinate uptake was abolished by prior incubation of the mitochondria with carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) and valinomycin. The conclusion that these mitochondria were intact was confirmed by the fact that, when these mitochondria were broken by a freeze-thaw cycle followed by sonication, such inhibition was totally abolished. The yield of mitochondria, microsomes, and peroxisomes from the initial homogenate was 17.8, <0.1, and 0%, respectively, indicating that the mitochondria were not only intact but also essentially free of contamination from microsomes and peroxisomes. The overt form of carnitine palmitoyltransferase (CPT I) in these intact and pure mitochondria was totally inhibited by malonyl CoA, indicating that previous reports of incomplete inhibition in mitochondrial preparations resulted from interference from CPT activity in the inner mitochondrial membrane (CPT II), microsomes, or peroxisomes. 相似文献
856.
Evidence is presented that lactoferrin (LF), an Fe3+-binding glycoprotein, possesses two DNA-binding sites with different affinities for specific oligonucleotides (ODNs) (Kdl = 8 nM; Kd2 approximately 0.1 mM). The high affinity site became labeled after incubation with affinity probes for DNA-binding sites; like the antibacterial and polyanion-binding sites, this site was shown to be located in the N-terminal domain of LF. Interaction of heparin with the polyanion-binding site inhibits the binding of ODNs to both sites. These data suggest that the DNA-binding sites of LF coincide or overlap with the known polyanion and antimicrobial domains of the protein. 相似文献
857.
V Krajca 《Activitas nervosa superior》1984,26(2):118-128
Use of the dynamic clusters method for automatic extraction of compressed information about recorded EEG signal is presented. The computer first divides the record into quasi-stationary segments by means of adaptive segmentation. Second, the extracted segments are classified by a method of dynamic clusters into homogeneous classes. One part of the used clustering algorithm permits to specify and draw the most typical class members, which may represent the whole studied EEG signal and may be used as input for the further phase of the automatic EEG analysis, i.e. for the classification of the whole EEG records. The above procedure was applied to a 75 sec long EEG record of anaesthetized cat intoxicated by CO. 相似文献
858.
S K Tandon A K Mathur J R Behari S V Chandra 《Acta biologica et medica Germanica》1975,34(11-12):1835-1842
Daily intraperitoneal administration of thorium nitrate produced progressive morphological and biochemical alterations with the increase in thorium concentration in rat testis. The degenerative changes in the seminiferous tubules increased with the duration of treatment and after 90 days calcification occurred in about 25% of the tubules and in the connective tissue of the tunica albuginea. The activity of adenosine triphosphatase and alkaline phosphatase increased markedly as a result of thorium administration. An attempt has been made to interrelate histopathological and enzymatic changes and the metal concentration in the testicular tissue. 相似文献
859.
860.