全文获取类型
收费全文 | 321988篇 |
免费 | 21686篇 |
国内免费 | 61篇 |
出版年
2021年 | 2689篇 |
2020年 | 2211篇 |
2019年 | 2516篇 |
2018年 | 5457篇 |
2017年 | 5159篇 |
2016年 | 6655篇 |
2015年 | 7634篇 |
2014年 | 8923篇 |
2013年 | 12399篇 |
2012年 | 14898篇 |
2011年 | 14963篇 |
2010年 | 10110篇 |
2009年 | 8126篇 |
2008年 | 12858篇 |
2007年 | 13120篇 |
2006年 | 12347篇 |
2005年 | 11512篇 |
2004年 | 11332篇 |
2003年 | 10731篇 |
2002年 | 10342篇 |
2001年 | 11207篇 |
2000年 | 10821篇 |
1999年 | 8602篇 |
1998年 | 2826篇 |
1997年 | 2509篇 |
1996年 | 2314篇 |
1995年 | 2155篇 |
1992年 | 6596篇 |
1991年 | 6727篇 |
1990年 | 6582篇 |
1989年 | 6467篇 |
1988年 | 6022篇 |
1987年 | 5561篇 |
1986年 | 5091篇 |
1985年 | 5379篇 |
1984年 | 4316篇 |
1983年 | 3537篇 |
1982年 | 2416篇 |
1979年 | 3883篇 |
1978年 | 2992篇 |
1977年 | 2751篇 |
1976年 | 2693篇 |
1975年 | 3220篇 |
1974年 | 3533篇 |
1973年 | 3509篇 |
1972年 | 3057篇 |
1971年 | 2915篇 |
1970年 | 2494篇 |
1969年 | 2424篇 |
1968年 | 2279篇 |
排序方式: 共有10000条查询结果,搜索用时 31 毫秒
781.
Homologies between paraflagellar rod proteins from trypanosomes and euglenoids revealed by a monoclonal antibody 总被引:2,自引:0,他引:2
A Nonidet P 40 insoluble fraction was isolated from Trypanosoma brucei and was used to raise a monoclonal antibody (5E9). The antigen was localized by indirect immunofluorescence in the flagellum of T. brucei and of two species of euglenoids, Euglena gracilis and Distigma proteus. In immunoblot analysis, 5E9 appeared to bind to paraflagellar rod proteins PFR1 and PFR2 of T. brucei (72000 and 75000 mol. wt.) and of E. gracilis (67000 and 76000 mol. wt.). The presence of a common epitope in paraflagellar rod proteins from species of trypanosomes and euglenoids shows that despite distinct structures of the rods some identical domain exists in the proteins that could be involved in their supramolecular assembly into a similar organelle. The antigenic determinant defined by 5E9 was also shown to be present in a 87000 molecular weight polypeptide located in the proximal part of the flagellum of Crithidia oncopelti in which a paraflagellar rod is not detectable at the ultrastructural level. 相似文献
782.
The relationship of hormone-sensitive and hormone-insensitive phosphatidylinositol to phosphatidylinositol 4,5-bisphosphate in the WRK-1 cell 总被引:14,自引:0,他引:14
We have previously characterized two distinct pools of phosphatidylinositol (PI) in the WRK-1 rat mammary tumor cell, one whose metabolism is enhanced in response to vasopressin and another which is insensitive to hormonal manipulation. The purpose of the present study was to examine the relationship between cellular phosphatidylinositol 4,5-bisphosphate (PIP2) and each of the two PI pools. We have found that in WRK-1 cells, vasopressin induces the rapid loss of PIP2 and the accumulation of inositol phosphates. By making use of kinetic differences in 32Pi uptake into the two pools of PI and assessing radioactivity levels in the 1-phosphate of PIP2, we have determined that hormone-sensitive PI is the precursor of approximately 60% of the cellular PIP2; the remainder is synthesized from the hormone-insensitive pool. Additional data indicate that PIP2 derived from hormone-sensitive PI is likewise hormone-sensitive, while that synthesized from hormone-insensitive PI remains stable over a long period of time and is not affected by the presence of vasopressin. 相似文献
783.
Different secretory pathways of renin from mouse cells transfected with the human renin gene 总被引:5,自引:0,他引:5
R E Pratt J A Flynn P M Hobart M Paul V J Dzau 《The Journal of biological chemistry》1988,263(7):3137-3141
Mammalian cells in culture, transfected with human renin gene, can provide a useful tool for studying renin biosynthesis and secretion. We transfected fibroblast cells (mouse L929 and Chinese hamster ovary cells) and pituitary tumor cells (mouse AtT-20) with the human renin gene and a selectable plasmid (pSV2Neo). Transfected fibroblasts synthesize prorenin only. Prorenin is secreted by fibroblasts constitutively and the secretion is not influenced by 8-bromo-cAMP. On the other hand, transfected AtT-20 cells synthesized both prorenin and mature active renin. Transfected AtT-20 cells release prorenin by constitutive secretion but mature renin is secreted by a regulated mechanism since the secretion of the former is not influenced by 8-bromo-cAMP but the release of the latter is significantly stimulated. Our studies demonstrate that human renin may be secreted by at least two cellular pathways: prorenin by a constitutive pathway and mature renin by a regulated pathway. These transfected cells may provide useful models for studies of human renin synthesis, processing, and secretion. 相似文献
784.
785.
786.
Characterization of the specific binding of rat apolipoprotein E-deficient HDL to rat hepatic plasma membranes 总被引:1,自引:0,他引:1
We have used a preparation of rat liver plasma membranes to study the binding of rat apolipoprotein E-deficient HDL to rat liver. The membranes were found to bind HDL by a saturable process that was competed for by excess unlabeled HDL. The binding was temperature-dependent and was 85% receptor-mediated when incubated at 4, 22 and 37 degrees C. The affinity of the binding site for the HDL was consistent at all temperatures, while the maximum binding capacity increased at higher temperatures. The specific binding of HDL to the membranes did not require calcium and was independent of the concentration of NaCl in the media. The effect of varying the pH of the media on HDL binding was small, being 30% higher at pH 6.5 than at pH 9.0. Both rat HDL and human HDL3 were found to compete for the binding of rat HDL to the membranes, whereas rat VLDL remnants and human LDL did not compete. At 4 degrees C, complexes of dimyristoylphosphatidylcholine (DMPC) and apolipoproteins A-I, A-IV and the C apolipoproteins, but not apolipoprotein E, competed for HDL binding to the membranes. At 22 and 37 degrees C, all DMPC-apolipoprotein complexes competed to a similar extent, DMPC vesicles that contained no protein did not compete for the binding of HDL. These results suggest that the rat liver possesses a specific receptor for apolipoprotein E-deficient HDL that recognizes apolipoproteins A-I, A-IV and the C apolipoproteins as ligands. 相似文献
787.
788.
789.
790.