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排序方式: 共有315条查询结果,搜索用时 62 毫秒
61.
Stephen R. Williams Qiong Yang Fang Chen Xuan Liu Keith L. Keene Paul Jacques Wei-Min Chen Galit Weinstein Fang-Chi Hsu Alexa Beiser Liewei Wang Ebony Bookman Kimberly F. Doheny Philip A. Wolf Michelle Zilka Jacob Selhub Sarah Nelson Stephanie M. Gogarten Bradford B. Worrall Sudha Seshadri Michèle M. Sale the Genomics Randomized Trials Network the Framingham Heart Study 《PLoS genetics》2014,10(3)
62.
Meshulam-Simon G Behrens S Choo AD Spormann AM 《Applied and environmental microbiology》2007,73(4):1153-1165
Shewanella oneidensis MR-1 is a facultative sediment microorganism which uses diverse compounds, such as oxygen and fumarate, as well as insoluble Fe(III) and Mn(IV) as electron acceptors. The electron donor spectrum is more limited and includes metabolic end products of primary fermenting bacteria, such as lactate, formate, and hydrogen. While the utilization of hydrogen as an electron donor has been described previously, we report here the formation of hydrogen from pyruvate under anaerobic, stationary-phase conditions in the absence of an external electron acceptor. Genes for the two S. oneidensis MR-1 hydrogenases, hydA, encoding a periplasmic [Fe-Fe] hydrogenase, and hyaB, encoding a periplasmic [Ni-Fe] hydrogenase, were found to be expressed only under anaerobic conditions during early exponential growth and into stationary-phase growth. Analyses of DeltahydA, DeltahyaB, and DeltahydA DeltahyaB in-frame-deletion mutants indicated that HydA functions primarily as a hydrogen-forming hydrogenase while HyaB has a bifunctional role and represents the dominant hydrogenase activity under the experimental conditions tested. Based on results from physiological and genetic experiments, we propose that hydrogen is formed from pyruvate by multiple parallel pathways, one pathway involving formate as an intermediate, pyruvate-formate lyase, and formate-hydrogen lyase, comprised of HydA hydrogenase and formate dehydrogenase, and a formate-independent pathway involving pyruvate dehydrogenase. A reverse electron transport chain is potentially involved in a formate-hydrogen lyase-independent pathway. While pyruvate does not support a fermentative mode of growth in this microorganism, pyruvate, in the absence of an electron acceptor, increased cell viability in anaerobic, stationary-phase cultures, suggesting a role in the survival of S. oneidensis MR-1 under stationary-phase conditions. 相似文献
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66.
Enrichment of high affinity subclasses and glycoforms from serum‐derived IgG using FcγRs as affinity ligands
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67.
Shir Zer-Krispil Hila Zak Lisha Shao Shir Ben-Shaanan Lea Tordjman Assa Bentzur Anat Shmueli Galit Shohat-Ophir 《Current biology : CB》2018,28(9):1445-1452.e3
68.
Localization of an Ataxia-Telangiectasia Gene to an −500-kb Interval on Chromosome 11q23.1: Linkage Analysis of 176 Families by an International Consortium
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Ethan Lange Anna-Lise Borresen Xiaoguang Chen Luciana Chessa Sujata Chiplunkar Patrick Concannon Sugandha Dandekar Steven Gerken Kenneth Lange Teresa Liang Carmel McConville Jeff Polakow Oscar Porras Galit Rotman Ozden Sanal Sepideh Sheikhavandi Yosef Shiloh Eric Sobel Malcolm Taylor Milhan Telatar Sharon Teraoka Aslihan Tolun Nitin Udar Nancy Uhrhammer Lina Vanagaite Zhijun Wang Beth Wapelhorst Jocyndra Wright Huan-Ming Yang Lan Yang Yael Ziv Richard A. Gatti 《American journal of human genetics》1995,57(1):112-119
We describe a 20-point linkage analysis map of chromosome 11q22-23 that is based on genotyping 249 families (59 CEPH and 190 A-T). Monte Carlo linkage analyses of 176 ataxia-telangiectasia (A-T) families localizes the major A-T locus to the region between S1819(A4) and S1818(A2). When seven nonlinking families were excluded from subsequent analyses, a 2-lod support interval of ~500 kb was identified between S1819(A4) and S1294. No recombinants were observed between A-T and markers S384, B7, S535, or S1294. Only 17 of the international consortium families have been assigned to complementation groups. The available evidence favors either a cluster of A-T genes on chromosome 11 or intragenic defects in a single gene. 相似文献
69.
Sara Orgad Galit Rosenfeld Sarit Smolikove Talia Polak Daniel Segal 《Invertebrate neuroscience : IN》1997,3(2-3):175-183
We describe six recessive autosomal male sterile mutations inDrosophila, generated by mobilization of single P-elements, exhibiting abnormal male courtship behavior. Detailed analysis of courtship
behavior elicited by virgin wild type females indicated that five of the six mutants are affected in the early steps of courtship.
The sixth mutant is blocked at the step of attempted copulation which occurs later in the courtship sequence. All of the mutants
have normal olfactory responses and normal locomotor activity. No defect in the visual modality has been observed for the
five mutants affected in the initiation of courtship. The mutant blocked at attempted copulation lacks the ‘on’ and ‘off’
transients, but this appears to be due to genetic background rather than the mutation itself. Abnormal spermatogenesis was
observed in five of the mutants. Spermatogenic defects vary and include lesions in the proliferation of the germline, in meiosis,
and in the differentiation and maturation of the spermatids into motile sperm. 相似文献
70.
Alison E. Mahan Madeleine F. Jennewein Todd Suscovich Kendall Dionne Jacquelynne Tedesco Amy W. Chung Hendrik Streeck Maria Pau Hanneke Schuitemaker Don Francis Patricia Fast Dagna Laufer Bruce D. Walker Lindsey Baden Dan H. Barouch Galit Alter 《PLoS pathogens》2016,12(3)
Antibody effector functions, such as antibody-dependent cellular cytotoxicity, complement deposition, and antibody-dependent phagocytosis, play a critical role in immunity against multiple pathogens, particularly in the absence of neutralizing activity. Two modifications to the IgG constant domain (Fc domain) regulate antibody functionality: changes in antibody subclass and changes in a single N-linked glycan located in the CH2 domain of the IgG Fc. Together, these modifications provide a specific set of instructions to the innate immune system to direct the elimination of antibody-bound antigens. While it is clear that subclass selection is actively regulated during the course of natural infection, it is unclear whether antibody glycosylation can be tuned, in a signal-specific or pathogen-specific manner. Here, we show that antibody glycosylation is determined in an antigen- and pathogen-specific manner during HIV infection. Moreover, while dramatic differences exist in bulk IgG glycosylation among individuals in distinct geographical locations, immunization is able to overcome these differences and elicit antigen-specific antibodies with similar antibody glycosylation patterns. Additionally, distinct vaccine regimens induced different antigen-specific IgG glycosylation profiles, suggesting that antibody glycosylation is not only programmable but can be manipulated via the delivery of distinct inflammatory signals during B cell priming. These data strongly suggest that the immune system naturally drives antibody glycosylation in an antigen-specific manner and highlights a promising means by which next-generation therapeutics and vaccines can harness the antiviral activity of the innate immune system via directed alterations in antibody glycosylation in vivo. 相似文献