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991.
Qinghua Wu Kamil Kuča Hans-Ulrich Humpf Blanka Klímová Benedikt Cramer 《Mycotoxin Research》2017,33(1):79-91
Deoxynivalenol (DON), the most commonly occurring trichothecene in nature, may affect animal and human health through causing diarrhea, vomiting, gastrointestinal inflammation, and immunomodulation. DON-3-glucoside (DON-3G) as a major plant metabolite of the mycotoxin is another “emerging” food safety issue in recent years. Humans may experience potential health risks by consuming DON-contaminated food products. Thus, it is crucial for human and animal health to study also the degradation of DON and DON-3G during thermal food processing. Baking, boiling, steaming, frying, and extrusion cooking are commonly used during thermal food processing and have promising effects on the reduction of mycotoxins in food. For DON, however, the observed effects of these methods, as reported in numerous studies, are ambiguous and do not present a clear picture with regard to reduction or transformation. This review summarized the influence of thermal processing on the stability of DON and the formation of degradation/conversion products. Besides this, also a release of DON and DON-3G from food matrix as well as the release of DON from DON-3G during processing is discussed. In addition, some conflicting findings as reported from the studies on thermal processing as well as cause-effect relationships of the different thermal procedures are explored. Finally, the potential toxic profiles of DON degradation products are discussed as well when data are available. 相似文献
992.
Sebastian T. Opitz Andreas Blankenstein Ute Harms 《Journal of biological education》2017,51(4):427-440
The concept of energy serves biologists as a powerful analytical model to describe phenomena that occurs in the natural world. Due to the concept’s relevance, educational standards of different countries identify energy as a core idea for the teaching and learning of biology and other science subjects. However, previous research on students’ energy understanding has mostly focused on physics contexts. This cross-sectional study extends insight to the field by providing a systematic analysis of students’ (N = 30, grades 5, 7, 9, 11) conceptions about energy in biological contexts. In order to connect the findings to previous research, the study analyses conceptions about four energy aspects that are regarded as central for understanding the concept in different disciplinary contexts, i.e. (1) energy forms/sources, (2) transfer/transformation, (3) degradation/dissipation and (4) energy conservation. The findings identify substantial changes in students’ conceptions about energy between the different grade levels, but also highlight conceptions that students consistently employed across age groups. The results are discussed in the light of previous research on students’ progressing energy understanding and the connection of their energy understanding across different disciplinary contexts. Lastly, the article provides implications for the further development of energy teaching in biological contexts. 相似文献
993.
Klécia G. Massi Michael Bird Beatriz S. Marimon Ben Hur MarimonJr. Denis S. Nogueira Edmar A. Oliveira Oliver L. Phillips Carlos A. Quesada Ana S. Andrade Roel J. W. Brienen José L. C. Camargo Jerome Chave Eurídice N. Honorio Coronado Leandro V. Ferreira Niro Higuchi Susan G. Laurance William F. Laurance Thomas Lovejoy Yadvinder Malhi Rodolfo V. Martínez Abel Monteagudo David Neill Adriana Prieto Hirma Ramírez-Angulo Hans ter Steege Emilio Vilanova Ted R. Feldpausch 《Plant Ecology》2017,218(9):1047-1062
Amazon forests are fire-sensitive ecosystems and consequently fires affect forest structure and composition. For instance, the legacy of past fire regimes may persist through some species and traits that are found due to past fires. In this study, we tested for relationships between functional traits that are classically presented as the main components of plant ecological strategies and environmental filters related to climate and historical fires among permanent mature forest plots across the range of local and regional environmental gradients that occur in Amazonia. We used percentage surface soil pyrogenic carbon (PyC), a recalcitrant form of carbon that can persist for millennia in soils, as a novel indicator of historical fire in old-growth forests. Five out of the nine functional traits evaluated across all 378 species were correlated with some environmental variables. Although there is more PyC in Amazonian soils than previously reported, the percentage soil PyC indicated no detectable legacy effect of past fires on contemporary functional composition. More species with dry diaspores were found in drier and hotter environments. We also found higher wood density in trees from higher temperature sites. If Amazon forest past burnings were local and without distinguishable attributes of a widespread fire regime, then impacts on biodiversity would have been small and heterogeneous. Alternatively, sufficient time may have passed since the last fire to allow for species replacement. Regardless, as we failed to detect any impact of past fire on present forest functional composition, if our plots are representative then it suggests that mature Amazon forests lack a compositional legacy of past fire. 相似文献
994.
Katie?E.?Hillyer Daniel?A.?Dias Adrian?Lutz Shaun?P.?Wilkinson Ute?Roessner Simon?K.?DavyEmail author 《Coral reefs (Online)》2017,36(1):105-118
Rising seawater temperatures pose a significant threat to the persistence of coral reefs. Despite the importance of these systems, major gaps remain in our understanding of how thermal stress and bleaching affect the metabolic networks that underpin holobiont function. We applied gas chromatography–mass spectrometry (GC–MS) metabolomics to detect changes in the intracellular free metabolite pools (polar and semi-polar compounds) of in hospite dinoflagellate symbionts and their coral hosts (and any associated microorganisms) during early- and late-stage thermal bleaching (a reduction of approximately 50 and 70% in symbiont density, respectively). We detected characteristic changes to the metabolite profiles of each symbiotic partner associated with individual cellular responses to thermal, oxidative and osmotic stress, which progressed with the severity of bleaching. Alterations were also indicative of changes to energy-generating and biosynthesis pathways in both partners, with a shift to the increased catabolism of lipid stores. Specifically, in symbiont intracellular metabolite pools, we observed accumulations of multiple free fatty acids, plus the chloroplast-associated antioxidant alpha-tocopherol. In the host, we detected a decline in the abundance of pools of multiple carbohydrates, amino acids and intermediates, in addition to the antioxidant ascorbate. These findings further our understanding of the metabolic changes that occur to symbiont and host (and its associated microorganisms) during thermal bleaching. These findings also provide further insight into the largely undescribed roles of free metabolite pools in cellular homeostasis, signalling and acclimation to thermal stress in the cnidarian–dinoflagellate symbiosis. 相似文献
995.
Ulrich Brose Julia L. Blanchard Anna Eklöf Nuria Galiana Martin Hartvig Myriam R. Hirt Gregor Kalinkat Marie C. Nordström Eoin J. O'Gorman Björn C. Rall Florian D. Schneider Elisa Thébault Ute Jacob 《Biological reviews of the Cambridge Philosophical Society》2017,92(2):684-697
Understanding the consequences of species loss in complex ecological communities is one of the great challenges in current biodiversity research. For a long time, this topic has been addressed by traditional biodiversity experiments. Most of these approaches treat species as trait‐free, taxonomic units characterizing communities only by species number without accounting for species traits. However, extinctions do not occur at random as there is a clear correlation between extinction risk and species traits. In this review, we assume that large species will be most threatened by extinction and use novel allometric and size‐spectrum concepts that include body mass as a primary species trait at the levels of populations and individuals, respectively, to re‐assess three classic debates on the relationships between biodiversity and (i) food‐web structural complexity, (ii) community dynamic stability, and (iii) ecosystem functioning. Contrasting current expectations, size‐structured approaches suggest that the loss of large species, that typically exploit most resource species, may lead to future food webs that are less interwoven and more structured by chains of interactions and compartments. The disruption of natural body‐mass distributions maintaining food‐web stability may trigger avalanches of secondary extinctions and strong trophic cascades with expected knock‐on effects on the functionality of the ecosystems. Therefore, we argue that it is crucial to take into account body size as a species trait when analysing the consequences of biodiversity loss for natural ecosystems. Applying size‐structured approaches provides an integrative ecological concept that enables a better understanding of each species' unique role across communities and the causes and consequences of biodiversity loss. 相似文献
996.
Christian Kl?mbt 《Current biology : CB》2002,12(1):R21-R23
Recent studies have clarified how the active form of the Drosophila EGF receptor ligand Spitz is produced: Star chaperones Spitz in the ER and mediates its transport to the Golgi, where the intramembrane serine protease Rhomboid cleaves the Spitz proprotein to initiate secretion. 相似文献
997.
Lothar Heinisch Peter Gebhardt Renate Heidersbach Rolf Reissbrodt Ute Möllmann 《Biometals》2002,15(2):133-144
New analogues of triscatecholate siderophores based on linear or tripodal triamines with or without spacer groups or lipophilic and hydrophilic substituents were synthesized. The catecholate moieties were prepared in OH-forms, as acetylated compounds or masked as 8-methoxycarbonyloxy-2,4-dioxo-1,3-benzoxazine derivatives. Some of the new compounds were active as siderophores tested by growth promotion assays using various Gram-negative bacteria and mycobacteria under iron limitation and by CAS-assay. Structure-activity-correlations have been studied. 相似文献
998.
Susanne Vetterkind Hiroaki Miki Tadaomi Takenawa Ingrid Klawitz Karl-Heinz Scheidtmann Ute Preuss 《The Journal of biological chemistry》2002,277(1):87-95
We cloned and characterized the rat homologue of the Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP). Rat WIP shows 86% amino acid sequence identity to human WIP. Northern analyses revealed two major mRNA species of 5.0 and 3.8 kb, which were ubiquitously expressed, though predominantly in spleen and lung. Minor species of 2.4, 1.8, 1.4, and 1.1 kb were also detected in some tissues and cell lines. Thus, WIP is subject to tissue-specific alternative splicing. WIP bound to N-WASP in vivo, as revealed by co-immunoprecipitation. Expression of WIP in rat fibroblasts revealed a clear co-localization with actin stress fibers. However, expression in tumor cells lacking actin cables did not restore these structures. Interestingly, co-expression of WIP and N-WASP resulted in redistribution of N-WASP, abrogating its dominant nuclear expression and leading to co-localization with WIP in the perinuclear area and with actin in membrane protrusions. Moreover, stress fibers and, concomitantly, the associated WIP were largely dissolved. Very similar effects were seen upon epidermal growth factor stimulation of serum-starved cells. Our results suggest that WIP might be involved in transmitting mitogenic signals to cytoskeletal functions, perhaps by modulating the subcellular localization of N-WASP. Interaction of N-WASP with WIP may in turn lead to mobilization of actin from stress fibers and nucleation of new actin filaments in filopodia. 相似文献
999.
Martin Anlauf Rolf Eissele Martin K-H Sch?fer Lee E Eiden Rudolf Arnold Ursula Pauser Günter Kl?ppel Eberhard Weihe 《The journal of histochemistry and cytochemistry》2003,51(8):1027-1040
The uptake of monoamines into the secretory granules of monoamine-storing neuroendocrine cells is mediated by vesicular monoamine transporter protein 1 or 2 (VMAT1 or VMAT2). This study analyzed the expression of VMAT1 and VMAT2 in endocrine cells of normal human and monkey pancreas. The expression of VMAT1 and VMAT2 was also examined in infants with hyperinsulinemic hypoglycemia and in adults with pancreatic endocrine tumors (PETs). Using immunohistochemistry (IHC) and in situ hybridization (ISH), we demonstrated the mutually exclusive expression of VMAT1 in endocrine cells of the duct system and of VMAT2 in many cells of the islets of Langerhans. By confocal laser scanning microscopy, VMAT1-positive cells were identified as enterochromaffin (EC) cells and VMAT2-positive cells as beta-cells. In PETs, VMAT1 was found exclusively in all serotonin-containing tumors. In contrast, VMAT2 expression was lost in many insulinomas, independent of their biological behavior. VMAT2 was expressed by some non-insulin-producing tumors. The mutually exclusive expression of VMAT1 in EC cells and of VMAT2 in beta-cells suggests that both cell types store monoamines. Monoamine storage mediated by VMAT1 in EC cells is apparently maintained in EC cell tumors. In contrast, many insulinomas appear to lose their ability to accumulate monoamines via VMAT2. 相似文献
1000.