全文获取类型
收费全文 | 231篇 |
免费 | 17篇 |
专业分类
248篇 |
出版年
2021年 | 4篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 4篇 |
2017年 | 2篇 |
2016年 | 1篇 |
2015年 | 4篇 |
2014年 | 6篇 |
2013年 | 9篇 |
2012年 | 7篇 |
2011年 | 12篇 |
2010年 | 3篇 |
2009年 | 5篇 |
2008年 | 12篇 |
2007年 | 10篇 |
2006年 | 11篇 |
2005年 | 10篇 |
2004年 | 9篇 |
2003年 | 11篇 |
2002年 | 13篇 |
2001年 | 9篇 |
2000年 | 10篇 |
1999年 | 6篇 |
1998年 | 2篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 12篇 |
1991年 | 2篇 |
1990年 | 6篇 |
1989年 | 3篇 |
1988年 | 5篇 |
1987年 | 2篇 |
1986年 | 6篇 |
1985年 | 7篇 |
1984年 | 7篇 |
1983年 | 5篇 |
1982年 | 1篇 |
1981年 | 3篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 5篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1967年 | 2篇 |
1965年 | 1篇 |
排序方式: 共有248条查询结果,搜索用时 0 毫秒
71.
72.
Serum amyloid A (SAA)-induced remodeling of CSF-HDL 总被引:2,自引:0,他引:2
Miida T Yamada T Seino U Ito M Fueki Y Takahashi A Kosuge K Soda S Hanyu O Obayashi K Miyazaki O Okada M 《Biochimica et biophysica acta》2006,1761(4):424-433
Inflammation is a risk factor for Alzheimer's disease. Serum amyloid A (SAA) is an acute phase protein that dissociates apolipoprotein AI (apoAI) from plasma HDL. In cerebrospinal fluid (CSF), the SAA concentration is much higher in subjects with Alzheimer's disease than in controls. CSF-HDL is rich in apoE, which plays an important role as a ligand for lipoprotein receptors in the central nervous system (CNS). To clarify whether SAA dissociates apoE from CSF-HDL, we added recombinant SAA to CSF and determined the apoE distribution in the CSF using native two-dimensional gel electrophoresis. We found that SAA dissociated apoE from CSF-HDL in a dose-dependent manner. This effect was more evident in apoE4 carriers than in apoE3 or apoE2 carriers. After a 24-h incubation at 37 degrees C, SAA continuously dissociated apoE from CSF-HDL. Amyloid beta (Abeta) fragments (1-42) were bound to large CSF-HDL but not to apoE dissociated by SAA. In conclusion, SAA dissociates apoE from CSF-HDL. We postulate that inflammation in the CNS may impair Abeta clearance due to the loss of apoE from CSF-HDL. 相似文献
73.
An autocrine function of nerve growth factor for cell cycle regulation of vascular endothelial cells 总被引:13,自引:0,他引:13
Tanaka A Wakita U Kambe N Iwasaki T Matsuda H 《Biochemical and biophysical research communications》2004,313(4):1009-1014
Nerve growth factor (NGF) regulates maintenance, survival, and function of not only neuronal cells but also various kinds of non-neuronal cells. Here we clearly demonstrated that mouse aortic endothelial cells (AEC) produced bioactive NGF, and the production was enhanced by a proinflammatory cytokine, interleukin (IL)-1beta. AEC expressed both high affinity (TrkA) and low affinity (p75(NGFR)) receptors for NGF. Exogenously added NGF induced rapid phosphorylation of TrkA tyrosine kinase. Addition of anti-NGF neutralizing antibody resulted in an increase in the proportion of AEC in S and G(2)/M phases and in a hypodiploid range. Since the vascular endothelium plays a pivotal role in inflammatory conditions, these results strongly suggest that NGF, whose production is enhanced at the affected site, may contribute to maintenance, survival, and function of vascular endothelial cells by autocrine and/or paracrine mechanisms. 相似文献
74.
75.
Suzuki M Mizoguchi M Yano F Hara U Yokoyama M Watanabe N 《Zeitschrift für Naturforschung. C, Journal of biosciences》2003,58(3-4):220-224
We investigated the effects of catecholamine on flower-induction in P. nil (cv. Violet). GC-SIM analysis identified dopamine for the first time in P. nil seedlings. Dopamine levels in the cotyledons did not show a significant change during the inducing dark treatment. The dopamine content of cotyledons exposed to various durations of darkness were 0.1-0.2 nmol/g fresh weight. The same content was found when cotyledons were exposed to continuous light. 相似文献
76.
Cross-priming of diabetogenic T cells dissociated from CTL-induced shedding of beta cell autoantigens 总被引:4,自引:0,他引:4
Yamanouchi J Verdaguer J Han B Amrani A Serra P Santamaria P 《Journal of immunology (Baltimore, Md. : 1950)》2003,171(12):6900-6909
Cross-presentation of self Ags by APCs is key to the initiation of organ-specific autoimmunity. As MHC class I molecules are essential for the initiation of diabetes in nonobese diabetic (NOD) mice, we sought to determine whether the initial insult that allows cross-presentation of beta cell autoantigens in diabetes is caused by cognate interactions between naive CD8(+) T cells and beta cells. Naive splenic CD8(+) T cells from transgenic NOD mice expressing a diabetogenic TCR killed peptide-pulsed targets in the absence of APCs. To ascertain the role of CD8(+) T cell-induced beta cell lysis in the initiation of diabetes, we expressed a rat insulin promoter (RIP)-driven adenovirus E19 transgene in NOD mice. RIP-E19 expression inhibited MHC class I transport exclusively in beta cells and rendered these cells resistant to lysis by CD8(+) (but not CD4(+)) T cells, both in vitro and in vivo. Surprisingly, RIP-E19 expression impaired the accumulation of CD8(+) T cells in islets and delayed the onset of islet inflammation, without affecting the timing or magnitude of T cell cross-priming in the pancreatic lymph nodes, which is the earliest known event in diabetogenesis. These results suggest that access of beta cell autoantigens to the cross-presentation pathway in diabetes is T cell independent, and reveal a previously unrecognized function of MHC class I molecules on target cells in autoimmunity: local retention of disease-initiating clonotypes. 相似文献
77.
Kanai T Totsuka T Uraushihara K Makita S Nakamura T Koganei K Fukushima T Akiba H Yagita H Okumura K Machida U Iwai H Azuma M Chen L Watanabe M 《Journal of immunology (Baltimore, Md. : 1950)》2003,171(8):4156-4163
A newly identified costimulatory molecule, programmed death-1 (PD-1), provides a negative signal that is essential for immune homeostasis. However, it has been suggested that its ligands, B7-H1 (PD-L1) and B7-dendritic cells (B7-DC; PD-L2), could also costimulate T cell proliferation and cytokine secretion. Here we demonstrate the involvement of PD-1/B7-H1 and B7-DC interaction in the development of colitis. We first examined the expression profiles of PD-1 and its ligands in both human inflammatory bowel disease and a murine chronic colitis model induced by adoptive transfer of CD4(+)CD45RB(high) T cells to SCID mice. Second, we assessed the therapeutic potential of neutralizing anti-B7-H1 and/or B7-DC mAbs using this colitis model. We found significantly increased expression of PD-1 on T cells and of B7-H1 on T, B, and macrophage/DCs in inflamed colon from both inflammatory bowel disease patients and colitic mice. Unexpectedly, the administration of anti-B7-H1, but not anti-B7-DC, mAb after transfer of CD4(+)CD45RB(high) T cells suppressed wasting disease with colitis, abrogated leukocyte infiltration, and reduced the production of IFN-gamma, IL-2, and TNF-alpha, but not IL-4 or IL-10, by lamina propria CD4(+) T cells. These data suggest that the interaction of PD-1/B7-H1, but not PD-1/B7-DC, might be involved in intestinal mucosal inflammation and also show a possible role of interaction between B7-H1 and an as yet unidentified receptor for B7-H1 in inducing T cell activation. 相似文献
78.
Keiko Horiuchi Atsushi Takatori Toshiaki Inenaga Etsuko Ohta Jun Yamanouchi Seiji Kawamura Yoshiyuki Ishii Shigeru Kyuwa Yasuhiro Yoshikawa 《Experimental Animals》2002,51(5):457-464
We investigated the effect of probucol (PB) on atherosclerosis in streptozotocin (SZ)-induced diabetic-hyperlipidemic APA hamsters in three different stages, the early, middle and late stages of atherosclerosis. Male APA hamsters were injected intraperitoneally with SZ or vehicle alone (citrate buffer; CB) as a control at the age of 8 weeks. At 6 weeks after injection (WAI) of SZ or CB (the early stage), 14 WAI (the middle stage) and 26 WAI (the late stage), animals were assigned to PB treated- or non-treated groups (CBPB, SZPB, CB, SZ). After 8 weeks of PB administration with diet, the aorta was taken from each animal for assessment of atheromatous lesions and blood samples were subjected to serum biochemical analysis and the measurement of blood lipid peroxide (LPO). In the middle stage, PB treatment significantly decreased serum total cholesterol level, slightly decreased LPO, and also tended to reduce the lesion area, although no statistical difference was seen. There was no marked effect of PB treatment in the early and late stages. These findings suggest that single use of PB has little effect on atherosclerosis of a hyperglycemia-hyperlipidemia animal model. 相似文献
79.
Assignment of human β-galactosidase-A gene to 3p21.33 by fluorescence in situ hybridization 总被引:29,自引:0,他引:29
GM1 gangliosidosis and Morquio syndrome type B (MPS IVB) are inherited lyosomal storage disorders associated with deficiency of -galactosidase-A (GALA) activity. A recombinant plasmid containing a biotinylated cDNA (2.4-kb insert) encoding human GALA was used to localize the enzyme locus by fluorescence in situ hybridization (FISH). The human GALA gene was assigned to 3p21.33 by FISH. 相似文献
80.
Shuichi Kikuchi Yasuko Yamanouchi Liming Li Kimiko Kobayashi Hiroshi Ijima Ryunosuke Miyazaki Shigeru Tsuchiya Hideo Hamaguchi 《Human genetics》1992,90(1-2):7-11
Summary A functionally inactive plasminogen (PLG) variant designated as PLG M5 is polymorphic in the Japanese population and has a feature common to PLG with type-I mutation that has a codon 601 missense mutation in exon 15 (GCT for AlaACT for Thr). This study was conducted to clarify whether the type-I mutation of PLG is present in PLG M5 and polymorphic in the Japanese population. Direct sequencing of the amplified DNA from the PLG gene in a heterozygote for PLG M5 revealed that the sequence of the exon 15 in the gene for PLG M5 is identical with that in the PLG gene with type-I mutation. In addition, the amplified DNA from the PLG gene in 12 heterozyotes for PLG M5 reacted with the probe for the type-I mutation in dot blot hybridization with an allele-specific oligonucleotide probe. The heterozygote for PLG with type-I mutation was found in 2.2% of 360 unrelated healthy subjects. These data indicate that the type-I mutation of PLG is present in PLG M5 and polymorphic in the Japanese population. The data also suggest that the PLG M5 is identical with PLG Tochigi and Kagoshima. 相似文献