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101.
Changing concepts in plant hormone action 总被引:4,自引:0,他引:4
Th.?GasparEmail author C.?Kevers O.?Faivre-Rampant M.?Crèvecoeur CL.?Penel H.?Greppin J.?Dommes 《In vitro cellular & developmental biology. Plant》2003,39(2):85-106
Summary A plant hormone is not, in the classic animal sense, a chemical synthesized in one organ, transported to a second organ to
exert a chemical action to control a physiological event. Any phytohormone can be synthesized everywhere and can influence
different growth and development processes at different places. The concept of physiological activity under hormonal control
cannot be dissociated from changes in concentrations at the site of action, from spatial differences and changes in the tissue's
sensitivity to the compound, from its transport and its metabolism, from balances and interactions with the other phytohormones,
or in their metabolic relationships, and in their signaling pathways as well. Secondary messengers are also involved. Hormonal
involvement in physiological processes can appear through several distinct manifestations (as environmental sensors, homeostatic
regulators and spatio-temporal synchronizers, resource allocators, biotime adjusters, etc.), dependent on or integrated with
the primary biochemical pathways. The time has also passed for the hypothesized ‘specific’ developmental hormones, rhizocaline,
canlocaline, and florigen: root, stem, and flower formation result from a sequential control of specific events at the right
places through a coordinated control by electrical signals, the known phytohormones and nonspecific molecules of primary and
secondary metabolism, and involve both cytoplasmic and apoplastic compartments. These contemporary views are examined in this
review. 相似文献
102.
Cell suspension cultures are useful for a wide range of biochemical and physiological studies, yet their production can be
technically demanding and often unreliable. Here we describe a protocol for producing Arabidopsis cell suspension cultures
that is reliable and easy to use. 相似文献
103.
PHGPx and spermatogenesis 总被引:2,自引:0,他引:2
PHGPx of rat sperm mitochondrial capsule is cross-linked and inactive. The enzyme is in part released in an active form by mercaptoethanol. Treatment with H(2)O(2) of reduced and solubilised capsule proteins, in the absence of any added reductant, results in: i) H(2)O(2) consumption which depends on the presence of both, PHGPx activity and protein thiols; ii) protein thiol oxidation with a stoichiometry of 2 equivalents of thiol per mole of hydroperoxide and, iii) PHGPx inactivation and cross-linking. SDS-PAGE analysis of monobromobimane-labeled proteins, following incubation with H(2)O(2), shows that the oxidation takes place in specific bands in the area of 20~kDa. It is concluded that the protein thiol peroxidase activity of PHGPx is responsible for cross-linking proteins in the mammalian sperm capsule and accounts for the selenium dependency of spermatogenesis. 相似文献
104.
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107.
The sequence of 967 amino acids at the carboxyl-end of rat thyroglobulin. Location and surroundings of two thyroxine-forming sites 总被引:7,自引:0,他引:7
R Di Lauro S Obici D Condliffe V M Ursini A Musti C Moscatelli V E Avvedimento 《European journal of biochemistry》1985,148(1):7-11
The entire rat thyroglobulin mRNA sequence (about 8500 nucleotides) has been cloned in five recombinant plasmids containing overlapping cDNA inserts. The 3' end of the mRNA is precisely defined by the poly (A) tail found in the furthest 3' end clone. Evidence that most of the 5' end is cloned come from size considerations and from a primer extension experiment. At the 3' end of the mRNA only one long open reading frame is present in the sequence of 3018 nucleotides that has been established. In the deduced protein sequence we have localized two thyroxine-forming sites in a region containing a high concentration of tyrosine residues. 相似文献
108.
The role of selenium peroxidases in the protection against oxidative damage of membranes 总被引:7,自引:0,他引:7
The present review deals with the chemical properties of selenium in relation to its antioxidant properties and its reactivity in biological systems. The interaction of selenite with thiols and glutathione and the reactivity of selenocompounds with hydroperoxides are described. After a short survey on distribution, metabolism and organification of selenium, the role of this element as a component of the two seleno-dependent glutathione peroxidases is described. The main features of glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase are also reviewed. Both enzymes reduce different hydroperoxides to the corresponding alcohols and the major difference is the reduction of lipid hydroperoxides in membrane matrix catalyzed only by the phospholipid hydroperoxide glutathione peroxidase. However, in spite of the different specificity for the peroxidic substrates, the kinetic mechanism of both glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase seems identical and proceeds through a tert-uni ping pong mechanism. In the reaction cycle, indeed, as supported by the kinetic data, the oxidation of the ionized selenol by the hydroperoxide yields a selenenic acid that in turn is reduced back by two reactions with reduced glutathione. Special emphasis has been given to the role of selenium-dependent glutathione peroxidases in the prevention of membrane lipid peroxidation. While glutathione peroxidase is able to reduce hydrogen peroxide and other hydroperoxides possibly present in the soluble compartment of the cell, this enzyme fails to inhibit microsomal lipid peroxidation induced by NADPH or ascorbate and iron complexes. On the other hand, phospholipid hydroperoxide glutathione peroxidase, by reducing the phospholipid hydroperoxides in the membranes, actively prevents lipid peroxidation, provided a normal content of vitamin E is present in the membranes. In fact, by preventing the free radical generation from lipid hydroperoxides, phospholipid hydroperoxide glutathione peroxidase decreases the vitamin E requirement necessary to inhibit lipid peroxidation. Finally, the possible regulatory role of the selenoperoxidases on the arachidonic acid cascade enzymes (cyclooxygenase and lipoxygenase) is discussed. 相似文献
109.
110.
Protein binding domains of the rat thyroglobulin promoter 总被引:2,自引:0,他引:2
M V Ursini A Gallo E Olivetta A M Musti 《Biochemical and biophysical research communications》1989,163(1):481-488