排序方式: 共有42条查询结果,搜索用时 78 毫秒
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Ryan F. Donnelly Maelíosa T. C. McCrudden Ahlam Zaid Alkilani Eneko Larra?eta Emma McAlister Aaron J. Courtenay Mary-Carmel Kearney Thakur Raghu Raj Singh Helen O. McCarthy Victoria L. Kett Ester Caffarel-Salvador Sharifa Al-Zahrani A. David Woolfson 《PloS one》2014,9(10)
We describe, for the first time, hydrogel-forming microneedle arrays prepared from “super swelling” polymeric compositions. We produced a microneedle formulation with enhanced swelling capabilities from aqueous blends containing 20% w/w Gantrez S-97, 7.5% w/w PEG 10,000 and 3% w/w Na2CO3 and utilised a drug reservoir of a lyophilised wafer-like design. These microneedle-lyophilised wafer compositions were robust and effectively penetrated skin, swelling extensively, but being removed intact. In in vitro delivery experiments across excised neonatal porcine skin, approximately 44 mg of the model high dose small molecule drug ibuprofen sodium was delivered in 24 h, equating to 37% of the loading in the lyophilised reservoir. The super swelling microneedles delivered approximately 1.24 mg of the model protein ovalbumin over 24 h, equivalent to a delivery efficiency of approximately 49%. The integrated microneedle-lyophilised wafer delivery system produced a progressive increase in plasma concentrations of ibuprofen sodium in rats over 6 h, with a maximal concentration of approximately 179 µg/ml achieved in this time. The plasma concentration had fallen to 71±6.7 µg/ml by 24 h. Ovalbumin levels peaked in rat plasma after only 1 hour at 42.36±17.01 ng/ml. Ovalbumin plasma levels then remained almost constant up to 6 h, dropping somewhat at 24 h, when 23.61±4.84 ng/ml was detected. This work represents a significant advancement on conventional microneedle systems, which are presently only suitable for bolus delivery of very potent drugs and vaccines. Once fully developed, such technology may greatly expand the range of drugs that can be delivered transdermally, to the benefit of patients and industry. Accordingly, we are currently progressing towards clinical evaluations with a range of candidate molecules. 相似文献
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The monitoring of an experimental feeding station established in northern Spain allowed the evaluation of how this type of resource, predictable in space but not in time, was exploited by a guild of avian scavengers in relation to factors such as season, hour of disposal and presence of the dominant species. The presence of Egyptian Vultures Neophron percnopterus at carcasses was more likely during spring, and richness and diversity of avian scavengers was lower during the summer and when Griffon Vultures Gyps fulvus arrived earlier. The temporal unpredictability of the resource may favour exploitation by smaller and less competitive scavengers. New European regulations may present an opportunity to develop effective conservation measures to support functional scavenger assemblages. 相似文献
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José A. Donázar Ainara Cortés-Avizanda Eneko Arrondo Alejandro Delgado-González Olga Ceballos 《Ibis》2023,165(1):305-311
Protected areas in southern Europe are important for the conservation of large avian scavengers. However, the effects an increasing number of visitors may have on the scavengers' patterns of movement are unknown. Here, we took advantage of data collected from seven GPS-tagged adult Griffon Vultures Gyps fulvus breeding in the Bardenas Reales Natural Park in northern Spain to determine whether foraging birds moved to more remote areas on the days when the number of visitors increased. We found that although the number of visitors did appear to affect movement patterns, this had a smaller effect size compared with the mean temperature of the day. Additionally, males moved further than females. If the number of visitors to natural areas continues to increase, local exclusions of Griffon Vultures may become more common, so further research is needed to address the potential consequences for the scavenger population and ecosystem functions and services they provide. 相似文献
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Quantitative trait loci controlling vegetative growth rate in the edible basidiomycete Pleurotus ostreatus 总被引:2,自引:0,他引:2
Larraya LM Idareta E Arana D Ritter E Pisabarro AG Ramírez L 《Applied and environmental microbiology》2002,68(3):1109-1114
Mycelium growth rate is a quantitative characteristic that exhibits continuous variation. This trait has applied interest, as growth rate is correlated with production yield and increased advantage against competitors. In this work, we studied growth rate variation in the edible basidiomycete Pleurotus ostreatus growing as monokaryotic or dikaryotic mycelium on Eger medium or on wheat straw. Our analysis resulted in identification of several genomic regions (quantitative trait loci [QTLs]) involved in the control of growth rate that can be mapped on the genetic linkage map of this fungus. In some cases monokaryotic and dikaryotic QTLs clustered at the same map position, indicating that there are principal genomic areas responsible for growth rate control. The availability of this linkage map of growth rate QTLs can help in the design of rational strain breeding programs based on genomic information. 相似文献
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Javier Carmona Jiménez Angela Caro Borrero Itzel Becerra-Absalón Elvira Perona Urizar Kenia Márquez Santamaría Pilar Mateo Ortega 《Journal of phycology》2023,59(6):1237-1257
The present study describes two new Nostoc species, N. montejanii and N. tlalocii, based on a polyphasic approach that combines morphological, ecological, and genetic characteristics. The five investigated populations, including those from newly collected material from central Mexico, were observed to possess morphological features characteristic of the Nostoc genus. Results showed that both new species are strictly associated with running water, and they show clear differences in their habitat preferences. The 16S rRNA gene sequences of the five strains displayed between 98% and 99% similarity to the genus Nostoc sensu stricto. The 16S rRNA gene phylogenetic analyses inferred using Bayesian inference, maximum likelihood, and parsimony methods, placed these five strains in two separate clades distinct from other Nostoc species. The secondary structures of the 16S–23S internal transcribed spacer rRNA region in the two new species showed >10.5% dissimilarities in the operons when compared with other Nostoc species. In addition, clear morphological differences were observed between the two Mexican species, including the color of the colonies (black in N. montejanii and green in N. tlalocii), the size of the cells (greater in N. montejanii), and the number of polyphosphate granules present in the cells (one in N. montejanii and up to four in N. tlalocii). 相似文献
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The Norwegian spring-spawning (NSS) herring (Clupea harengus), blue whiting (Micromesistius poutassou) and Northeast Atlantic (NEA) mackerel (Scomber scombrus) are extremely abundant pelagic planktivores that feed in the Norwegian Sea (NS) during spring and summer. This study investigated the feeding ecology and diet composition of these commercially important fish stocks on the basis of biological data, including an extensive set of stomach samples in combination with hydrographical data, zooplankton samples and acoustic abundance data from 12 stock monitoring surveys carried out in 2005–2010. Mackerel were absent during the spring, but had generally high feeding overlap with herring in the summer, with a diet mainly based on calanoid copepods, especially Calanus finmarchicus, as well as a similar diet width. Stomach fullness in herring diminished from spring to summer and feeding incidence was lower than that of mackerel in summer. However, stomach fullness did not differ between the two species, indicating that herring maintain an equally efficient pattern of feeding as mackerel in summer, but on a diet that is less dominated by copepods and is more reliant on larger prey. Blue whiting tended to have a low dietary overlap with mackerel and herring, with larger prey such as euphausiids and amphipods dominating, and stomach fullness and feeding incidence increasing with length. For all the species, feeding incidence increased with decreasing temperature, and for mackerel so did stomach fullness, indicating that feeding activity is highest in areas associated with colder water masses. Significant annual effects on diet composition and feeding-related variables suggested that the three species are able to adapt to different food and environmental conditions. These annual effects are likely to have an important impact on the predation pressure on different plankton groups and the carrying capacity of individual systems, and emphasise the importance of regular monitoring of pelagic fish diets. 相似文献
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Esther Sebastián-González Jon Morant Marcos Moleón Daniel Redondo-Gómez Zebensui Morales-Reyes Roberto Pascual-Rico Juan Manuel Pérez-García Eneko Arrondo 《Global Ecology and Biogeography》2023,32(8):1302-1310