全文获取类型
收费全文 | 189篇 |
免费 | 8篇 |
专业分类
197篇 |
出版年
2022年 | 4篇 |
2021年 | 10篇 |
2020年 | 3篇 |
2019年 | 2篇 |
2018年 | 4篇 |
2016年 | 7篇 |
2015年 | 8篇 |
2014年 | 16篇 |
2013年 | 12篇 |
2012年 | 7篇 |
2011年 | 8篇 |
2010年 | 6篇 |
2009年 | 8篇 |
2008年 | 3篇 |
2007年 | 4篇 |
2005年 | 6篇 |
2003年 | 3篇 |
2002年 | 5篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 5篇 |
1991年 | 1篇 |
1990年 | 4篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 2篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 2篇 |
1974年 | 7篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1971年 | 4篇 |
1970年 | 1篇 |
1969年 | 2篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1964年 | 1篇 |
1963年 | 2篇 |
排序方式: 共有197条查询结果,搜索用时 15 毫秒
61.
José Uriel Hans Kristian Lorenzo Antonio Macho Adrian W. de Feijter Zohar Mishal 《Histochemistry and cell biology》1995,103(5):355-361
We have employed a laser scanning confocal microscope in reflection mode to directly and indirectly visualize sites of deposition of silver-enhanced reaction products from colloidal gold probes. A direct approach was used for the localization of alpha-fetoprotein receptors in human myoblasts by incubating primary cultures with an alpha-fetoprotein-gold conjugate. For an indirect approach, cultured CEM cells, derived from a human T-lymphoma cell line, were incubated with a mouse monoclonal antibody to mature T-cells, followed by a gold-labelled antibody to mouse immunglobulins. Multiple optical sections of each sample were collected by reflection laser scanning confocal microscopy and combined into three-dimensional renderings. A (non-confocal) transmission image was generated of each field for comparative purposes. The increasing use of reflection laser scanning confocal microscopy combined with colloidal gold conjugates as biological markes will probably be of considerable advantage in cytochemical analysis. 相似文献
62.
63.
A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of 14CO2 from [14C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved 14CO2 is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of incubation. Coliform bacteria suspended in water for several days recover from starvation and may be quantitated by the proposed method. Bacteria from water samples may also be concentrated by filtration through membrane filters and detected by the radiometric assay. 相似文献
64.
65.
A covalent conjugate of alpha-foetoprotein (AFP) and horseradish peroxidase (HRP) has been used to follow, at the ultrastructural level, the pathway of AFP uptake and translocation in a rat rhabdomyosarcoma cell line. The cells were incubated for several times at 4 degrees C and/or 37 degrees C, and fixed. AFP-HRP was found to enter the cells via coated pits and receptosomes and to move to tubular elements of the trans-reticular portion of the Golgi. Some observations suggest that AFP can be recycled back to the cell surface. On the other hand, the cells were incubated with a noncovalent conjugate of AFP and 3H-arachidonic acid [3H-(20:4)], and the uptake of the fatty acid molecules studied by ultrastructural autoradiography. The cytoplasmic labeling, very low after an incubation in the presence of [3H-(20:4)]-AFP for 2 hours at 4 degrees C, increased rapidly after transfer of the cells for 5 minutes to 37 degrees C. These observations support the hypothesis that AFP plays a role in the intracellular delivery of polyunsaturated fatty acids. 相似文献
66.
67.
68.
69.
70.