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排序方式: 共有1365条查询结果,搜索用时 15 毫秒
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Marsault E Hoveyda HR Gagnon R Peterson ML Vézina M Saint-Louis C Landry A Pinault JF Ouellet L Beauchemin S Beaubien S Mathieu A Benakli K Wang Z Brassard M Lonergan D Bilodeau F Ramaseshan M Fortin N Lan R Li S Galaud F Plourde V Champagne M Doucet A Bhérer P Gauthier M Olsen G Villeneuve G Bhat S Foucher L Fortin D Peng X Bernard S Drouin A Déziel R Berthiaume G Dory YL Fraser GL Deslongchamps P 《Bioorganic & medicinal chemistry letters》2008,18(16):4731-4735
A new method for solid phase parallel synthesis of chemically and conformationally diverse macrocyclic peptidomimetics is reported. A key feature of the method is access to broad chemical and conformational diversity. Synthesis and mechanistic studies on the macrocyclization step are reported. 相似文献
195.
A. I. Bhat P. S. Hareesh R. Madhubala 《Journal of plant biochemistry and biotechnology.》2005,14(1):37-40
The coat protein gene of an isolate of Cucumber mosaic virus (CMV) associated with stunted disease affecting black pepper plant was cloned and sequenced. The coat protein gene comprised of 657 nucleotides encoding a protein of 218 amino acids. Sequence analysis showed that the gene was most closely related to CMV infecting Egyptian henbane plant in India, the member of subgroup I of CMV. The amino acid sequence identity with members of subgroup I was 92-99% while that with members of subgroup II was 77–79%. On the basis of sequence homology, it is concluded that CMV infecting black pepper in India is a strain of CMV belonging to subgroup I. 相似文献
196.
John Westbrook Zukang Feng Shri Jain T. N. Bhat Narmada Thanki Veerasamy Ravichandran Gary L. Gilliland Wolfgang F. Bluhm Helge Weissig Douglas S. Greer Philip E. Bourne Helen M. Berman 《Nucleic acids research》2002,30(1):245-248
The Protein Data Bank (PDB; http://www.pdb.org/) is the single worldwide archive of structural data of biological macromolecules. This paper describes the progress that has been made in validating all data in the PDB archive and in releasing a uniform archive for the community. We have now produced a collection of mmCIF data files for the PDB archive (ftp://beta.rcsb.org/pub/pdb/uniformity/data/mmCIF/). A utility application that converts the mmCIF data files to the PDB format (called CIFTr) has also been released to provide support for existing software. 相似文献
197.
Synthesis of nucleic acid probes on membrane supports: a procedure for the removal of unincorporated precursors 总被引:1,自引:0,他引:1
S P Bhat 《Analytical biochemistry》1990,184(1):59-62
We have used DNA bound to small pieces of nylon membrane for the synthesis of radioactive probes. The DNA to be used for generating the probe(s) is first bound to nylon membranes and then introduced into the reaction mix. The labeling reaction takes place on the membrane and therefore allows easy removal of unincorporated precursors by simple washing for 1-2 min. The clean labeled probe is eluted from the membrane in formamide or in water and is ready for use. This DNA-membrane can be stored for reuse. Synthesis of probes on a solid support such as nylon membrane thus circumvents problems associated with chromatographic manipulations needed for the separation of labeled DNA from unicorporated precursors. Probes synthesized in this manner are as efficient in detecting nucleic acid sequences as those synthesized in solution. 相似文献
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Dipnarayan Saha Vajinder Kumar Shripad Ramachandra Bhat Ramamurthy Srinivasan 《Plant Molecular Biology Reporter》2011,29(2):265-277
Isolation and characterization of promoters are important in understanding gene regulation and genetic engineering of crop
plants. Earlier, a pentatricopeptide repeat protein (PPR) encoding gene (At2g39230), designated as Lateral Organ Junction (LOJ) gene, was identified through T-DNA promoter trapping in Arabidopsis thaliana. The upstream sequence of the LOJ gene conferred on the reporter gene a novel LOJ-specific expression. The present study was aimed at identifying and characterizing
the cis-regulatory motifs responsible for tissue-specific expression in the −673 and +90 bases upstream of the LOJ gene recognized as LOJ promoter. In silico analysis of the LOJ promoter revealed the presence of a few relevant regulatory motifs and a unique feature like AT-rich inverted repeat. Deletion
analysis of the LOJ promoter confirmed the presence of an enhancer-like element in the distal region (−673/−214), which stimulates a minimal
promoter-like sequence in the −424/−214 region in a position and orientation autonomous manner. The −136/+90 region of the
LOJ promoter was efficient in driving reporter gene expression in tissues like developing anthers and seeds of Arabidopsis. A positive regulation for the seed- and anther-specific expression module was contemplated within the 5′ untranslated region
of the LOJ gene. However, this function was repressed in the native context by the lateral organ junction-specific expression. The present
study has led to the identification of a novel lateral organ junction-specific element and an enhancer sequence in Arabidopsis with potential applications in plant genetic engineering. 相似文献
200.
E. Rai S. Sharma A. Koul A. K. Bhat A. J. S. Bhanwer R. N. K. Bamezai 《Human genetics》2008,123(1):117