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G. HOLSBEEK J. MERGEAY H. HOTZ J. PLÖTNER F. A. M. VOLCKAERT L. DE MEESTER 《Molecular ecology》2008,17(23):5023-5035
In Western Europe, many pond owners introduce amphibians for ornamental purposes. Although indigenous amphibians are legally protected in most European countries, retailers are circumventing national and international legislation by selling exotic nonprotected sibling species. We investigated to what extent non‐native species of the European water frog complex (genus Pelophylax) have become established in Belgium, using morphological, mitochondrial and nuclear genetic markers. A survey of 87 sampling sites showed the presence of non‐native water frogs at 47 locations, mostly Marsh frogs (Pelophylax ridibundus). Surprisingly, at least 19% of all these locations also harboured individuals with mitochondrial haplotypes characteristic of Anatolian water frogs (Pelophylax cf. bedriagae). Nuclear genotyping indicated widespread hybridization and introgression between P. ridibundus and P. cf. bedriagae. In addition, water frogs of Turkish origin obtained through a licensed retailer, also contained P. ridibundus and P. cf. bedriagae, with identical haplotypes to the wild Belgian populations. Although P. ridibundus might have invaded Belgium by natural range expansion from neighbouring countries, our results suggest that its invasion was at least partly enhanced by commercial trade, with origins as far as the Middle East. Also the invasion and rapid spread of Anatolian lineages, masked by their high morphological similarity to P. ridibundus, is likely the result of unregulated commercial trade. We expect that Anatolian frogs will further invade the exotic as well as the native range of P. ridibundus and other Pelophylax species elsewhere in Western and Central Europe, with risks of large‐scale hybridization and introgression. 相似文献
63.
外源氮输入和水分变化对荒漠草原凋落物分解的影响 总被引:1,自引:0,他引:1
全球气候变化背景下,大气氮沉降和降水变化日益显著,其对荒漠草原凋落物分解的影响存在很大的不确定性.采用裂区设计,设置主区为自然降雨、增雨30%和减雨30% 3个水分处理,副区为0(N0)、30(N30)、50(N50)和100 kg·hm-2·a-1(N100)4个氮素水平,经过21个月(2016年1月—2017年10月)水氮处理,研究水氮共同作用对荒漠草原常见物种猪毛菜、短花针茅和木地肤3种植物凋落物分解的影响.结果表明: 3种凋落物干物质残留率随时间增加而减少,用Olson负指数衰减模型拟合效果较好,凋落物分解系数(k)大小为猪毛菜>短花针茅>木地肤.增雨30%N100处理分解系数最高,为0.028.单因素处理下,增雨30%和N50的凋落物分解最快.水氮共同作用下,增雨 30%N100处理凋落物分解最快.3种凋落物初始化学全氮含量大小为猪毛菜>短花针茅>木地肤,猪毛菜和短花针茅k值与全氮含量呈显著正相关;全碳含量、纤维素含量、木质素含量、C/N、木质素/N和纤维素/N大小为木地肤>短花针茅>猪毛菜,猪毛菜k值与各指标均呈显著负相关,短花针茅和木地肤k值与C/N、木质素/N和纤维素/N均呈显著负相关.猪毛菜分解最快,木地肤分解最慢.适量的水、氮添加有利于荒漠草原凋落物的分解,可以促进土壤养分循环,对荒漠草原可持续发展及生态平衡有积极作用. 相似文献
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THE ENUMERATION OF FAECAL STREPTOCOCCI IN FOODS, USING PACKER'S CRYSTAL VIOLET SODIUM AZIDE BLOOD AGAR 总被引:3,自引:3,他引:0
D. A. A. MOSSEL H. M. J. VAN DIEPEN A. S. DE BRUIN 《Journal of applied microbiology》1957,20(2):265-272
SUMMARY: Packer's crystal violet sodium azide blood agar (Packer, 1943) used in poured plates at 36·1°, gave satisfactory recovery of pure cultures of Lancefield group D streptococci and completely inhibited the growth of 11 other species of aerobic and anaerobic food bacteria, including Strep. lactis (5 strains). Later, however, one group N streptococcus was obtained which did grow in Packer's agar at 36·1°. To eliminate this organism the incubation temperature had to be increased to 39·5°, using agar strips (Stirling et al. 1950) incubated in a water bath to secure strict temperature control. Under these conditions the recovery of typical group D streptococci was never consistently below 50% of the count in tryptone dextrose yeast extract agar at 31·1°. 相似文献
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ARNOLD DE LOOF 《Insect Science》2006,13(5):325-338
The paradigm, still around in textbooks, that 'in insects sex is strictly genetic, thus that they do not have sex hormones', is mainly based on a wrong interpretation of the 'gynandromorph argument'. It is no longer tenable. Given the fact that vertebrates and invertebrates probably had a common, sexually reproducing ancestor, there is no reason to assume that only vertebrates need sex hormones. The major function of sex hormones is to inform the somatoplasm about developmental changes that take place in the gonads. In contrast to juvenile hormone and neuropeptides, ecdysteroids meet all criteria to act as sex hormones, which was probably their ancient role. Their much better documented role in moulting and metamorphosis was a secondary acquisition that enabled arthropods to cope with growth problems, imposed by a rigid cuticle. Female insects use 20-hydroxyecdysone (20E), secreted by the follicle cells of the ovary, in a similar way as females of egg-laying vertebrates use estrogens. For a variety of reasons, the possibility that ecdysteroids, in particular ecdysone (E), might also act as sex hormones in male insects, thus as the counterpart of testosterone of vertebrates, has been very much overlooked. Thanks to the recent discovery of the molecular basis of the haploid-diploid system of sex determination in the honeybee, the characterization of Halloween genes, proteomics, RNAi and so on, it now becomes possible to verify whether in insects, as with vertebrates, males are the endocrinologically default gender form. 相似文献
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为依据春尺蠖Apocheima cinerarius Erschoff蛹的形态特征快速、无损、准确鉴别雌雄个体,对其头部、胸部、腹部和体色外部鉴别特征进行了分析,并通过解剖生殖系统验证准确性.结果表明:以春尺蠖蛹的胸部和腹部特征识别雌雄准确率明显高于头部和体色特征,识别率可达100%.首先,雌蛹第8腹节腹板前缘中部具有"Y"型沟,与第7腹节腹板后缘形成倒三角状,而雄蛹无此特征.其次,雌蛹生殖孔与产卵孔连接形成裂缝,两侧平坦无突起,而雄蛹第9腹节腹板中央有一纵裂缝的生殖孔,两侧各有半圆状瘤状突起.最后,雌蛹的胸部背板各节间相对长度均小于雄蛹,而雄蛹中胸背板最宽,其后缘明显向外凸起.因此,春尺蠖蛹胸部或腹部特征可用于快速、准确地鉴别雌雄性别. 相似文献