全文获取类型
收费全文 | 288篇 |
免费 | 14篇 |
出版年
2023年 | 1篇 |
2022年 | 1篇 |
2021年 | 9篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 12篇 |
2015年 | 12篇 |
2014年 | 21篇 |
2013年 | 20篇 |
2012年 | 28篇 |
2011年 | 29篇 |
2010年 | 18篇 |
2009年 | 10篇 |
2008年 | 18篇 |
2007年 | 21篇 |
2006年 | 12篇 |
2005年 | 9篇 |
2004年 | 13篇 |
2003年 | 10篇 |
2002年 | 9篇 |
2001年 | 7篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 1篇 |
1996年 | 2篇 |
1994年 | 2篇 |
1991年 | 3篇 |
1984年 | 2篇 |
1971年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有302条查询结果,搜索用时 721 毫秒
41.
42.
Daniel R. Anderson Michael J. Duryee Scott W. Shurmur John Y. Um Walter D. Bussey Carlos D. Hunter Robert P. Garvin Harlan R. Sayles Ted R. Mikuls Lynell W. Klassen Geoffrey M. Thiele 《PloS one》2014,9(9)
Malondialdehyde-acetaldehyde adducts (MAA) have been implicated in atherosclerosis. The purpose of this study was to investigate the role of MAA in atherosclerotic disease. Serum samples from controls (n = 82) and patients with; non-obstructive coronary artery disease (CAD), (n = 40), acute myocardial infarction (AMI) (n = 42), or coronary artery bypass graft (CABG) surgery due to obstructive multi-vessel CAD (n = 72), were collected and tested for antibody isotypes to MAA-modifed human serum albumin (MAA-HSA). CAD patients had elevated relative levels of IgG and IgA anti-MAA, compared to control patients (p<0.001). AMI patients had a significantly increased relative levels of circulating IgG anti-MAA-HSA antibodies as compared to stable angina (p<0.03) or CABG patients (p<0.003). CABG patients had significantly increased relative levels of circulating IgA anti-MAA-HSA antibodies as compared to non-obstructive CAD (p<0.001) and AMI patients (p<0.001). Additionally, MAA-modified proteins were detected in the tissue of human AMI lesions. In conclusion, the IgM, IgG and IgA anti-MAA-HSA antibody isotypes are differentially and significantly associated with non-obstructive CAD, AMI, or obstructive multi-vessel CAD and may serve as biomarkers of atherosclerotic disease. 相似文献
43.
Martin W. Bratschi Marie-Thérèse Ruf Arianna Andreoli Jacques C. Minyem Sarah Kerber Fidèle G. Wantong James Pritchard Victoria Chakwera Christian Beuret Matthias Wittwer Djeunga Noumen Nadia Schürch Alphonse Um Book Gerd Pluschke 《PLoS neglected tropical diseases》2014,8(3)
Buruli ulcer (BU), a neglected tropical disease of the skin and subcutaneous tissue, is caused by Mycobacterium ulcerans and is the third most common mycobacterial disease after tuberculosis and leprosy. While there is a strong association of the occurrence of the disease with stagnant or slow flowing water bodies, the exact mode of transmission of BU is not clear. M. ulcerans has emerged from the environmental fish pathogen M. marinum by acquisition of a virulence plasmid encoding the enzymes required for the production of the cytotoxic macrolide toxin mycolactone, which is a key factor in the pathogenesis of BU. Comparative genomic studies have further shown extensive pseudogene formation and downsizing of the M. ulcerans genome, indicative for an adaptation to a more stable ecological niche. This has raised the question whether this pathogen is still present in water-associated environmental reservoirs. Here we show persistence of M. ulcerans specific DNA sequences over a period of more than two years at a water contact location of BU patients in an endemic village of Cameroon. At defined positions in a shallow water hole used by the villagers for washing and bathing, detritus remained consistently positive for M. ulcerans DNA. The observed mean real-time PCR Ct difference of 1.45 between the insertion sequences IS2606 and IS2404 indicated that lineage 3 M. ulcerans, which cause human disease, persisted in this environment after successful treatment of all local patients. Underwater decaying organic matter may therefore represent a reservoir of M. ulcerans for direct infection of skin lesions or vector-associated transmission. 相似文献
44.
S. Ok Y. S. Chung B. Y. Um M. S. Park J.-M. Bae S. J. Lee J. S. Shin 《Plant cell reports》2000,19(9):932-937
A cDNA library was constructed using mRNA prepared from leaves of watermelon [Citrullus lanatus (Thunb.) Matsum&Nakai] at the vegetative stage. Randomly selected cDNA clones were sequenced in order to identify potentially
informative genes. Database comparisons indicated that out of the 704 watermelon cDNA clones, 399 clones (56.7 %) revealed
a high degree of sequence similarity to genes from other organisms. These expressed sequence tag clones were divided into
ten categories depending upon gene function. Since this kind of experiment has not previously been carried out in this genome,
random nucleotide sequencing of these cDNAs could contribute considerable information concerning the novel genes in this organism.
Received: 10 July 1999 / Revision received: 20 December 1999 / Accepted: 11 January 2000 相似文献
45.
Baltimore Harbor (Baltimore, MD) sediments were utilized to initiate anaerobic enrichment cultures with polycyclic aromatic
hydrocarbons (PAHs) in the absence of supplementary electron acceptors. Cultures amended with naphthalene and phenanthrene
exhibited sustained, transferable degradation of the PAHs. Bromoethanesulfonic acid, a selective inhibitor of methanogenesis,
inhibited the degradation of 200 μm naphthalene and phenanthrene; molecular characterization based on 16S rRNA sequences confirmed that methanogenic Archaea were eliminated, thus providing evidence that methanogenesis is involved in the degradation pathway.
Revisions requested 16 November 2005; Revisions received 14 December 2005 相似文献
46.
47.
48.
49.
50.