全文获取类型
收费全文 | 347篇 |
免费 | 40篇 |
专业分类
387篇 |
出版年
2022年 | 3篇 |
2020年 | 3篇 |
2019年 | 7篇 |
2018年 | 9篇 |
2017年 | 3篇 |
2016年 | 7篇 |
2015年 | 6篇 |
2014年 | 9篇 |
2013年 | 19篇 |
2012年 | 20篇 |
2011年 | 12篇 |
2010年 | 15篇 |
2009年 | 14篇 |
2008年 | 15篇 |
2007年 | 14篇 |
2006年 | 20篇 |
2005年 | 13篇 |
2004年 | 15篇 |
2003年 | 8篇 |
2002年 | 4篇 |
2001年 | 12篇 |
2000年 | 5篇 |
1999年 | 6篇 |
1998年 | 7篇 |
1995年 | 6篇 |
1994年 | 2篇 |
1993年 | 5篇 |
1992年 | 7篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 7篇 |
1988年 | 7篇 |
1987年 | 8篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1983年 | 6篇 |
1982年 | 7篇 |
1981年 | 5篇 |
1979年 | 6篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1975年 | 3篇 |
1973年 | 2篇 |
1972年 | 5篇 |
1971年 | 5篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1967年 | 4篇 |
1960年 | 2篇 |
排序方式: 共有387条查询结果,搜索用时 0 毫秒
121.
122.
Metal activation of histidine ammonia-lyase. Metal ion-sulfhydryl group relationship 总被引:2,自引:0,他引:2
C B Klee 《The Journal of biological chemistry》1972,247(5):1398-1406
123.
124.
125.
126.
The Ca2+-dependent regulator protein of cyclic nucleotide phosphodiesterase was labeled with 125I to the extent of 1 mol of monoiodotyrosine per mol. The iodinated protein showed a small decrease in affinity for phosphodiesterase but gave the same maximal level of activation of the enzyme as did the unmodified regulator protein. Iodinated regulator protein formed complexes with both highly purified cyclic nucleotide phosphodiesterase and phosphodiesterase inhibitory protein in the presence but not in the absence of Ca2+ as demonstrated by ultracentrifugation in glycerol gradients. Cross-linking experiments indicate that the Ca2+-dependent regulator protein interacts with the large subunit of the inhibitory protein. 相似文献
127.
128.
129.
Fernanda Abreu Viviana Morillo Fabrícia F Nascimento Clarissa Werneck Mauricio Egidio Cant?o Luciane Prioli Ciapina Luiz Gonzaga Paula de Almeida Christopher T Lefèvre Dennis A Bazylinski Ana Tereza Ribeiro de Vasconcelos Ulysses Lins 《The ISME journal》2014,8(5):1055-1068
Candidatus Magnetoglobus multicellularis (Ca. M. multicellularis) is a member of a group of uncultured magnetotactic prokaryotes that possesses a unique multicellular morphology. To better understand this organism''s physiology, we used a genomic approach through pyrosequencing. Genomic data analysis corroborates previous structural studies and reveals the proteins that are likely involved in multicellular morphogenesis of this microorganism. Interestingly, some detected protein sequences that might be involved in cell adhesion are homologues to phylogenetically unrelated filamentous multicellular bacteria proteins, suggesting their contribution in the early development of multicellular organization in Bacteria. Genes related to the behavior of Ca. M. multicellularis (chemo-, photo- and magnetotaxis) and its metabolic capabilities were analyzed. On the basis of the genomic–physiologic information, enrichment media were tested. One medium supported chemoorganoheterotrophic growth of Ca. M. multicellularis and allowed the microorganisms to maintain their multicellular morphology and cell cycle, confirming for the first time that the entire life cycle of the MMP occurs in a multicellular form. Because Ca. M. multicellularis has a unique multicellular life style, its cultivation is an important achievement for further studies regarding the multicellular evolution in prokaryotes. 相似文献
130.
Sean M. Sliman Rishi B. Patel Jason P. Cruff Sainath R. Kotha Christie A. Newland Carrie A. Schrader Shariq I. Sherwani Travis O. Gurney Ulysses J. Magalang Narasimham L. Parinandi 《Cell biochemistry and biophysics》2013,67(2):399-414
Adiponectin (Ad), an adipokine exclusively secreted by the adipose tissue, has emerged as a paracrine metabolic regulator as well as a protectant against oxidative stress. Pharmacological approaches of protecting against clinical hyperoxic lung injury during oxygen therapy/treatment are limited. We have previously reported that Ad inhibits the NADPH oxidase-catalyzed formation of superoxide from molecular oxygen in human neutrophils. Based on this premise, we conducted studies to determine whether (i) exogenous Ad would protect against the hyperoxia-induced barrier dysfunction in the lung endothelial cells (ECs) in vitro, and (ii) endogenously synthesized Ad would protect against hyperoxic lung injury in wild-type (WT) and Ad-overexpressing transgenic (AdTg) mice in vivo. The results demonstrated that exogenous Ad protected against the hyperoxia-induced oxidative stress, loss of glutathione (GSH), cytoskeletal reorganization, barrier dysfunction, and leak in the lung ECs in vitro. Furthermore, the hyperoxia-induced lung injury, vascular leak, and lipid peroxidation were significantly attenuated in AdTg mice in vivo. Also, AdTg mice exhibited elevated levels of total thiols and GSH in the lungs as compared with WT mice. For the first time, our studies demonstrated that Ad protected against the hyperoxia-induced lung damage apparently through attenuation of oxidative stress and modulation of thiol-redox status. 相似文献