全文获取类型
收费全文 | 2558篇 |
免费 | 220篇 |
专业分类
2778篇 |
出版年
2023年 | 4篇 |
2022年 | 19篇 |
2021年 | 51篇 |
2020年 | 18篇 |
2019年 | 20篇 |
2018年 | 33篇 |
2017年 | 35篇 |
2016年 | 77篇 |
2015年 | 121篇 |
2014年 | 134篇 |
2013年 | 148篇 |
2012年 | 220篇 |
2011年 | 208篇 |
2010年 | 153篇 |
2009年 | 132篇 |
2008年 | 148篇 |
2007年 | 160篇 |
2006年 | 138篇 |
2005年 | 128篇 |
2004年 | 151篇 |
2003年 | 127篇 |
2002年 | 130篇 |
2001年 | 35篇 |
2000年 | 29篇 |
1999年 | 34篇 |
1998年 | 41篇 |
1997年 | 25篇 |
1996年 | 25篇 |
1995年 | 23篇 |
1994年 | 21篇 |
1993年 | 22篇 |
1992年 | 23篇 |
1991年 | 14篇 |
1990年 | 14篇 |
1989年 | 20篇 |
1988年 | 9篇 |
1987年 | 11篇 |
1986年 | 12篇 |
1985年 | 4篇 |
1984年 | 9篇 |
1983年 | 4篇 |
1982年 | 8篇 |
1981年 | 6篇 |
1980年 | 4篇 |
1979年 | 5篇 |
1978年 | 6篇 |
1977年 | 4篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 4篇 |
排序方式: 共有2778条查询结果,搜索用时 15 毫秒
81.
Ralf Bialek Gloria M. González Dominik Begerow Ulrike E. Zelck 《FEMS immunology and medical microbiology》2013,1830(3):355-360
Clinical isolates of Coccidioides spp. and Blastomyces dermatitidis can be identified by chemiluminescent DNA probes and PCR assays targeting multicopy genes. In fixed tissue samples, cells of the two fungi are specified by in situ hybridization and PCR assays targeting 18S rDNA but sequencing of the products is mandatory. Nested PCR assays targeting genes encoding species- or genus-specific proteins like proline rich antigen of Coccidioides spp. and B. dermatitidis adhesin facilitate amplification of specific DNA from fixed tissue samples. The value of DNA amplification from native specimens of suspected cases of coccidioidomycosis or blastomycosis still needs to be determined. 相似文献
82.
83.
There is extensive evidence for an association between an attentional bias towards emotionally negative stimuli and vulnerability to stress-related psychopathology. Less is known about whether selective attention towards emotionally positive stimuli relates to mental health and stress resilience. The current study used a modified Dot Probe task to investigate if individual differences in attentional biases towards either happy or angry emotional stimuli, or an interaction between these biases, are related to self-reported trait stress resilience. In a nonclinical sample (N = 43), we indexed attentional biases as individual differences in reaction time for stimuli preceded by either happy or angry (compared to neutral) face stimuli. Participants with greater attentional bias towards happy faces (but not angry faces) reported higher trait resilience. However, an attentional bias towards angry stimuli moderated this effect: The attentional bias towards happy faces was only predictive for resilience in those individuals who also endorsed an attentional bias towards angry stimuli. An attentional bias towards positive emotional stimuli may thus be a protective factor contributing to stress resilience, specifically in those individuals who also endorse an attentional bias towards negative emotional stimuli. Our findings therefore suggest a novel target for prevention and treatment interventions addressing stress-related psychopathology. 相似文献
84.
Constance Holman Sophie K. Piper Ulrike Grittner Andreas Antonios Diamantaras Jonathan Kimmelman Bob Siegerink Ulrich Dirnagl 《PLoS biology》2016,14(1)
Given small sample sizes, loss of animals in preclinical experiments can dramatically alter results. However, effects of attrition on distortion of results are unknown. We used a simulation study to analyze the effects of random and biased attrition. As expected, random loss of samples decreased statistical power, but biased removal, including that of outliers, dramatically increased probability of false positive results. Next, we performed a meta-analysis of animal reporting and attrition in stroke and cancer. Most papers did not adequately report attrition, and extrapolating from the results of the simulation data, we suggest that their effect sizes were likely overestimated.
Where have all the rodents gone?Ooh ooh, ooh ooh, oohTo non-random attrition, every oneWhen will they ever learn?—with apologies to Pete Seeger, 1955相似文献
85.
86.
Georg Bier Malte N. Bongers Hendrik Ditt Benjamin Bender Ulrike Ernemann Marius Horger 《PloS one》2016,11(1)
Purpose
Ischemic brain edema is subtle and hard to detect by computed tomography within the first hours of stroke onset. We hypothesize that non-enhanced CT (NECT) post-processing with frequency-selective non-linear blending (“best contrast”/BC) increases its accuracy in detecting edema and irreversible tissue damage (infarction).Methods
We retrospectively analyzed the NECT scans of 76 consecutive patients with ischemic stroke (exclusively middle cerebral artery territory—MCA) before and after post-processing with BC both at baseline before reperfusion therapy and at follow-up (5.73±12.74 days after stroke onset) using the Alberta Stroke Program Early CT Score (ASPECTS). We assessed the differences in ASPECTS between unprocessed and post-processed images and calculated sensitivity, specificity, and predictive values of baseline NECT using follow-up CT serving as reference standard for brain infarction.Results
NECT detected brain tissue hypoattenuation in 35 of 76 patients (46.1%). This number increased to 71 patients (93.4%) after post-processing with BC. Follow-up NECT confirmed brain infarctions in 65 patients (85.5%; p = 0.012). Post-processing increased the sensitivity of NECT for brain infarction from 35/65 (54%) to 65/65 (100%), decreased its specificity from 11/11 (100%) to 7/11 (64%), its positive predictive value (PPV) from 35/35 (100%) to 65/69 (94%) and increased its accuracy 46/76 (61%) to 72/76 (95%).Conclusions
This post-hoc analysis suggests that post-processing of NECT with BC may increase its sensitivity for ischemic brain damage significantly. 相似文献87.
Alba?SulajEmail author Johanna?Zemva Ulrike?Zech Annika?Woehning Maik?Brune Gottfried?Rudofsky Peter?P.?Nawroth Thomas?Fleming Rüdiger?von Bauer 《BMC endocrine disorders》2016,16(1):56
Background
The aim of this study was to investigate the effect of a lifestyle intervention in obesity on the soluble form of the activated leukocyte cell adhesion molecule (sALCAM) and its association with metabolic parameters.Methods
Twenty-nine obese subjects selected from the OPTIFAST®52 program. This program consisted into 2 crucial phases: an initial 12-week active weight reduction phase, followed by a 40-week weight maintenance phase. At baseline, after 12 weeks and at the end of the program, fasting glucose and insulin, total cholesterol, LDL-C, HDL-C, triglycerides, adiponectin, leptin, high sensitivity CRP, sALCAM, homeostasis model assessment-estimated insulin resistance (HOMA-IR) and leptin-to-adiponectin-ratio were determined. Oral glucose tolerance test (OGTT) was performed when indicated.Results
At baseline, the serum concentration of sALCAM was increased and correlated positively with HOMA-IR and negatively with age. At the end of the program, sALCAM concentrations decreased significantly. Multivariate analysis showed that sALCAM significantly correlated with age, glucose concentration after 2 h OGTT and the HOMA-IR. A higher decrease of HOMA-IR during the study was observed in subjects with higher concentration of sALCAM at baseline.Conclusions
sALCAM might be a novel biomarker in obesity that correlates and predicts insulin sensitivity improvement and that can be affected by lifestyle intervention.88.
89.
RNAi experiments are ubiquitously used in cell biology and are achieved by transfection of small interfering RNAs (siRNAs) into cells using a transfection reagent. These results in knock‐down of proteins of interest, and the phenotypic consequences are then analyzed. It is reported here that two common RNA interference (RNAi) transfection reagents, DharmaFECT 1 and INTERFERin, in mock transfections using non‐targeting siRNAs, cause alterations in the lipidome of HeLa cells. Some lipids change in response to both, presumably chemically different, transfection reagents, while other lipid species change only in response to one of the reagents. While the functional implications of these lipidomic alterations remain to be investigated, the authors' experiments suggest that it is important to use appropriate mock transfection controls during RNAi experiments, ideally complemented by an orthogonal perturbation, especially when investigating membrane‐associated phenomena. 相似文献
90.