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61.
Tree resistance to Lymantria dispar caterpillars: importance and limitations of foliar tannin composition 总被引:1,自引:0,他引:1
Raymond V. Barbehenn Adam Jaros Grace Lee Cara Mozola Quentin Weir Juha-Pekka Salminen 《Oecologia》2009,159(4):777-788
The ability of foliar tannins to increase plant resistance to herbivores is potentially determined by the composition of the
tannins; hydrolyzable tannins are much more active as prooxidants in the guts of caterpillars than are condensed tannins.
By manipulating the tannin compositions of two contrasting tree species, this work examined: (1) whether increased levels
of hydrolyzable tannins increase the resistance of red oak (Quercus rubra L.), a tree with low resistance that produces mainly condensed tannins, and (2) whether increased levels of condensed tannins
decrease the resistance of sugar maple (Acer saccharum Marsh.), a tree with relatively high resistance that produces high levels of hydrolyzable tannins. As expected, when Lymantria dispar L. caterpillars ingested oak leaves coated with hydrolyzable tannins, levels of hydrolyzable tannin oxidation increased in
their midgut contents. However, increased tannin oxidation had no significant impact on oxidative stress in the surrounding
midgut tissues. Although growth efficiencies were decreased by hydrolyzable tannins, growth rates remained unchanged, suggesting
that additional hydrolyzable tannins are not sufficient to increase the resistance of oak. In larvae on condensed tannin-coated
maple, no antioxidant effects were observed in the midgut, and levels of tannin oxidation remained high. Consequently, neither
oxidative stress in midgut tissues nor larval performance were significantly affected by high levels of condensed tannins.
Post hoc comparisons of physiological mechanisms related to tree resistance revealed that maple produced not only higher levels
of oxidative stress in the midgut lumen and midgut tissues of L. dispar, but also decreased protein utilization efficiency compared with oak. Our results suggest that high levels of hydrolyzable
tannins are important for producing oxidative stress, but increased tree resistance to caterpillars may require additional
factors, such as those that produce nutritional stress. 相似文献
62.
The extent to which individual host trees maintain their relative quality over time may affect patterns of abundance, distribution and microevolution in herbivorous insects. In this study, we explore temporal consistency in the quality of oak Quercus robur foliage, using leaf‐chewing larvae of the moth Amphipyra pyramidea as our model herbivores. By utilising an artificial diet, we are able to isolate the impact of chemical contents from physical attributes, and thereby to ask to what extent purely chemical parameters create tree‐to‐tree differences in host quality, how consistent such differences are among trees between different parts of a single growth season, and to what extent individual moth larvae are able to compensate for chemical variation in food quality. We find that with physical traits controlled for, chemical traits suffice to create strong differences in larval growth rates between trees, and between larvae fed on young and mature foliage. Nevertheless, these initial differences are efficiently compensated for the fact that larvae with lower growth rates continue to grow for a longer time, and thereby end up at the same size as larvae with high growth rates. At the pupal stage, we could no longer detect differences between either larvae fed foliage from different trees or between larvae fed young versus mature foliage – despite notably little variation among individuals within each group. Such compensatory responses were also reflected in patterns of consistency. The intraclass correlation for larval weight was relatively high (ρ=0.45), but lower for development time (ρ=0.26), and non‐existent for pupal weight (ρ=0.00). These results suggest that in terms of pupal mass, A. pyramidea is able to compensate more or less completely for differences in resource quality, that patterns of consistency vary with the specific trait examined, and that the net effect of spatiotemporal variation in host plant quality on herbivore fitness should be dissected by experiments aimed at different life history traits. If slow growth comes with high mortality, spatiotemporal patterns in resource quality may have a major impact on herbivore fitness; if not, the patterns may be nullified by efficient compensatory mechanisms. 相似文献
63.
The relationship among temporal variation in the availability of carotenoid-rich food, tissue carotenoid levels and breeding success are poorly known. We studied how diet quality and quantity affect the carotenoid profile and fledging success of great tit (Parus major) nestlings along a pollution gradient. We found declining seasonal trend in lutein concentration of caterpillars, which may be the explanation for the declining trend in nestlings' lutein concentration of plasma with season, despite the increase in caterpillar biomass. This may be because the biomass of most lutein-rich caterpillars (autumnal moths) decreased and less lutein-rich caterpillars (sawflies) increased during the breeding season. The temporal difference in occurrence of different caterpillar species means that peak lutein availability does not coincide with peak caterpillar abundance. However the positive association between total larval biomass and the number of great tit fledglings may suggest that fledging success depends more on total caterpillar availability than on lutein concentration of caterpillars. 相似文献
64.
Background
Previous work on the human auditory cortex has revealed areas specialized in spatial processing but how the neurons in these areas represent the location of a sound source remains unknown.Methodology/Principal Findings
Here, we performed a magnetoencephalography (MEG) experiment with the aim of revealing the neural code of auditory space implemented by the human cortex. In a stimulus-specific adaptation paradigm, realistic spatial sound stimuli were presented in pairs of adaptor and probe locations. We found that the attenuation of the N1m response depended strongly on the spatial arrangement of the two sound sources. These location-specific effects showed that sounds originating from locations within the same hemifield activated the same neuronal population regardless of the spatial separation between the sound sources. In contrast, sounds originating from opposite hemifields activated separate groups of neurons.Conclusions/Significance
These results are highly consistent with a rate code of spatial location formed by two opponent populations, one tuned to locations in the left and the other to those in the right. This indicates that the neuronal code of sound source location implemented by the human auditory cortex is similar to that previously found in other primates. 相似文献65.
Tapio Eeva Suvi Ruuskanen Juha-Pekka Salminen Eugen Belskii Antero J?rvinen Anvar Kerimov Erkki Korpim?ki Indrikis Krams Juan Moreno Chiara Morosinotto Raivo M?nd Markku Orell Anna Qvarnstr?m Heli Siitari Fred M. Slater Vallo Tilgar Marcel E. Visser Wolfgang Winkel Herwig Zang Toni Laaksonen 《Oecologia》2011,165(2):277-287
Carotenoids in the egg yolks of birds are considered to be important antioxidants and immune stimulants during the rapid growth of embryos. Yolk carotenoid composition is strongly affected by the carotenoid composition of the female??s diet at the time of egg formation. Spatial and temporal differences in carotenoid availability may thus be reflected in yolk concentrations. To assess whether yolk carotenoid concentrations or carotenoid profiles show any large-scale geographical trends or differences among habitats, we collected yolk samples from 16 European populations of the pied flycatcher, Ficedula hypoleuca. We found that the concentrations and proportions of lutein and some other xanthophylls in the egg yolks decreased from Central Europe northwards. The most southern population (which is also the one found at the highest altitude) also showed relatively low carotenoid levels. Concentrations of ??-carotene and zeaxanthin did not show any obvious geographical gradients. Egg yolks also contained proportionally more lutein and other xanthophylls in deciduous than in mixed or coniferous habitats. We suggest that latitudinal gradients in lutein and xanthophylls reflect the lower availability of lutein-rich food items in the northern F. hypoleuca populations and in montane southern populations, which start egg-laying earlier relative to tree phenology than the Central European populations. Similarly, among-habitat variation is likely to reflect the better availability of lutein-rich food in deciduous forests. Our study is the first to indicate that the concentration and profile of yolk carotenoids may show large-scale spatial variation among populations in different parts of the species?? geographical range. Further studies are needed to test the fitness effects of this geographical variation. 相似文献
66.
Jetro J. Tuulari Henry K. Karlsson Jussi Hirvonen Paulina Salminen Pirjo Nuutila Lauri Nummenmaa 《PloS one》2015,10(2)
The mere sight of foods may activate the brain’s reward circuitry, and humans often experience difficulties in inhibiting urges to eat upon encountering visual food signals. Imbalance between the reward circuit and those supporting inhibitory control may underlie obesity, yet brain circuits supporting volitional control of appetite and their possible dysfunction that can lead to obesity remain poorly specified. Here we delineated the brain basis of volitional appetite control in healthy and obese individuals with functional magnetic resonance imaging (fMRI). Twenty-seven morbidly obese women (mean BMI = 41.4) and fourteen age-matched normal-weight women (mean BMI = 22.6) were scanned with 1.5 Tesla fMRI while viewing food pictures. They were instructed to inhibit their urge to eat the foods, view the stimuli passively or imagine eating the foods. Across all subjects, a frontal cortical control circuit was activated during appetite inhibition versus passive viewing of the foods. Inhibition minus imagined eating (appetite control) activated bilateral precunei and parietal cortices and frontal regions spanning anterior cingulate and superior medial frontal cortices. During appetite control, obese subjects had lower responses in the medial frontal, middle cingulate and dorsal caudate nuclei. Functional connectivity of the control circuit was increased in morbidly obese versus control subjects during appetite control, which might reflect impaired integrative and executive function in obesity. 相似文献
67.
68.
Functional characterization of Gne (UDP-N-acetylglucosamine-4-epimerase), Wzz (chain length determinant), and Wzy (O-antigen polymerase) of Yersinia enterocolitica serotype O:8 总被引:4,自引:0,他引:4 下载免费PDF全文
Bengoechea JA Pinta E Salminen T Oertelt C Holst O Radziejewska-Lebrecht J Piotrowska-Seget Z Venho R Skurnik M 《Journal of bacteriology》2002,184(15):4277-4287
The lipopolysaccharide (LPS) O-antigen of Yersinia enterocolitica serotype O:8 is formed by branched pentasaccharide repeat units that contain N-acetylgalactosamine (GalNAc), L-fucose (Fuc), D-galactose (Gal), D-mannose (Man), and 6-deoxy-D-gulose (6d-Gul). Its biosynthesis requires at least enzymes for the synthesis of each nucleoside diphosphate-activated sugar precursor; five glycosyltransferases, one for each sugar residue; a flippase (Wzx); and an O-antigen polymerase (Wzy). As this LPS shows a characteristic preferred O-antigen chain length, the presence of a chain length determinant protein (Wzz) is also expected. By targeted mutagenesis, we identify within the O-antigen gene cluster the genes encoding Wzy and Wzz. We also present genetic and biochemical evidence showing that the gene previously called galE encodes a UDP-N-acetylglucosamine-4-epimerase (EC 5.1.3.7) required for the biosynthesis of the first sugar of the O-unit. Accordingly, the gene was renamed gne. Gne also has some UDP-glucose-4-epimerase (EC 5.1.3.2) activity, as it restores the core production of an Escherichia coli K-12 galE mutant. The three-dimensional structure of Gne was modeled based on the crystal structure of E. coli GalE. Detailed structural comparison of the active sites of Gne and GalE revealed that additional space is required to accommodate the N-acetyl group in Gne and that this space is occupied by two Tyr residues in GalE whereas the corresponding residues present in Gne are Leu136 and Cys297. The Gne Leu136Tyr and Cys297Tyr variants completely lost the UDP-N-acetylglucosamine-4-epimerase activity while retaining the ability to complement the LPS phenotype of the E. coli galE mutant. Finally, we report that Yersinia Wzx has relaxed specificity for the translocated oligosaccharide, contrary to Wzy, which is strictly specific for the O-unit to be polymerized. 相似文献
69.
Nuutinen T Huuskonen J Suuronen T Ojala J Miettinen R Salminen A 《Neurochemistry international》2007,50(3):540-547
Recent studies indicate that astrocytes may be the primary target of secreted amyloid-beta 1-42 peptides, with the neurotoxicity representing a secondary response to astrocytic stress. Our purpose was to clarify the astrocytic stress response induced by amyloid-beta peptides in human and rat astrocytes. Human amyloid-beta 1-42 peptides and fibrils induced the appearance of cytoplasmic vacuoles in normal human astrocytes (NHA) and CCFsttg1 astrocytoma cells. Vacuoles appeared 9-12h after the amyloid-beta exposure and remained present for several days. Rat primary neonatal astrocytes showed similar but less prominent vacuolar response. Human amyloid-beta peptides 1-16, 1-28, 10-20, 17-21 and 25-35 did not cause vacuole formation. Electron microscopic observations revealed large endocytic vacuoles containing fibrillar amyloid material. Stress marker analysis did not show any increase in protein levels of HSP70, HSP90, GRP78 and GRP94. However, the protein level of clusterin/apoJ, a secreted chaperone, was strongly increased both in NHA and CCFsttg1 astrocytes. Endocytic response associated with the accumulation of clusterin/apoJ protein suggests that clusterin/apoJ has a role in the clearance of amyloid-beta peptides. 相似文献
70.
Age- and sex-related differences in lipid peroxidation of mouse cardiac and skeletal muscles 总被引:1,自引:0,他引:1
A Salminen P Saari M Kihlstr?m 《Comparative biochemistry and physiology. B, Comparative biochemistry》1988,89(4):695-699
1. The purpose of the present study was to characterize age- and sex-related changes in lipid peroxidation capacities and enzymatic antioxidants of cardiac and skeletal muscles in NMRI-mice (Mus musculus). 2. Lipid peroxidation rates (unstimulated and enzymatic/iron-stimulated) strongly decreased in skeletal muscle during ageing. 3. Unstimulated lipid peroxidation rate but not that of stimulated, also decreased in cardiac muscle. 4. The total level of Fe2+/ascorbate-stimulated non-enzymatic lipid peroxidation was not, however, affected by ageing. 5. The activity of catalase slightly increased in cardiac muscle and that of glutathione peroxidase in skeletal muscle during ageing. 6. Unstimulated lipid peroxidation rate was significantly higher in the skeletal muscle of male than female mice. 7. Correspondingly, the Fe2+/ascorbate-stimulated lipid peroxidation capacities of microsomal and mitochondrial fractions of skeletal muscle were significantly higher in male mice. 8. The activity of glutathione peroxidase as well as the concentration of lipofuscin were higher in the cardiac muscles of female than male mice. 相似文献