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921.
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U. Henning und C. Herz 《Molecular & general genetics : MGG》1964,95(3):260-275
Summary Acetateless mutants ofEscherichia coli K 12 lacking the enzymatic activities either of the carboxylase, the lipoic reductase-transacetylase or of all components of the pyruvate dehydrogenase complex are shown to be the consequence of mutations in the closely linked structural genes for the constituent enzymes of the enzyme complex. This genetic segment (the acetate locus = Ac) was found on theE. coli chromosome between the leucine and TR loci. Acetate and leucine loci are transduced jointly by the phage Plkc. The preparation is described of double mutants carrying two genetic lesions in the pyruvate dehydrogenase structural gene cluster. The mutant sites of 00-type strains have been localized in a part of the carboxylase structural gene corresponding to the left extremity (nearest the leucine locus) of the acetate locus.Studies on possible genetic relationships between the pyruvate and -ketoglutarate dehydrogenases (which regarding the individual reactions catalyzed are very similar and partly identical) revealed that the two -keto acid dehydrogenases most likely do not share any genetic determinant.
Mit 5 Textabbildungen 相似文献
Mit 5 Textabbildungen 相似文献
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929.
Effect of Ethanol on the Sodium and Potassium Conductances of the Squid Axon Membrane 总被引:8,自引:6,他引:2
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The effects of ethanol on squid giant axons were studied by means of the sucrose-gap technique. The membrane action potential height is moderately reduced and the duration sometimes shortened by ethanol in sea water. Voltage clamp experiments showed that ethanol in sea water reduced the maximum membrane conductances for sodium (g'Na) and potassium (g'K). In experiments with multiple application of ethyl alcohol to the same spot of membrane, a reduction of g'Na to 82 per cent and of g'K to 80 per cent of their value in sea water was brought about by 3 per cent ethanol (by volume) while 6 per cent caused a decrease of g'Na to 59 per cent and of g'K to 69 per cent. Ethanol has no significant effect on the steady-state inactivation of gNa (as a function of conditioning membrane potential) or on such kinetic parameters as τh or the time course of turning on gi gNa and gK. It is concluded that ethanol mainly reduces gNa and gK in the Hodgkin-Huxley terminology. 相似文献
930.
Werner Ulbricht 《The Journal of general physiology》1964,48(1):113-127
Action potentials of single frog nerve fibers were recorded with the air-gap method in "low Ca" (0.26 mM) and "high Ca" (4.2 mM) solutions and compared to spikes in normal Ringer''s (1.05 mM Ca). On increasing (Ca)o the action potentials became shorter, the "knee" during the falling phase as well as the threshold for abolition moved to internal potentials more positive, and the spike recovery during the relative refractory period was faster. Outward current pulses applied during an action potential affected its configuration more in low Ca than in high Ca. The onset of the delayed rectification (in the absence of Na) was found faster in high Ga. After-potentials during anelectrotonus declined more rapidly in high Ca than in low Ca. The results are compared primarily with the voltage-clamp analysis of Ca effects on squid axons and satisfactory qualitative agreement is reached. 相似文献