MORPHOLOGICAL AND BIOCHEMICAL CORRELATES OF CEREBRAL MICROSOMES : I. Isolation and Chemical Characterization

Microsomal fractions, both homogeneous in appearance and functionally operative, were isolated from a homogenate of rat cerebral cortex by fractionation in water. The preparations thus obtained contain the membranous elements of the endoplasmic reticulum, synaptic vesicles, and ribosomes. Esterase, ATPase, and glutamine synthetase were found to be present and fully functional in the microsomal fractions isolated in water. The contamination of the water-isolated microsomal fractions by mitochondria and lysosomes was found to be considerably lower than in microsomal pellets isolated in sucrose. The contamination by nerve ending particles, as judged by electron microscopy and by the levels of soluble lactic dehydrogenase entrapped in the cytoplasm of the particles, was also low. Most of the contamination by mitochondria and nerve ending particles could be removed by treatment of the microsomal pellet with 150 mM NaCl. Resistant to elution by this treatment is the lysosomal contamination as well as microsomal esterase and ATPase. Glutamine synthetase, on the other hand, was almost totally solubilized. Microsomal preparations isolated in water are also shown to contain amounts of protein, RNA, phospholipid, and ganglioside comparable to those found in microsomal preparations isolated in sucrose.     

Stimulation of Carotenogenesis by Microbial Cells

Spent mycelia of Blakeslea trispora recovered from a previous fermentation enhanced carotene production by mated cultures of the same organism. Peak yields of 107 to 142 mg of carotene per 100 ml of medium were achieved in 6 days. β-Carotene constituted 92% of the total carotenoids produced. The enhancing substance was found present in an aqueous extract of the mycelium. Part of its activity was due to an organic acid fraction. Other microbial cells, molds, yeasts, and bacteria were also capable of enhancing carotene production.     

131I in the study of the fate of proteins in the organism

Авторы изучили исче зновения от su131 () Я марки ровкой бычьего сыво роточного альбумина из поток крови и ткан ей, и его распределен ие в subcellular печени дроби у кроликов, в ранний п ериод после администрации, в ход е наиболее заметным иммунологической ре акции и после 19 дней. Результаты этих эксп ериментов показыва ют, что в исследовани и судьбы белка антигенов в организ м белков йодированн ой пригодны для перв ого этапа только, i. д. распределение в тка ни, жидкости, белка половину жизни и имм унной фазы исчезнов ения от антигена. Так как в контрольны х группах, к которым K () su131 управление, йода со храняется в тканях деятельность по 19 ден ь, никто не может исключать возможно сть-даже в экспериме нтах в котором йод с рекламой белка была использована, то в настоящее время деятельность ткани в последующих этапов уже не доказ ательства присутст вия белка.