全文获取类型
收费全文 | 91篇 |
免费 | 9篇 |
国内免费 | 1篇 |
专业分类
101篇 |
出版年
2022年 | 1篇 |
2021年 | 2篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 3篇 |
2016年 | 1篇 |
2015年 | 6篇 |
2014年 | 14篇 |
2013年 | 6篇 |
2012年 | 11篇 |
2011年 | 3篇 |
2010年 | 8篇 |
2009年 | 4篇 |
2008年 | 3篇 |
2007年 | 6篇 |
2006年 | 4篇 |
2005年 | 2篇 |
2004年 | 4篇 |
2003年 | 1篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1981年 | 1篇 |
排序方式: 共有101条查询结果,搜索用时 15 毫秒
81.
82.
Bengtsson S Krogh M Szigyarto CA Uhlen M Schedvins K Silfverswärd C Linder S Auer G Alaiya A James P 《Journal of proteome research》2007,6(4):1440-1450
Ovarian cancer is usually found at a late stage when the prognosis is often bad. Relative survival rates decrease with tumor stage or grade, and the 5-year survival rate for women with carcinoma is only 38%. Thus, there is a great need to find biomarkers that can be used to carry out routine screening, especially in high-risk patient groups. Here, we present a large-scale study of 64 tissue samples taken from patients at all stages and show that we can identify statistically valid markers using nonsupervised methods that distinguish between normal, benign, borderline, and malignant tissue. We have identified 217 of the significantly changing protein spots. We are expressing and raising antibodies to 35 of these. Currently, we have validated 5 of these antibodies for use in immunohistochemical analysis using tissue microarrays of healthy and diseased ovarian, as well as other, human tissues. 相似文献
83.
84.
Tim Downing David J. Lynn Sarah Connell Andrew T. Lloyd AK Fazlul Haque Bhuiyan Pradeepa Silva Arifa N. Naqvi Rahamame Sanfo Racine-Samba Sow Baitsi Podisi Cliona O’Farrelly Olivier Hanotte Daniel G. Bradley 《Immunogenetics》2009,61(4):303-314
There have been significant evolutionary pressures on the chicken during both its speciation and its subsequent domestication
by man. Infectious diseases are expected to have exerted strong selective pressures during these processes. Consequently,
it is likely that genes associated with disease susceptibility or resistance have been subject to some form of selection.
Two genes involved in the immune response (interferon-γ and interleukin 1-β) were selected for sequencing in diverse chicken
populations from Pakistan, Sri Lanka, Bangladesh, Kenya, Senegal, Burkina Faso and Botswana, as well as six outgroup samples
(grey, green, red and Ceylon jungle fowl and grey francolin and bamboo partridge). Haplotype frequencies, tests of neutrality,
summary statistics, coalescent simulations and phylogenetic analysis by maximum likelihood were used to determine the population
genetic characteristics of the genes. Networks indicate that these chicken genes are most closely related to the red jungle
fowl. Interferon-γ had lower diversity and considerable coding sequence conservation, which is consistent with its function
as a key inflammatory cytokine of the immune response. In contrast, the pleiotropic cytokine interleukin 1-β had higher diversity
and showed signals of balancing selection moderated by recombination, yielding high numbers of diverse alleles, possibly reflecting
broader functionality and potential roles in more diseases in different environments.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
An erratum to this article can be found at 相似文献
85.
86.
87.
Thomas Hultman Stefan Ståhl Tomas Moks Mathias Uhlen 《Nucleosides, nucleotides & nucleic acids》2013,32(5-6):629-638
Abstract A novel approach to enable two-directional solid-phase DNA sequencing, involving immobilization of double stranded plasmid to avidin agarose, is described. A plasmid vector, pRIT28, has been designed to allow enzymatic incorporation of biotinylated dUTP into two alternative sites. 相似文献
88.
Fagerberg L Strömberg S El-Obeid A Gry M Nilsson K Uhlen M Ponten F Asplund A 《Journal of proteome research》2011,10(9):4066-4075
Human cancer cell lines grown in vitro are frequently used to decipher basic cell biological phenomena and to also specifically study different forms of cancer. Here we present the first large-scale study of protein expression patterns in cell lines using an antibody-based proteomics approach. We analyzed the expression pattern of 5436 proteins in 45 different cell lines using hierarchical clustering, principal component analysis, and two-group comparisons for the identification of differentially expressed proteins. Our results show that immunohistochemically determined protein profiles can categorize cell lines into groups that overall reflect the tumor tissue of origin and that hematological cell lines appear to retain their protein profiles to a higher degree than cell lines established from solid tumors. The two-group comparisons reveal well-characterized proteins as well as previously unstudied proteins that could be of potential interest for further investigations. Moreover, multiple myeloma cells and cells of myeloid origin were found to share a protein profile, relative to the protein profile of lymphoid leukemia and lymphoma cells, possibly reflecting their common dependency of bone marrow microenvironment. This work also provides an extensive list of antibodies, for which high-resolution images as well as validation data are available on the Human Protein Atlas ( www.proteinatlas.org ), that are of potential use in cell line studies. 相似文献
89.
A codon-based model designed to describe lentiviral evolution 总被引:7,自引:6,他引:1
90.