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51.
Polymer-bound nitridomolybdenum(VI) complexes, MoNCl3(polystyrene-bound bipyridyl) (I), MoNCl2(bpy)(polystyrene-bound benzylthiolato) (II), and MoNCl (S-t-Bu)(bpy)(polystyrene-bound benzylthiolato) (III), were synthesized by the reaction of MoNCl3(CH3CN)x or MoNCl3(bpy) with polystyrene-bound bipyridyl or benzylthiol. The polymer-bound nitridomolybdenum complexes were characterized by photo-acoustic and resonance Raman spectra. Hydrolysis or hydrolytic reduction of the nitridomolybdenum(VI) complexes resulted in the formation of ammonia in the following order of yield: III & II & I. Coordination of the polymer thiolato ligands is thus important in enhancing reductive cleavage of the nitridomolybdenum bond. 相似文献
52.
Hearing vulnerability after noise exposure in a mouse model of reactive oxygen species overproduction 下载免费PDF全文
Shigefumi Morioka Hirofumi Sakaguchi Taro Yamaguchi Yuzuru Ninoyu Hiroaki Mohri Takashi Nakamura Yasuo Hisa Kiyokazu Ogita Naoaki Saito Takehiko Ueyama 《Journal of neurochemistry》2018,146(4):459-473
53.
Maria Teresa Borrello Benjamin Schinor Katharina Bartels Hanae Benelkebir Sara Pereira Wafa T. Al-Jamal Leon Douglas Patrick J. Duriez Graham Packham Günter Haufe A. Ganesan 《Bioorganic & medicinal chemistry letters》2017,27(10):2099-2101
We report a series of tranylcypromine analogues containing a fluorine in the cyclopropyl ring. A number of compounds with additional m- or p-substitution of the aryl ring were micromolar inhibitors of the LSD1 enzyme. In cellular assays, the compounds inhibited the proliferation of acute myeloid leukemia cell lines. Increased levels of the biomarkers H3K4me2 and CD86 were consistent with LSD1 target engagement. 相似文献
54.
Yuki Ueyama 《Journal of computational neuroscience》2017,43(1):93-106
Primary motor cortex (M1) neurons are tuned in response to several parameters related to motor control, and it was recently reported that M1 is important in feedback control. However, it remains unclear how M1 neurons encode information to control the musculoskeletal system. In this study, we examined the underlying computational mechanisms of M1 based on optimal feedback control (OFC) theory, which is a plausible hypothesis for neuromotor control. We modelled an isometric torque production task that required joint torque to be regulated and maintained at desired levels in a musculoskeletal system physically constrained by muscles, which act by pulling rather than pushing. Then, a feedback controller was computed using an optimisation approach under the constraint. In the presence of neuromotor noise, known as signal-dependent noise, the sensory feedback gain is tuned to an extrinsic motor output, such as the hand force, like a population response of M1 neurons. Moreover, a distribution of the preferred directions (PDs) of M1 neurons can be predicted via feedback gain. Therefore, we suggest that neural activity in M1 is optimised for the musculoskeletal system. Furthermore, if the feedback controller is represented in M1, OFC can describe multiple representations of M1, including not only the distribution of PDs but also the response of the neuronal population. 相似文献
55.
Functional characterization of the neuron‐restrictive silencer element in the human tryptophan hydroxylase 2 gene expression 下载免费PDF全文
56.
Haruo Hashimoto Tomoo Eto Tsutomu Kamisako Naoko Hoya Teruhiko Hatakeyama Toshiro Arai Makoto Yokosuka Yasuyuki Ohnishi Mamoru Ito Kyoji Hioki Ryo Suzuki Mitsuru Ohsugi Muneo Saito Yoshito Ueyama Toshimasa Yamauchi Naoto Kubota Kazuyuki Tobe Takashi Kadowaki Norikazu Tamaoki Tatsuji Nomura Kinori Kosaka 《Experimental Animals》2008,57(4):407-411
Efficient reproduction using natural mating and reproduction technology [in vitro fertilization (IVF) and embryo transfer (ET)] was investigated in IRS2 deficient mice with C57BL/6JJcl genetic background (Irs2(-/-) mice) as a typical type 2 diabetes model. From the results using various combinations of Irs2(-/-) and Irs2(-/+) mice, the combination of female Irs2(-/+) x male Irs2(-/-) was found to be more efficient than other combinations. In applications of reproduction technology using IVF and ET, the combination of female Irs2(-/+) x male Irs2(-/-) involves the possibility of Irs2(-/-) production by repeats using female Irs2(-/+) mice. However, reproductive continuity using this combination is difficult because of dependence on human technique and the cost of ET. Therefore, we concluded that Irs2(-/-) mice should be produced by embryo transfer using Irs2(-/-) mice from a colony consisting of female Irs2(-/+) x male Irs2(-/-). 相似文献
57.
He J Song Y Ueyama N Saito A Azakami H Kato A 《Protein science : a publication of the Protein Society》2006,15(2):213-222
To address the role of glycosylation on fibrillogenicity of amyloidogenic chicken cystatin, the consensus sequence for N-linked glycosylation (Asn106-Ile108 --> Asn106-Thr108) was introduced by site-directed mutagenesis into the wild-type and amyloidogenic chicken cystatins to construct the glycosylated form of chicken cystatins. Both the glycosylated and unglycosylated forms of wild-type and amyloidogenic mutant I66Q cystatin were expressed and secreted in a culture medium of yeast Pichia pastoris transformants. Comparison of the amount of insoluble aggregate, the secondary structure, and fibrillogenicity has shown that the N-linked glycosylation could prevent amyloid fibril formation of amyloidogenic chicken cystatin secreted in yeast cells without affecting its inhibitory activities. Further study showed this glycosylation could inhibit the formation of cystatin dimers. Therefore, our data strongly suggested that the mechanism causing the prevention of amyloidogenic cystation fibril formation may be realized through suppression of the formation of three-dimensional domain-swapped dimers and oligomers of amyloidogenic cystatin by the glycosylated chains at position 106. 相似文献
58.
Matsubara T Shirai Y Miyasaka K Murakami T Yamaguchi Y Ueyama T Kai M Sakane F Kanoh H Hashimoto T Kamada S Kikkawa U Saito N 《The Journal of biological chemistry》2006,281(10):6152-6164
Diacylglycerol kinases (DGKs) convert diacylglycerol (DG) to phosphatidic acid, and both lipids are known to play important roles in lipid signal transduction. Thereby, DGKs are considered to be a one of the key players in lipid signaling, but its physiological function remains to be solved. In an effort to investigate one of nine subtypes, we found that DGKgamma came to be localized in the nucleus with time in all cell lines tested while seen only in the cytoplasm at the early stage of culture, indicating that DGKgamma is transported from the cytoplasm to the nucleus. The nuclear transportation of DGKgamma didn't necessarily need DGK activity, but its C1 domain was indispensable, suggesting that the C1 domain of DGKgamma acts as a nuclear transport signal. Furthermore, to address the function of DGKgamma in the nucleus, we produced stable cell lines of wild-type DGKgamma and mutants, including kinase negative, and investigated their cell size, growth rate, and cell cycle. The cells expressing the kinase-negative mutant of DGKgamma were larger in size and showed slower growth rate, and the S phase of the cells was extended. These findings implicate that nuclear DGKgamma regulates cell cycle. 相似文献
59.
Onozuka I Kakinuma S Kamiya A Miyoshi M Sakamoto N Kiyohashi K Watanabe T Funaoka Y Ueyama M Nakagawa M Koshikawa N Seiki M Nakauchi H Watanabe M 《Biochemical and biophysical research communications》2011,406(1):134-140
Matrix metalloproteinase (MMP) plays an important role in homeostatic regulation of the extracellular environment and degradation of matrix. During liver fibrosis, several MMPs, including MMP-2, are up-regulated in activated hepatic stellate cells, which are responsible for exacerbation of liver cirrhosis. However, it remains unclear how loss of MMP-2 influences molecular dynamics associated with fibrogenesis in the liver. To explore the role of MMP-2 in hepatic fibrogenesis, we employed two fibrosis models in mice; toxin (carbon tetrachloride, CCl4)-induced and cholestasis-induced fibrosis. In the chronic CCl4 administration model, MMP-2 deficient mice exhibited extensive liver fibrosis as compared with wild-type mice. Several molecules related to activation of hepatic stellate cells were up-regulated in MMP-2 deficient liver, suggesting that myofibroblastic change of hepatic stellate cells was promoted in MMP-2 deficient liver. In the cholestasis model, fibrosis in MMP-2 deficient liver was also accelerated as compared with wild type liver. Production of tissue inhibitor of metalloproteinase 1 increased in MMP-2 deficient liver in both models, while transforming growth factor β, platelet-derived growth factor receptor and MMP-14 were up-regulated only in the CCl4 model. Our study demonstrated, using 2 experimental murine models, that loss of MMP-2 exacerbates liver fibrosis, and suggested that MMP-2 suppresses tissue inhibitor of metalloproteinase 1 up-regulation during liver fibrosis. 相似文献
60.
Binti Md Isa K Kawasaki N Ueyama K Sumii T Kudo S 《Biochemical and biophysical research communications》2011,412(2):318-322
Endothelial nitric oxide synthase (eNOS) is the primary enzyme that produces nitric oxide (NO), which plays an important role in blood vessel relaxation. eNOS activation is stimulated by various mechanical forces, such as shear stress. Several studies have shown that local cooling of the human finger causes strong vasoconstriction, followed after several minutes by cold-induced vasodilation (CIVD). However, the role played by endothelial cells (ECs) in blood vessel regulation in respond to cold temperatures is not fully understood. In this study, we found that low temperature alone does not significantly increase or decrease eNOS activation in ECs. We further found that the combination of shear stress with temperature change leads to a significant increase in eNOS activation at 37 °C and 28 °C, and a decrease at 4 °C. These results show that ECs play an important role in blood vessel regulation under shear stress and low temperature. 相似文献