Life History of Kampimodromus aberrans as a predator of Phytoptus avellanae (Acari: Phytoseiidae, Phytoptidae)

The biology of Kampimodromus aberrans (Oudemans), a predator of the big bud mite, Phytoptus avellanae Nalepa, was studied under laboratory conditions. All experiments were conducted on hazelnut leaf discs in an incubator at 25 ± 1 °C, with 16:8 h L:D, at an average daily relative air humidity of 76%. Observations were made twice daily for the immature stages and daily for the adults to determine developmental time, survival and fecundity. The mean developmental time for females was 6.90 days and for males was 7.10 days, and mean adult longevity for females was 11.67 days. The mean total and daily egg production were 12.67 and 1.85 eggs, respectively. The net reproduction rate (R₀) was 7.09 females/female, intrinsic rate of increase (r_m) was 0.153 female/female/day and mean generation time (T₀) was 12.80 days. The mortality rate of immature stages was 0.66% and the sex ratio was 0.51 female.     

The Effects of Copper Sulfate on Liver Histology and Biochemical Parameters of Term Ross Broiler Chicks

Copper is an essential trace element that is extremely toxic to organisms and organs at high doses. We have investigated the histological and biochemical effects of a toxic dose of copper sulfate on the liver of term Ross broiler chicks. Fertilized eggs were divided into three groups: experimental, injected with 50 mcg/0.1 ml copper sulfate in the air chambers on day 1; sham, injected with 0.1 ml saline; and control, no injection. Term chicks were killed and their livers investigated histologically, with hematoxylin–eosin-stained sections examined under light microscopy, and biochemically, for malondialdehyde and glutathione levels. Histological examinations showed copper-treated samples with granular degeneration and necrosis of hepatocytes and impairment to the cell lining of the remark cords. The samples had a congestive appearance, with blood in the vena centralis and sinusoids, slight connective tissue increase, and lymphocyte infiltration. Control and sham group sections had normal appearances. As oxidative damage parameters, in the copper-treated group, malondialdehyde levels were increased and glutathione levels decreased. In the sham and control groups, there were no significant differences. At this toxic dose, copper sulfate shows oxidative damage according to the histology of term chick liver that are confirmed biochemically by the changes in malondialdehyde and glutathione levels.     

Comparison of aerobic and anaerobic degradation of municipal solid waste in bioreactor landfills

Two landfill bioreactors were operated under aerobic and anaerobic conditions in a thermo-insulated room at a constant temperature of 32 °C. Reactors were filled with 19.5 kg of shredded synthetic solid waste prepared according to the average municipal solid waste compositions determined in Istanbul and operated under wet-tomb management strategy by using leachate recirculation. Aerobic conditions in the reactor were developed by using an air compressor. The results of experiments indicated that aerobic reactor had higher organic, nitrogen, phosphorus and alkali metal removal efficiencies than the anaerobic one. Furthermore, stabilization time considerably decreased when using aerobic processes with leachate recirculation compared to the anaerobic system with the same recirculation scheme.     

WCA: A Weighted Clustering Algorithm for Mobile Ad Hoc Networks

In this paper, we propose an on-demand distributed clustering algorithm for multi-hop packet radio networks. These types of networks, also known as ad hoc networks, are dynamic in nature due to the mobility of nodes. The association and dissociation of nodes to and from clusters perturb the stability of the network topology, and hence a reconfiguration of the system is often unavoidable. However, it is vital to keep the topology stable as long as possible. The clusterheads, form a dominant set in the network, determine the topology and its stability. The proposed weight-based distributed clustering algorithm takes into consideration the ideal degree, transmission power, mobility, and battery power of mobile nodes. The time required to identify the clusterheads depends on the diameter of the underlying graph. We try to keep the number of nodes in a cluster around a pre-defined threshold to facilitate the optimal operation of the medium access control (MAC) protocol. The non-periodic procedure for clusterhead election is invoked on-demand, and is aimed to reduce the computation and communication costs. The clusterheads, operating in dual power mode, connects the clusters which help in routing messages from a node to any other node. We observe a trade-off between the uniformity of the load handled by the clusterheads and the connectivity of the network. Simulation experiments are conducted to evaluate the performance of our algorithm in terms of the number of clusterheads, reaffiliation frequency, and dominant set updates. Results show that our algorithm performs better than existing ones and is also tunable to different kinds of network conditions.     

Evaluation of the natural killer cytotoxicity and the levels of cytokines in rats with type I diabetes mellitus

Type I diabetes mellitus (insulin-dependent DM = IDDM) is a chronic disease characterized by specific destruction of pancreatic beta cells, resulting in an absolute lack of insulin. Immune mechanisms, genetic susceptibility, and environmental factors are all implicated in the pathogenesis of Type 1 diabetes. This study was aimed at determining the efficiency of cytokines, natural killer (NK) cells in the pathophysiology of IDDM. Therefore, we evaluated the plasma levels of cytokines by specific enzyme-linked immunosorbent assay (ELISA) and the cytotoxicity activity of NK cells by anti-candididal index in rats with type I diabetes. We found that the cytotoxicity activity of NK cells in IDDM groups significantly decreased compared to the control groups. The levels of interferon-gamma (IFN-gamma) in IDDM groups were slightly higher than in healthy controls. These results indicate that the changes of T H1 type cytokines such as IFN-gamma and NK cell activity can play a role in the etiology of IDDM. The data may provide new strategies for the treatment of IDDM.     

PROGRESS STUDY: Progression of chronic kidney disease in children and heat shock proteins

Various molecular and cellular processes are involved in renal fibrosis, such as oxidative stress, inflammation, endothelial cell injury, and apoptosis. Heat shock proteins (HSPs) are implicated in the progression of chronic kidney disease (CKD). Our aim was to evaluate changes in urine and serum HSP levels over time and their relationships with the clinical parameters of CKD in children. In total, 117 children with CKD and 56 healthy children were examined. The CKD group was followed up prospectively for 24 months. Serum and urine HSP27, HSP40, HSP47, HSP60, HSP70, HSP72, and HSP90 levels and serum anti-HSP60 and anti-HSP70 levels were measured by ELISA at baseline, 12 months, and 24 months. The urine levels of all HSPs and the serum levels of HSP40, HSP47, HSP60, HSP70, anti-HSP60, and anti-HSP70 were higher at baseline in the CKD group than in the control group. Over the months, serum HSP47 and HSP60 levels steadily decreased, whereas HSP90 and anti-HSP60 levels steadily increased. Urine HSP levels were elevated in children with CKD; however, with the exception of HSP90, they decreased over time. In conclusion, our study demonstrates that CKD progression is a complicated process that involves HSPs, but they do not predict CKD progression. The protective role of HSPs against CKD may weaken over time, and HSP90 may have a detrimental effect on the disease course.Supplementary InformationThe online version contains supplementary material available at 10.1007/s12192-021-01239-9.     

Alarming evidence of widespread mite extinctions in the shadows of plant,insect and vertebrate extinctions

This paper, which addresses the issue of the extinction of mite species at the global scale for the first time, highlights mite diversity, assesses the evidence for an extinction process, discusses contributing factors and estimates losses. The ~1 250 000 mite species occupy an enormous variety of terrestrial and freshwater ecosystems from the equator to the polar regions and to high altitudes. Some groups provide essential ecosystem services, including the incorporation of organic matter into the soil. The maintenance of mite diversity is inextricably linked to the continuance of floristic diversity, habitat complexity and insect diversity. The majority of mite species are assumed to be in the tropical rainforests, of which >50% has been destroyed or severely degraded. Most biodiversity hotspots are in tropical forests; endemic phytoseiid mite species are at least 17 times more concentrated in the hotspots than outside. Habitat destruction and degradation continue on an enormous scale, with increasing human population growth and resource consumption the overarching drivers of extinction. Moreover, climate change is likely to be worsening the effects of the other drivers at an increasing rate. The small body of direct evidence and a considerable body of indirect evidence strongly suggest the continuing, widespread extinction of mite species. Based on estimates of overall biodiversity loss, ~15% of mite species were likely to have become extinct by 2000, with losses currently expected to increase by between 0.6% and 6.0% by 2060. More detailed information on both spatial differences in mite assemblages and anthropogenic threats worldwide is crucial because they underpin the total number of species and their vulnerability to extinction, respectively. The rapid expansion of the protected area estate to capture the maximum possible area of ecosystem heterogeneity, especially in the biodiversity hotspots, is essential, as is best practice management of these areas.     

An ultrastructural study of the relationship between the mite Floracarus perrepae Knihinicki & Boczek (Acariformes: Eriophyidae) and the fern Lygodium microphyllum (Lygodiaceae)

Abstract  The ultrastructure of the mite Floracarus perrepae was investigated in relation to its host, Lygodium microphyllum , the Old World climbing fern. Floracarus perrepae has been suggested as a means of biological control for the fern, which is an aggressive weed in tropical areas. Feeding by the mite induces a change in the size of epidermal cells, and cell division is stimulated by mite feeding, causing the leaf margin to curl over into a roll with two to three windings. The enlarged epidermal layer greatly increases its cytoplasmic contents, which become a nutritive tissue for the mite and its progeny. Damage by the mite ultimately debilitates the fern. The structure and depth of stylet penetration by the mite, and the thickness of the epidermal cell wall of L. microphyllum , do not appear to account for the mite's differential ability to induce leaf rolling in its co-adapted host from south-east Queensland but not in the invasive genotype of the fern in Florida.