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101.
Soil and total ecosystem respiration in agricultural fields: effect of soil and crop type 总被引:22,自引:0,他引:22
A study was made of the effect of soil and crop type on the soil and total ecosystem respiration rates in agricultural soils in southern Finland. The main interest was to compare the soil respiration rates in peat and two different mineral soils growing barley, grass and potato. Respiration measurements were conducted during the growing season with (1) a closed-dynamic ecosystem respiration chamber, in which combined plant and soil respiration was measured and (2) a closed-dynamic soil respiration chamber which measured only the soil and root-derived respiration. A semi-empirical model including separate functions for the soil and plant respiration components was used for the total ecosystem respiration (TER), and the resulting soil respiration parameters for different soil and crop types were compared. Both methods showed that the soil respiration in the peat soil was 2–3 times as high as that in the mineral soils, varying from 0.11 to 0.36 mg (CO2) m–2 s–1 in the peat soil and from 0.02 to 0.17 mg (CO2) m–2 s–1 in the mineral soils. The difference between the soil types was mainly attributed to the soil organic C content, which in the uppermost 20 cm of the peat soil was 24 kg m–2, being about 4 times as high as that in the mineral soils. Depending on the measurement method, the soil respiration in the sandy soil was slightly higher than or similar to that in the clay soil. In each soil type, the soil respiration was highest on the grass plots. Higher soil respiration parameter values (Rs0, describing the soil respiration at a soil temperature of 10°C, and obtained by modelling) were found on the barley than on the potato plots. The difference was explained by the different cultivation history of the plots, as the potato plots had lain fallow during the preceding summer. The total ecosystem respiration followed the seasonal evolution in the leaf area and measured photosynthetic flux rates. The 2–3-fold peat soil respiration term as compared to mineral soil indicates that the cultivated peat soil ecosystem is a strong net CO2 source. 相似文献
102.
Cremades N Cohen SI Deas E Abramov AY Chen AY Orte A Sandal M Clarke RW Dunne P Aprile FA Bertoncini CW Wood NW Knowles TP Dobson CM Klenerman D 《Cell》2012,149(5):1048-1059
Here, we use single-molecule techniques to study the aggregation of α-synuclein, the protein whose misfolding and deposition is associated with Parkinson's disease. We identify a conformational change from the initially formed oligomers to stable, more compact proteinase-K-resistant oligomers as the key step that leads ultimately to fibril formation. The oligomers formed as a result of the structural conversion generate much higher levels of oxidative stress in rat primary neurons than do the oligomers formed initially, showing that they are more damaging to cells. The structural conversion is remarkably slow, indicating a high kinetic barrier for the conversion and suggesting that there is a significant period of time for the cellular protective machinery to operate and potentially for therapeutic intervention, prior to the onset of cellular damage. In the absence of added soluble protein, the assembly process is reversed and fibrils disaggregate to form stable oligomers, hence acting as a source of cytotoxic species. 相似文献
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105.
Mari Nyyssönen Anu Kapanen Reetta Piskonen Tuomas Lukkari Merja Itävaara 《Applied microbiology and biotechnology》2009,84(1):169-182
A small-scale functional gene array containing 15 functional gene probes targeting aliphatic and aromatic hydrocarbon biodegradation
pathways was used to investigate the effect of a pilot-scale air sparging and nutrient infiltration treatment on hydrocarbon
biodegradation in creosote-contaminated groundwater. Genes involved in the different phases of polycyclic aromatic hydrocarbon
(PAH) biodegradation were detected with the functional gene array in the contaminant plume, thus indicating the presence of
intrinsic biodegradation potential. However, the low aerobic fluorescein diacetate hydrolysis, the polymerase chain reaction
(PCR) amplification of 16S rRNA genes closely similar to sulphate-reducing and denitrifying bacteria and the negligible decrease
in contaminant concentrations showed that aerobic PAH biodegradation was limited in the anoxic groundwater. Increased abundance
of PAH biodegradation genes was detected by functional gene array in the monitoring well located at the rear end of the biostimulated
area, which indicated that air sparging and nutrient infiltration enhanced the intrinsic, aerobic PAH biodegradation. Furthermore,
ten times higher naphthalene dioxygenase gene copy numbers were detected by real-time PCR in the biostimulated area, which
was in good agreement with the functional gene array data. As a result, functional gene array analysis was demonstrated to
provide a potential tool for evaluating the efficiency of the bioremediation treatment for enhancing hydrocarbon biodegradation
in field-scale applications.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
106.
The chromosomal locus NP_636946 of Xanthomonas campestris DSM 3586 (ATCC 33913) which was earlier presumed to encode a quinoprotein glucose dehydrogenase has been cloned, expressed in Escherichia coli and the recombinant enzyme has been characterised. It was found to have no glucose dehydrogenase activity but to be active on many different polyols and diols, aliphatic alcohols, certain aldonic acids and amino-sugars. The product of d-gluconic acid oxidation was 5-keto-d-gluconic acid. The enzyme differs from polyol/gluconate dehydrogenases found in Gluconobacter by its single-chain architecture, different substrate specificity and much higher (20- to 30-fold) expression level in E.coli. 相似文献
107.
We study the joint evolution of dispersal and specialization concerning resource usage in a mechanistically underpinned structured discrete-time metapopulation model. We show that dispersal significantly affects the evolution of specialization and that specialization is a key factor that determines the possibility of evolutionary branching in dispersal propensity. Allowing both dispersal propensity and specialization to evolve as a consequence of natural selection is necessary in order to understand the evolutionary dynamics. The joint evolution of dispersal and specialization forms a natural evolutionary path leading to the coexistence of generalists and specialists. We show that in this process, the number of different patch types and the resource distribution are essential. 相似文献
108.
VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling 总被引:3,自引:0,他引:3
Tammela T Zarkada G Nurmi H Jakobsson L Heinolainen K Tvorogov D Zheng W Franco CA Murtomäki A Aranda E Miura N Ylä-Herttuala S Fruttiger M Mäkinen T Eichmann A Pollard JW Gerhardt H Alitalo K 《Nature cell biology》2011,13(10):1202-1213
Angiogenesis, the growth of new blood vessels, involves specification of endothelial cells to tip cells and stalk cells, which is controlled by Notch signalling, whereas vascular endothelial growth factor receptor (VEGFR)-2 and VEGFR-3 have been implicated in angiogenic sprouting. Surprisingly, we found that endothelial deletion of Vegfr3, but not VEGFR-3-blocking antibodies, postnatally led to excessive angiogenic sprouting and branching, and decreased the level of Notch signalling, indicating that VEGFR-3 possesses passive and active signalling modalities. Furthermore, macrophages expressing the VEGFR-3 and VEGFR-2 ligand VEGF-C localized to vessel branch points, and Vegfc heterozygous mice exhibited inefficient angiogenesis characterized by decreased vascular branching. FoxC2 is a known regulator of Notch ligand and target gene expression, and Foxc2(+/-);Vegfr3(+/-) compound heterozygosity recapitulated homozygous loss of Vegfr3. These results indicate that macrophage-derived VEGF-C activates VEGFR-3 in tip cells to reinforce Notch signalling, which contributes to the phenotypic conversion of endothelial cells at fusion points of vessel sprouts. 相似文献
109.
Most proteins must remain soluble in the cytosol in order to perform their biological functions. To protect against undesired protein aggregation, living cells maintain a population of molecular chaperones that ensure the solubility of the proteome. Here we report simulations of a lattice model of interacting proteins to understand how low concentrations of passive molecular chaperones, such as small heat-shock proteins, suppress thermodynamic instabilities in protein solutions. Given fixed concentrations of chaperones and client proteins, the solubility of the proteome can be increased by tuning the chaperone–client binding strength. Surprisingly, we find that the binding strength that optimizes solubility while preventing irreversible chaperone binding also promotes the formation of weakly bound chaperone oligomers, although the presence of these oligomers does not significantly affect the thermodynamic stability of the solution. Such oligomers are commonly observed in experiments on small heat-shock proteins, but their connection to the biological function of these chaperones has remained unclear. Our simulations suggest that this clustering may not have any essential biological function, but rather emerges as a natural side-effect of optimizing the thermodynamic stability of the proteome. 相似文献
110.