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11.
Tsai MS Yang YL Wang AH Wang LS Lu DC Liou CH Hsieh LY Wu CJ Cheng MF Shi ZY Lo HJ 《Mycopathologia》2012,174(2):121-130
A total of 35 Trichosporon isolates were collected from the Taiwan Surveillance of Antimicrobial Resistance of Yeasts (TSARY) project from 1999 to 2006, and their identifications as well as drug susceptibilities were determined. The most frequently isolated species was T. asahii (62.9%), and the most common clinical sample that yielded Trichosporon isolates was urine (37.1%). The etiology of all seven invasive trichosporonosis was T. asahii. For the 22 T. asahii isolates, the MIC(50) and MIC(90) for amphotericin B were 0.25 and 1 μg/mL, respectively. Those for fluconazole were 2 and 4 μg/mL, respectively, and for voriconazole 0.031 and 0.063 μg/mL, respectively. When the intraclass correlation coefficients (ICCs) and agreements were calculated, we found that the MICs of fluconazole obtained from different methods were similar and the inter-method discrepancies were low. Nevertheless, no unanimous MIC of amphotericin B and voriconazole was obtained among different methods. 相似文献
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Wang LC Severinghaus LL Chen CT Liu LY Pan CH Huang D Lee HY Lir JT Chin SC Pu CE Wang CH 《The Journal of heredity》2008,99(2):187-192
Molecular sexing of the diversified avian family Strigidae is difficult. Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Here we describe a simple method for sexing a variety of Strigidae species using oligonucleotide microarrays, on which several sex-specific probes operated complementarily or in concert. The sex of 8 owl species was identified clearly on the microarrays through sequence recognition. This sequence-directed method can be easily applied to a wider range of Strigidae species. 相似文献
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Levels of malondialdehyde in the gastric juice: Its association with Helicobacter pylori infection and stomach diseases 下载免费PDF全文
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The high‐polarity β‐phase poly(vinylidene difluoride) (β‐PVDF), which has all trans conformation with F and H atoms located on the opposite sides of the polymer backbone, is demonstrated to be a promising artificial solid‐electrolyte interphase coating on both Cu and Li metal anodes for dendrite‐free Li deposition/stripping and enhanced cycling performance. A thin (≈4 µm) β‐PVDF coating on Cu enables uniform Li deposition/stripping at high current densities up to 5 mA cm?2, Li‐plating capacity loadings of up to 4 mAh cm?2, and excellent cycling stability over hundreds of cycles under practical conditions (1 mA cm?2 with 2 mAh cm?2). Full cells containing an LiFePO4 cathode and an anode of either β‐PVDF coated Cu or Li also exhibit excellent cycling stability. The profound effects of the high‐polarity PVDF coating on dendrite suppression are attributed to the electronegative F‐rich interface that favors layer‐by‐layer Li deposition. This study offers a new strategy for the development of dendrite‐free metal anode technology. 相似文献
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Effects of human amniotic membrane grafts combined with marrow mesenchymal stem cells on healing of full-thickness skin defects in rabbits 总被引:1,自引:0,他引:1
Sung Soo Kim Chang Keun Song Sung Keun Shon Kyu Yeol Lee Chul Hong Kim Myung Jin Lee Lih Wang 《Cell and tissue research》2009,336(1):59-66
We have investigated the wound-healing effects of mesenchymal stem cells (MSCs) in combination with human amniotic membrane
(HAM) when grafted into full-thickness skin defects of rabbits. Five defects in each of four groups were respectively treated
with HAM loaded with autologous MSCs (group A), HAM loaded with allologous MSCs (group B), HAM with injected autologous MSCs
(group C), and HAM with injected allologous MSCs (group D). The size of the wounds was calculated for each group at 7, 12,
and 15 days after grafting. The wounds were subsequently harvested at 25 days after grafting. Sections stained with hematoxylin
and eosin were used to determine the quality of wound healing, as based on the characteristics and amount of granulated tissue
in the epidermal and dermal layers. Groups A and B showed the most pronounced effect on wound closure, with statistically
significant improvement in wound healing being seen on post-operative days 7, 12, and 15. Although a slight trend toward improved
wound healing was seen in group A compared with group B, no statistically significant difference was found at any time point
between the two groups. Histological examination of healed wounds from groups A and B showed a thin epidermis with mature
differentiation and collagen bundle deposition plus recovered skin appendages in the dermal layer. In contrast, groups C and
D showed thickened epidermis with immature epithelial cells and increased fibroblast proliferation with only partially recovered
skin appendages in the dermal layer. Thus, the graft of HAM loaded with MSCs played an effective role during the healing of
skin defects in rabbits, with no significant difference being observed in wound healing between autologous and allologous
MSC transplantation.
This study was supported by research funds from Dong-A University. 相似文献
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Hyun Kyoung Lee Lih Wang Yoon Kyung Shin Duk Joon Suh 《Biochemical and biophysical research communications》2009,382(2):410-414
Interleukin-6 plays an important role in peripheral nerve regeneration. We recently reported that IL-6 targets Schwann cells in the peripheral nerve for its function. In this study, we analyzed genes whose expression is regulated by IL-6 in a cell line derived from Schwann cells, the peripheral glia, using the Illumina gene microarray. At measurements 3 and 12 h after IL-6 treatment, 35 genes were found to be upregulated by IL-6. Most upregulated genes were proinflammatory genes that are known to be induced in inflammatory conditions. Interestingly, the expression of immunoproteasome subunits was upregulated by IL-6 in Schwann cells. Treatment with forskolin, an agent that mimics axonal signaling, suppressed the expression of IL-6-inducible genes. Finally, we found for the first time that sciatic nerve injury induced immunoproteasome expression in vivo. These findings indicate that IL-6 is involved in peripheral nerve regeneration by regulating proinflammatory signaling in Schwann cells. 相似文献
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Tatsuhisa Takahashi Taiji Nagaoka Hirotaka Yanagida Tadashi Saitoh Akira Kamiya Travis Hein Lih Kuo Akitoshi Yoshida 《Journal of Biorheology》2009,23(2):77-86
To quantitatively assess the arteriovenous distribution of hemodynamic parameters throughout the microvascular network of
the human retina, we constructed a retinal microcirculatory model consisting of a dichotomous symmetric branching system.
This system is characterized by a diameter exponent of 2.85, instead of 3 as dictated by Murray’s law, except for the capillary
networks. The value of 2.85 was the sum of a fractal dimension (1.70) and a branch exponent (1.15) of the retinal vasculature.
Following the feeding artery (central retinal artery), each bifurcation was recursively developed at a distance of an individual
branch length [L(r) = 7.4r
1.15] by a centrifugal scheme. The venular tree was formed in the same way. Using this model, we evaluated hemodynamic parameters,
including blood pressure, blood flow, blood velocity, shear rate, and shear stress, within the retinal microcirculatory network
as a function of vessel diameter. The arteriovenous distributions of blood pressure and velocity in the simulation were consistent
with in vivo measurements in the human retina and other vascular beds of small animals. We therefore conclude that the current
theoretical model was useful for quantifying hemodynamics as a function of vessel diameter within the retinal microvascular
network. 相似文献
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Ariffin N Abdullah R Rashdan Muad M Lourdes J Emran NA Ismail MR Ismail I Fadzil MF Ling KL Siddiqui Y Amir AA Berahim Z Husni Omar M 《Plasmid》2011,66(3):136-143
Polyhydroxybutyrate-co-hydroxyvalerate (PHBV) is a polyhydroxyalkanoate (PHA) bioplastic group with thermoplastic properties is thus high in quality and can be degradable. PHBV can be produced by bacteria, but the process is not economically competitive with polymers produced from petrochemicals. To overcome this problem, research on transgenic plants has been carried out as one of the solutions to produce PHBV in economically sound alternative manner. Four different genes encoded with the enzymes necessary to catalyze PHBV are bktB, phaB, phaC and tdcB. All the genes came with modified CaMV 35S promoters (except for the tdcB gene, which was promoted by the native CaMV 35S promoter), nos terminator sequences and plastid sequences in order to target the genes into the plastids. Subcloning resulted in the generation of two different orientations of the tdcB, pLMIN (left) and pRMIN (right), both 17.557 and 19.967 kb in sizes. Both plasmids were transformed in immature embryos (IE) of oil palm via Agrobacterium tumefaciens. Assays of GUS were performed on one-week-old calli and 90% of the calli turned completely blue. This preliminary test showed positive results of integration. Six-months-old calli were harvested and RNA of the calli were isolated. RT-PCR was used to confirm the transient expression of PHBV transgenes in the calli. The bands were 258, 260, 315 and 200 bp in size for bktB, phaB, phaC and tdcB transgenes respectively. The data obtained showed that the bktB, phaB, phaC and tdcB genes were successfully integrated and expressed in the oil palm genome. 相似文献
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