全文获取类型
收费全文 | 166篇 |
免费 | 16篇 |
出版年
2022年 | 1篇 |
2019年 | 1篇 |
2016年 | 1篇 |
2015年 | 4篇 |
2014年 | 8篇 |
2013年 | 7篇 |
2012年 | 18篇 |
2011年 | 9篇 |
2010年 | 17篇 |
2009年 | 12篇 |
2008年 | 13篇 |
2007年 | 9篇 |
2006年 | 5篇 |
2005年 | 7篇 |
2004年 | 4篇 |
2003年 | 7篇 |
2002年 | 3篇 |
2001年 | 3篇 |
2000年 | 8篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 3篇 |
1996年 | 3篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1980年 | 1篇 |
1972年 | 3篇 |
1969年 | 1篇 |
1967年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有182条查询结果,搜索用时 656 毫秒
71.
72.
Pratibha Misra Neha Gupta Dibya D. Toppo Vibha Pandey Manoj Kumar Mishra Rakesh Tuli 《Plant Cell, Tissue and Organ Culture》2010,100(2):189-197
Leaf explants of the second or third node were collected from field-grown elite Jatropha curcas trees and incubated in Murashige and Skoog’s (Physiol Plant 15:473–497, 1962) medium supplemented with growth regulators. Direct shoot organogenesis was induced when explants were incubated in a medium containing 0.5 mg l?1 benzyladenine (BA) and 0.1 mg l?1 indolebutyric acid (IBA). A maximum of seven shoot buds differentiated within 6 weeks of culture incubation. Indirect shoot organogenesis was obtained when explants were incubated in the medium supplemented with 0.5 mg l?1 BA along with 1.0 mg l?1 each of 2,4-dichlorophenoxyacetic acid (2,4-D) and indoleacetic acid (IAA). A pulse treatment of 0.5 mg l?1 thidiazurone (TDZ) and 0.1 mg l?1 IBA for 5 days was necessary for shoot organogenesis in green compact callus before subculture into 0.5 mg l?1 BA and 0.1 mg l?1 IBA containing medium. Leaf explants of J. curcas, collected from the field, contained endophytic bacterial contamination, which expressed itself after 2–3 subcultures. These bacteria were cultured and identified as Enterobacter ludwigii. After staining, these were found as gram-negative bacteria. Their sensitivity against different antibiotics has been tested by culturing them with different antibiotic stabs for 72 h. Finally, Augmentin® was found as the most effective and suitable antibiotic which not only controlled the bacteria within 2–3 subcultures but also supported the regeneration system and growth of the regenerated shoots and such cultures have been grown for a long-term of over 2 years without any contamination. 相似文献
73.
74.
Sudip K. Ghosh Katrina L. Van Dellen Anirban Chatterjee Tuli Dey Rashidul Haque Phillips W. Robbins John Samuelson 《PLoS neglected tropical diseases》2010,4(7)
Background
The infectious and diagnostic form of Entamoeba histolytica (Eh), cause of amebic dysentery and liver abscess, is the quadranucleate cyst. The cyst wall of Entamoeba invadens (Ei), a model for Eh, is composed of chitin fibrils and three sets of chitin-binding lectins that cross-link chitin fibrils (multivalent Jacob lectins), self-aggregate (Jessie lectins), and remodel chitin (chitinase). The goal here was to determine how well the Ei model applies to Entamoeba cysts from humans.Methods/Results
An Eh Jacob lectin (EhJacob2) has three predicted chitin-binding domains surrounding a large, Ser-rich spacer. Recombinant EhJacob2 made in transfected Eh trophozoites binds to particulate chitin. Sequences of PCR products using primers flanking the highly polymorphic spacer of EhJacob2 may be used to distinguish Entamoeba isolates. Antibodies to the EhJacob2, EhJessie3, and chitinase each recognize cyst walls of clinical isolates of Entamoeba. While numerous sera from patients with amebic intestinal infections and liver abscess recognize recombinant EhJacob1 and EhJessie3 lectins, few of these sera recognize recombinant EhJacob2.Conclusions/Significance
The EhJacob2 lectin binds chitin and is polymorphic, and Jacob2, Jessie3, and chitinase are present in cyst walls of clinical isolates of Entamoeba. These results suggest there are substantial similarities between cysts of the human pathogen (Eh) and the in vitro model (Ei), even though there are quantitative and qualitative differences in their chitin-binding lectins. 相似文献75.
O. P. Sidhu Sanjay Annarao Uday Pathre S. K. Snehi S. K. Raj Raja Roy Rakesh Tuli C. L. Khetrapal 《Planta》2010,232(1):85-93
Alterations in the anatomical structures, sap translocation and metabolic profiles in Jatropha curcas L. (Euphorbiaceae), infected with Jatropha mosaic virus (JMV) have been investigated using MRI and HR-MAS NMR spectroscopy. The contrast of MRI images distinguishes abnormalities
in anatomical structures of infected and healthy stem. The HR-MAS NMR spectroscopic analysis indicated that viral infection
significantly affected the plant metabolism. Higher accumulation of TCA cycle intermediates, such as citrate and malate, in
JMV-infected plants suggested a higher rate of respiration. The respiration rate was more than twofold as compared to healthy
ones. The viral stress also significantly increases the concentrations of alanine, arginine, glutamine, valine, GABA and choline
as compared to healthy ones. Microscopic examination revealed severe hyperplasia caused by JMV with a considerable reduction
in the size of stem cells. Lower concentration of glucose and sucrose in viral-infected stem tissues indicates decreased translocation
of photosynthates from leaves to stem due to hyperplasia caused by JMV. The MR images distinguished stele, cortical and pith
regions of JMV-infected and healthy stems. Contrast of T1- and T2-weighted images showed significant differences in the spatial distribution of water, lipids and macromolecules in virus-infected
and healthy stem tissues. The results demonstrated the value of MRI and HR-MAS NMR spectroscopy in studying viral infection
and metabolic shift in plants. The present methodology may help in better understanding the metabolic alterations during biotic
stress in other plant species of agricultural and commercial importance. 相似文献
76.
Rakesh Sarin Ravindra KumarBhawana Srivastav S.K. PuriD.K. Tuli R.K. MalhotraAnand Kumar 《Bioresource technology》2009,100(12):3022-3028
Synthesis of surrogate molecules is particularly useful for generating in sight of structural-activity relationships, understanding processes and improving the performance. In order to improve upon the physico-chemical properties of biodiesel, methyl, ethyl, isopropyl and n-butyl esters of β-branched fatty acid have been synthesized, initiating from β-branched alcohols. β-Branched alcohols upon oxidation gave corresponding acids, which were converted to their esters. The synthesized esters have substantially better oxidative stability, exhibited by Rancimat oxidation induction period of more than 24 h. The cloud point of synthesized esters is <−36 °C, pour point is <−42 °C and CFPP is <−21 °C, which is substantially better than fatty acid methyl esters. Besides achieving the objective of better oxidative stability and improved low temperature properties, the synthesized surrogate esters have viscosity in the range of 4.2–4.6 cSt at 40 °C, meeting the international diesel and biodiesel standards. The cetane number of synthesized esters is 62–69, which is much better than diesel and biodiesel. The blends of the synthesized esters in diesel at 5% and 10% meet Indian standards of diesel. 相似文献
77.
78.
79.
Upadhyay SK Saurabh S Singh R Rai P Dubey NK Chandrashekar K Negi KS Tuli R Singh PK 《The protein journal》2011,30(6):374-383
A lectin was purified from the leaves of Allium altaicum and corresponding gene was cloned. The lectin namely Allium altaicum agglutinin (AAA) was ~24 kDa homodimeric protein and similar to a typical garlic leaf lectin. It was synthesized as 177 amino
acid residues pre-proprotein, which consisted of 28 and 43 amino acid long N and C-terminal signal peptides, respectively.
The plant expressed this protein more in scapes and flowers in comparison to the bulbs and leaves. Hemagglutination activity
(with rabbit erythrocytes) was 1,428 fold higher as compared to Allium sativum leaf agglutinin (ASAL) although, the insecticidal activity against cotton aphid (Aphis gossypii) was relatively low. Glycan array revealed that AAA had higher affinity towards GlcAb1-3Galb as compared to ASAL. Homology
analysis showed 57–94% similarity with other Allium lectins. The mature protein was expressed in E. coli as a fusion with SUMO peptide in soluble and biologically active form. Recombinant protein retained high hemagglutination activity. 相似文献