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181.
This review outlines the various methods available for submitting sequence data to the EMBL Nucleotide Sequence Database.
Depending on the type of sequence data and the facilities available to the submitter, one method may be more suitable than
another. Recent developments have been the World Wide Web submission tool, procedures for bulk submissions and genome projects. 相似文献
182.
M. Sujatha M. Lakshminarayana M. Tarakeswari P. K. Singh Rakesh Tuli 《Plant cell reports》2009,28(6):935-946
Castor (cv. DCS-9) has been transformed through Agrobacterium-mediated and particle gun bombardment methods using appropriate vectors containing the Bt chimeric gene cry1EC driven by enhanced 35S promoter. About 81 and 12 putative transformants were regenerated following selection on hygromycin
and kanamycin, respectively. Southern analysis of DNA extracted from T0 plants confirmed integration of the introduced gene in castor genome. The integration and inheritance of the introduced genes
was demonstrated up to T4 generation by PCR and Southern analysis. Southern analysis of two events having single and two copies showed the same pattern
of integration in the subsequent generations. Insect feeding experiments conducted in the laboratory by releasing neonate
larvae of castor semilooper and S. litura on leaf tissues excised from transgenic and control plants showed varying degrees of larval mortality and slow growth in
larvae fed on transgenic leaf tissue. Field bioassays against Spodoptera litura and castor semilooper conducted for eight events in T1–T4 generations under net confinement were more informative and events conferring resistance to the two major defoliators were
identified. 相似文献