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71.
Preferential Colonization of Solanum tuberosum L. Roots by the Fungus Glomus intraradices in Arable Soil of a Potato Farming Area
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Patrizia Cesaro Diederik van Tuinen Andrea Copetta Odile Chatagnier Graziella Berta Silvio Gianinazzi Guido Lingua 《Applied microbiology》2008,74(18):5776-5783
The symbiosis between plant roots and arbuscular mycorrhizal (AM) fungi has been shown to affect both the diversity and productivity of agricultural communities. In this study, we characterized the AM fungal communities of Solanum tuberosum L. (potato) roots and of the bulk soil in two nearby areas of northern Italy, in order to verify if land use practices had selected any particular AM fungus with specificity to potato plants. The AM fungal large-subunit (LSU) rRNA genes were subjected to nested PCR, cloning, sequencing, and phylogenetic analyses. One hundred eighty-three LSU rRNA sequences were analyzed, and eight monophyletic ribotypes, belonging to Glomus groups A and B, were identified. AM fungal communities differed between bulk soil and potato roots, as one AM fungal ribotype, corresponding to Glomus intraradices, was much more frequent in potato roots than in soils (accounting for more than 90% of sequences from potato samples and less than 10% of sequences from soil samples). A semiquantitative heminested PCR with specific primers was used to confirm and quantify the AM fungal abundance observed by cloning. Overall results concerning the biodiversity of AM fungal communities in roots and in bulk soils from the two studied areas suggested that potato roots were preferentially colonized by one AM fungal species, G. intraradices. 相似文献
72.
25S rDNA-based molecular monitoring of glomalean fungi in sewage sludge-treated field plots 总被引:4,自引:0,他引:4
Jacquot-Plumey E van Tuinen D Chatagnier O Gianinazzi S Gianinazzi-Pearson V 《Environmental microbiology》2001,3(8):525-531
Recycling of sewage wastes in agriculture is likely to affect the biological activity of soils through contamination of ecosystems by pathogens and metallic or organic micropollutants. The impact of sewage sludge spreading under field conditions on arbuscular mycorrhiza (AM) formation by a community of glomalean fungi was evaluated using a nested polymerase chain reaction (PCR) and discriminating primers based on 25S rDNA polymorphisms to detect different fungal species within root systems. Medicago truncatula was grown in soil of field plots amended or not with a composted sewage sludge, spiked or not with organic or metallic micropollutants. Overall AM development in roots decreased with sewage sludge application, and the relative abundance of five AM fungal morphotypes in root fragments was modified by the input of composted sludges. Sewage sludge spiked or not with organic pollutants had a generally positive effect on the relative diversity of AM fungal populations in planta , whereas after spreading of the sludge spiked with metallic pollutants, no variation was observed in the abundance of different species. 相似文献
73.
The early history of modern birds inferred from DNA sequences of nuclear and mitochondrial ribosomal genes 总被引:17,自引:0,他引:17
The traditional view of avian evolution places ratites and tinamous at the base of the phylogenetic tree of modern birds (Neornithes). In contrast, most recent molecular studies suggest that neognathous perching birds (Passeriformes) compose the oldest lineage of modern birds. Here, we report significant molecular support for the traditional view of neognath monophyly based on sequence analyses of nuclear and mitochondrial DNA (4.4 kb) from every modern avian order. Phylogenetic analyses further show that the ducks and gallinaceous birds are each other's closest relatives and together form the basal lineage of neognathous birds. To investigate why other molecular studies sampling fewer orders have reached different conclusions regarding neognath monophyly, we performed jackknife analyses on our mitochondrial data. Those analyses indicated taxon-sampling effects when basal galloanserine birds were included in combination with sparse taxon sampling. Our phylogenetic results suggest that the earliest neornithines were heavy-bodied, ground-dwelling, nonmarine birds. This inference, coupled with a fossil bias toward marine environments, provides a possible explanation for the large gap in the early fossil record of birds. 相似文献
74.
Hao Z Fayolle L van Tuinen D Chatagnier O Li X Gianinazzi S Gianinazzi-Pearson V 《Journal of experimental botany》2012,63(10):3657-3672
The ectoparasitic dagger nematode (Xiphinema index), vector of Grapevine fanleaf virus (GFLV), provokes gall formation and can cause severe damage to the root system of grapevines. Mycorrhiza formation by Glomus (syn. Rhizophagus) intraradices BEG141 reduced both gall formation on roots of the grapevine rootstock SO4 (Vitis berlandieri×V. riparia) and nematode number in the surrounding soil. Suppressive effects increased with time and were greater when the nematode was post-inoculated rather than co-inoculated with the arbuscular mycorrhizal (AM) fungus. Using a split-root system, decreased X. index development was shown in mycorrhizal and non-mycorrhizal parts of mycorrhizal root systems, indicating that both local and systemic induced bioprotection mechanisms were active against the ectoparasitic nematode. Expression analyses of ESTs (expressed sequence tags) generated in an SSH (subtractive suppressive hybridization) library, representing plant genes up-regulated during mycorrhiza-induced control of X. index, and of described grapevine defence genes showed activation of chitinase 1b, pathogenesis-related 10, glutathione S-transferase, stilbene synthase 1, 5-enolpyruvyl shikimate-3-phosphate synthase, and a heat shock proein 70-interacting protein in association with the observed local and/or systemic induced bioprotection against the nematode. Overall, the data suggest priming of grapevine defence responses by the AM fungus and transmission of a plant-mediated signal to non-mycorrhizal tissues. Grapevine gene responses during AM-induced local and systemic bioprotection against X. index point to biological processes that are related either to direct effects on the nematode or to protection against nematode-imposed stress to maintain root tissue integrity. 相似文献
75.
Elena Kuznetsova Pascale M. A. Seddas-Dozolme Christine Arnould Marie Tollot Diederik van Tuinen Alexey Borisov Silvio Gianinazzi Vivienne Gianinazzi-Pearson 《Mycorrhiza》2010,20(6):427-443
The arbuscular mycorrhiza association results from a successful interaction between genomes of the plant and fungal symbiotic
partners. In this study, we analyzed the effect of inactivation of late-stage symbiosis-related pea genes on symbiosis-associated
fungal and plant molecular responses in order to gain insight into their role in the functional mycorrhizal association. The
expression of a subset of ten fungal and eight plant genes, previously reported to be activated during mycorrhiza development,
was compared in Glomus intraradices-inoculated wild-type and isogenic genotypes of pea mutated for the PsSym36, PsSym33, and PsSym40 genes where arbuscule formation is inhibited or fungal turnover modulated, respectively. Microdissection was used to corroborate
arbuscule-related fungal gene expression. Molecular responses varied between pea genotypes and with fungal development. Most
of the fungal genes were downregulated when arbuscule formation was defective, and several were upregulated with more rapid
fungal development. Some of the plant genes were also affected by inactivation of the PsSym36, PsSym33, and PsSym40 loci, but in a more time-dependent way during root colonization by G. intraradices. Results indicate a role of the late-stage symbiosis-related pea genes not only in mycorrhiza development but also in the
symbiotic functioning of arbuscule-containing cells. 相似文献
76.
Plant-driven selection of microbes 总被引:2,自引:0,他引:2
Anton Hartmann Michael Schmid Diederik van Tuinen Gabriele Berg 《Plant and Soil》2009,321(1-2):235-257
77.
Convergence and divergence in the evolution of aquatic birds. 总被引:8,自引:0,他引:8
M Van Tuinen D B Butvill J A Kirsch S B Hedges 《Proceedings. Biological sciences / The Royal Society》2001,268(1474):1345-1350
Aquatic birds exceed other terrestrial vertebrates in the diversity of their adaptations to aquatic niches. For many species this has created difficulty in understanding their evolutionary origin and, in particular, for the flamingos, hamerkop, shoebill and pelecaniforms. Here, new evidence from nuclear and mitochondrial DNA sequences and DNA-DNA hybridization data indicates extensive morphological convergence and divergence in aquatic birds. Among the unexpected findings is a grouping of flamingos and grebes, species which otherwise show no resemblance. These results suggest that the traditional characters used to unite certain aquatic groups, such as totipalmate feet, foot-propelled diving and long legs, evolved more than once and that organismal change in aquatic birds has proceeded at a faster pace than previously recognized. 相似文献
78.
Phylogeny and biogeography of ratite birds inferred from DNA sequences of the mitochondrial ribosomal genes 总被引:7,自引:2,他引:5
The origin of the flightless ratite birds of the southern continents has
been debated for over a century. Whether dispersal or vicariance
(continental breakup) best explains their origin depends largely on their
phylogenetic relationships. No consensus has been reached on this issue
despite many morphological and molecular studies. To address this question
further we sequenced a 2.8-kb region of mitochondrial DNA containing the
ribosomal genes in representative ratites and a tinamou. Phylogenetic
analyses indicate that Struthio (Africa) is basal and Rhea (South America)
clusters with living Australasian ratites. This phylogeny agrees with
transferrin and DNA hybridization studies but not with sequence analyses of
some protein-coding genes. These results also require reevaluation of the
phylogenetic position of the extinct moas of New Zealand. We propose a new
hypothesis for the origin of ratites that combines elements of dispersal
and vicariance.
相似文献
79.
P Van Tuinen A R Mootnick S C Kingswood D W Hale A T Kumamoto 《American journal of physical anthropology》1999,110(2):129-142
Karyotypic variation in five gibbon species of the subgenus Hylobates (2n = 44) was assessed in 63 animals, 23 of them wild born. Acquisition of key specimens of Hylobates agilis (agile gibbon), whose karyotype had been problematic due to unresolved structural polymorphisms, led to disclosure of a compound inversion/translocation polymorphism. A polymorphic region of chromosome 8 harboring two pericentric inversions, one nested within the other, was in turn bissected by one breakpoint of a reciprocal translocation. In double-inversion + translocation heterozygotes, the theoretical meiotic pairing configuration is a double inversion loop, with four arms of a translocation quadrivalent radiating from the loop. Electron-microscopic analysis of synaptonemal complex configurations consistently revealed translocation quadrivalents but no inversion loops. Rather, nonhomologous pairing was evident in the inverted region, a condition that should preclude crossing over and the subsequent production of duplication-deficiency gametes. This is corroborated by the existence of normal offspring of compound heterozygotes, indicating that fertility may not be reduced despite the topological complexity of this polymorphic system. The distribution of inversion and translocation morphs in these taxa suggests application of cytogenetics in identifying gibbon specimens and avoiding undesirable hybridization in captive breeding efforts. 相似文献
80.
Ruben Ortega Perez Diederik Van Tuinen Dieter Marmé Gilbert Turian 《Biochimica et Biophysica Acta (BBA)/General Subjects》1983,758(1):84-87
Cyclic nucleotide phosphodiesterase has been partially purified by calmodulin-Sepharose affinity chromatography from a soluble extract of Neurospora crassa. The phosphodiesterase activity remained bound to the affinity column even in the presence of 6 M urea and could only be eluted by calcium chelation. The enzyme exhibits cAMP and cGMP phosphodiesterase activities. Both activities can be enhanced by calmodulin in a Ca2+-dependent manner. Stimulation of cyclic nucleotide phosphodiesterase by calmodulin can be inhibited by calmodulin antagonists such as pimozide, trifluoperazine and chlorpromazine. 相似文献