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21.
22.
Saray A Kilic D Kaygusuz S Boyunaga H Ozlük O 《Plastic and reconstructive surgery》2004,114(5):1170-1178
Infection is a serious complication of breast augmentation and tissue expansion with inflatable devices. Several reports have shown that fungi may be able to survive, colonize, and even cause infection in saline-filled devices. The mechanism of how they penetrate, spread, and colonize inside the inflatable implants is not exactly understood. The authors assessed both the expander membrane and the port in terms of leakage and penetration of Candida albicans and Aspergillus niger in an in vitro model. Thirty saline-filled expanders connected to the injection port were placed in sterile containers filled with tryptic soy broth culture medium to simulate the clinical situation in phases I and II. Intact and multipunctured ports were used in the first and second phases of the study, respectively. Either the container or the implant was inoculated with one of these fungi, and six implants in containers without fungal inoculation served as controls. As a third phase, intraluminal survival of fungi was investigated in saline-filled containers (n = 12) in 21 days. The silicone membrane, with its intact connecting tube and port, was impermeable to these fungi, whereas both fungi were able to diffuse inside-out or outside-in through the punctured ports. C. albicans did not survive beyond 18 days in saline, whereas A. niger continued to multiply at day 21. Chemical analyses of the implant fluids revealed that the contents of the culture medium diffused into the implants in phases I and II. The data show that an intact silicone membrane is impermeable to fungi, and punctured ports allow translocation of fungi into the implants. Fungi can grow and reproduce in a saline-only environment, and their survival periods differ among the species. Furthermore, their survival may be enhanced by the influx of substances through the implant shell. 相似文献
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We report a prenatally diagnosed balanced de novo translocation t(6;7)(p25;q31). Physical examination of the baby born at term revealed only a posterior cleft palate. Laboratory examinations and radiologic investigations were found normal. Two years follow-up of the patient showed her mental and motor development was appropriate with her age. Our report is the first observation on balanced de novo translocation t(6;7)(p25;q31) and cleft palate. Association of this translocation and cleft palate has not been reported previously. 相似文献
25.
Akbas F Aydin M Cenani A 《Anthropologischer Anzeiger; Bericht über die biologisch-anthropologische Literatur》2003,61(3):257-260
We determined the AB0 blood group system with a PCR based technique termed APLP (Amplified Product Length Polymorphism) in the Turkish population. The method includes ten different allele specific primers and permits identification of the major AB0 genotypes and its suballeles (A1-A2-B-0A-02-0G-AG). The suballeles were amplified in a single tube reaction. We have determined AB0 phenotypes in 129 Turkish individuals. No significant deviation from the Hardy-Weinberg equilibrium was observed. 相似文献
26.
Kanber Y Demirbag NR Sam AD Aydin N 《The International journal of biological markers》2002,17(3):165-168
The aim of this study was to investigate the role of cathepsin D in colorectal cancer. For this purpose cathepsin D expression was evaluated by means of immunohistochemistry in stromal and tumor cells of 31 colorectal carcinomas and 29 adenomas. Cytoplasmic cathepsin D expression of tumor cells was present in 90.3% of the carcinoma cases and various degrees of stromal cell cathepsin D expression were present in all cases. In the adenomas, the epithelial cells and stromal cells expressed cathepsin D in 68.96% and 96.55% of cases, respectively. The staining intensity was always weaker in the adenomas. When the stromal and tumor cell cathepsin D expression in the adenocarcinoma and adenoma cases were compared, a statistically significant difference was observed in the staining of stromal cells. Furthermore, stromal cathepsin D expression in the adenocarcinomas was related to tumor stage when the carcinomas were divided into low and high stage. Cathepsin D expression in stromal cells may be an important indicator of poor prognosis in colorectal adenocarcinomas. 相似文献
27.
Işcan M Ada AO Coban T Kapucuoğlu N Aydin A Isimer A 《Biological trace element research》2002,89(2):177-190
When male rats were given a single dose of cadmium (Cd) (3.58 mg CdCl2·H2O/kg, ip) 72 hr prior to sacrifice, the testicular 7-ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST) activities toward the substrates 1-chloro-2,4-dinitrobenzene (CDNB), 1,2-dichloro-4-nitrobenzene (DCNB),
ethacrynic acid (EAA), 1,2-epoxy-3-(p-nitrophenoxy)-propane (EPNP), and cumene hydroperoxide (CHPx) decreased significantly as compared to controls. Cd also inhibited
reduced glutathione (GSH) level while increasing the lipid peroxidation (LP) level significantly. When the animals were given
a single dose of nickel (Ni) (59.5 mg NiCl2·6H2O/kg, ip) 16 hr prior to sacrifice, significant decreases were observed in EROD and GST activities toward CDNB, EAA, EPNP,
and CHPx, and GSH level. No significant alterations were noted in DCNB GST activity and LP level by Ni. For the combined treatment,
rats received the single dose of Ni 56 hr after the single dose of Cd and were killed 16 hr later. In these animals, lesser
depressions were observed on EROD activity and LP level than those of Cd alone. The combination of metals significantly inhibited
GST activities and GSH level but not to a greater degree than noted by Cd or Ni alone. Plasma testosterone levels of Cd-,
Ni-, and combination-treated rats decreased significantly compared to controls. The strongest depression was achieved by Cd
alone. Cd, both alone and in combination with Ni, increased the tissue Ni uptake significantly. Ni, however, did not produce
such an effect on the tissue uptake of Cd in either case. Cd treatment caused interstitial edema and coagulation necrosis
in seminiferous tubules and also caused fibrinoidal necrosis in vascular endothelium. Ni treatment did not produce any pathological
testicular alterations compared to controls. Combined treatment produced fewer pathological alterations (i.e., only interstitial
edema) than that of Cd treatment. These results reveal that the combination of Cd and Ni does not have a synergistic effect
on testicular xenobiotic metabolizing enzymes, and in contrast, Ni has an ameliorating effect on pathological disturbances
caused by Cd alone in the rat testis. 相似文献
28.
Baydar T Papp A Aydin A Nagymajtenyi L Schulz H Isimer A Sahin G 《Biological trace element research》2003,92(3):231-244
The present study was undertaken to examine possible aluminum (Al) accumulation in the brain of rats and to investigate whether
subchronic exposure to the metal leads to behavioral and neurophysiological changes in both treated and control groups. Each
of the groups consisted of 10 animals. Aluminum chloride (AlCl3) at a low (50 mg/kg/d) or high (200 mg/kg/d) dose was applied to male Wistar rats by gavage for 8 wk. Al-free water by gavage
was given to the control group throughout the experiment. Behavioral effects were evaluated by open-field (OF) motor activity
and by acoustic startle response (ASR). Electrophysiological examination was done by recording spontaneous activity and sensory-evoked
potentials from the visual, somatosensory, as well as auditory cortex. The Al content of each whole brain was determined by
electrothermal atomic absorption spectrophotometry. Subchronic Al exposure slightly caused some changes in the evoked potentials
and electrocorticograms and in the OF and ASR performance, but these results were not statistically significant. The brain
Al levels of the control and the low and high dose of Al-exposed groups were measured as 0.717±0.208 μg/g (wet weight), 0.963±0.491
μg/g (wet weight) and 1.816±1.157 μg/g (wet weight), respectively. 相似文献
29.
Copper is an essential trace element with various biological functions. Excess copper, however, is extremely toxic, leading
to many pathological conditions that are consistent with oxidative damage to membranes and molecules. Exposure to high levels
of copper results in various changes in the tissues. In liver, hypertrophy of hepatocytes, hepatitis, hepatocellular necrosis,
and hepatocellular death are the results. Lipid peroxidation causes dysfunction in the cell membrane, decreased fluidity,
inactivation of receptors and enzymes, and changes ion permeability. In this study, we aimed to determine the effect of copper
on oxidative and antioxidative substances in plasma and liver tissue in a rat model.
Sixteen male Sprague—Dawley rats were divided into two groups: Group 1 rats included control rats given tap water. Group 2
rats were given water containing copper in a dose of 100 μg/mL. All rats were sacrificed at 4 wk under ether anesthesia. Plasma
and liver superoxide dismutase (SOD) activities, plasma and liver MDA (malondialdehyde) levels, and liver glutathione (GSH)
levels were studied. Plasma and liver SOD activities were found to be higher in group 2 than those in group 1. Although plasma
MDA levels were higher in group 2, MDA levels in liver tissues were comparable. Liver tissue glutathione levels were lower
in group 2. It was concluded that although copper is needed in trace amounts, an excess amount is toxic for the organism.
It increases lipid peroxidation and depletes GSH reserves, which makes the organism more vulnerable to other oxidative challenges. 相似文献
30.
A number of studies have reported that oxidant stress reduces the activity of isolated Na(+)-K(+) ATPase and Ca(2+) ATPase which are known to affect the cell membrane integrity. The aim of the study is to determine whether the administration of lisinopril is able to protect the membrane-bound enzyme levels in isolated guinea pig hearts and also ascertain whether or not a relationship exists between oxygen free radicals and membrane bound Na(+)-K(+) ATPase and Ca(2+) ATPase. Forty guinea pig hearts were studied in an isolated Krebs-Henseleit solution-perfused Langendorff cardiac model. In all groups cardioplegic arrest was achieved by administering St. Thomas' Hospital cardioplegic solution (STHCS). Group 1 (control, n=10) received only STHCS. Group 2 (n=10) were arrested with lisinopril (l micromol l(-1)) added STHCS. Group 3 (n=10) were pretreated with oral lisinopril (0.2 mg kg(-1) twice a day) for 10 days and then arrested with STHCS. Group 4 were also pretreated with oral lisinopril (0.2 mg kg(-1) twice a day for 10 days), arrested with STHCS and reperfused with lisinopril added to Krebs-Henseleit solution (l micromol l(-1)). Hearts were subjected to normothermic global ischaemia for 90 min and then reperfused at 37 degrees C. Pretreatment and addition of lisinopril in the reperfusion buffer improved the levels of membrane-bound enzymes. When the treated groups were compared with control hearts, the best results were achieved in group 4. The Na(+)-K(+) and Ca(2+) ATPase levels increased from 466.38+/-5.99 to 560.12+/-18.02 and 884.69+/-9.13 to 1287.71+/-13.01 nmolPi mg(-1) protein h(-1) respectively (p<0.05). These results suggest that lisinopril protects the cell membrane integrity and lessens free radical-induced oxidant stress. 相似文献