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91.
Analysis of Vibrio seventh pandemic island II and novel genomic islands in relation to attachment sequences among a wide variety of Vibrio cholerae strains 下载免费PDF全文
Tuan Hai Nguyen Tho Duc Pham Naomi Higa Hanako Iwashita Taichiro Takemura Makoto Ohnishi Kouichi Morita Tetsu Yamashiro 《Microbiology and immunology》2018,62(3):150-157
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Karinna?Chouman Birgit?Korioth-Schmitz Markus?Sack J?rn?Engelbert?Schmitz Anh?Tuan?Pham Rainer?Fischer Stefan?Barth Torsten?KlockenbringEmail author Rolf?Fendel 《European cytokine network》2018,29(2):59-72
Interleukin-6 (IL-6) expression and secretion, induced by inflammatory processes, stimulate the acute phase response cascade. The overexpression of IL-6 contributes to a variety of inflammatory diseases, e.g. rheumatoid arthritis, Castleman’s disease, multiple myeloma, and prostate cancer. Screening for high amounts of IL-6 in the patients’ blood serum can be crucial for an adequate treatment. In this study, five novel murine monoclonal antibodies (mAbs) reactive to human IL-6 were generated. The mAbs were characterized for potential diagnostic purposes and recombinant antibodies were derived thereof. Initial epitope mapping using a combination of blocking experiments and Hyper-IL-6, a fusion protein consisting of IL-6 and the soluble IL-6 receptor revealed distinct but overlapping binding sites. At least one of the mAbs was found to interact with the region of IL-6/ IL-R complex formation. Three mAbs were applied successfully in intracellular staining by flow cytometry, whereas one of the mAbs showed comparable binding as a reference reagent. Furthermore, the mAbs were tested for applications in various immunological assays such as ELISA, Western blot and surface plasmon resonance spectroscopy (SPR), using IL-6 from commercial sources as well as in-house produced protein (IL-6_IME). The limit of detection was determined by sandwich ELISA (0.5 ng/mL, SD ±0.005). Our results also demonstrated that the recombinant IL- 6 produced was functional and correctly folded. These findings support the use of the generated mAb clones as promising candidates for application in various immunological assays for diagnostic and scientific purposes. 相似文献
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Rmg8 and Rmg7, wheat genes for resistance to the wheat blast fungus,recognize the same avirulence gene AVR‐Rmg8 下载免费PDF全文
Vu Lan Anh Yoshihiro Inoue Soichiro Asuke Trinh Thi Phuong Vy Nguyen Tuan Anh Shizhen Wang Izumi Chuma Yukio Tosa 《Molecular Plant Pathology》2018,19(5):1252-1256
Rmg8 and Rmg7 are genes for resistance to the wheat blast fungus (Pyricularia oryzae), located on chromosome 2B in hexaploid wheat and chromosome 2A in tetraploid wheat, respectively. AVR‐Rmg8, an avirulence gene corresponding to Rmg8, was isolated from a wheat blast isolate through a map‐based strategy. The cloned fragment encoded a small protein containing a putative signal peptide. AVR‐Rmg8 was recognized not only by Rmg8, but also by Rmg7, suggesting that these two resistance genes are equivalent to a single gene from the viewpoint of resistance breeding. 相似文献
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Guiomar Rotllant Tuan Viet Nguyen David Hurwood Valerio Sbragaglia Tomer Ventura Joan B. Company Silvia Joly Abigail Elizur Peter B. Mather 《Hydrobiologia》2018,808(1):137-152
In marine ecosystems, the most significant migration observed in terms of biomass distribution is the one connected with the vertical movements in the water column. In the present study, the vertical profiles of the mesopelagic shrimps Gennadas elegans, Eusergestes arcticus, Sergia robusta, and the epipelagic Parasergestes vigilax in the Balearic Sea (western Mediterranean), during the stratified (summer) and non-stratified (autumn) hydrographic conditions, were investigated through their ontogeny, from the larval to adult stages. The mesopelagic adults were observed to move down to the deeper layers during the night more than during the daylight hours. Most larvae aggregated within the limits of the upper water column. The P. vigilax larvae were collected only during the stratified period. The first two larval stages vertical distribution indicates that the mesopelagic crustacean spawning could occur at greater depths. During the non-stratified period, the larvae of the mesopelagic species tended to remain at about 500 m depth at night, rising towards the upper layers at sunrise. Vertical patterns are discussed, as strategies associated with predator–prey trade-offs. To our knowledge, the present study is the first such attempt to jointly analyze the vertical migrations of the developmental stages of the pelagic shrimps in the Mediterranean Sea. 相似文献
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Bai Songling Tuan Pham Anh Tatsuki Miho Yaegaki Hideaki Ohmiya Akemi Yamamizo Chihiro Moriguchi Takaya 《Plant Molecular Biology Reporter》2016,34(1):257-264
Plant Molecular Biology Reporter - Transgenic approach is an excellent way for the clarification of gene function, but it is generally difficult to create transgenic plants for most of the fruit... 相似文献
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