全文获取类型
收费全文 | 117篇 |
免费 | 4篇 |
出版年
2020年 | 1篇 |
2018年 | 5篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 2篇 |
2013年 | 3篇 |
2012年 | 5篇 |
2011年 | 1篇 |
2010年 | 1篇 |
2009年 | 3篇 |
2008年 | 2篇 |
2007年 | 5篇 |
2006年 | 5篇 |
2005年 | 2篇 |
2004年 | 3篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2001年 | 5篇 |
2000年 | 5篇 |
1999年 | 3篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 1篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1976年 | 1篇 |
1975年 | 5篇 |
1973年 | 5篇 |
1972年 | 4篇 |
1971年 | 1篇 |
1970年 | 5篇 |
1969年 | 9篇 |
1968年 | 4篇 |
1966年 | 3篇 |
1965年 | 1篇 |
排序方式: 共有121条查询结果,搜索用时 15 毫秒
11.
12.
A.N. Reshetilov A.B. Medvinsky T.P. Eliseeva V.Yu. Shakhbazian M.A. Tsyganov A.M. Boronin G.R. Ivanitsky 《FEMS microbiology letters》1992,94(1-2):59-62
A method of pH distribution measurements in agar nutrient media containing expanding bacterial populations is described. It is based on measuring pH microsamples taken at different points of the media. The sample volume was 10 microliters. A pH sensitive field effect transistor was used as a measuring electrode. Acidification was found to occur in glucose media, while alkalization occurred in the media containing peptone. 相似文献
13.
The results of numerical experiments with mathematical models of excitable systems with cross-diffusion are presented. It was shown that the refractoriness in such systems may be negative. The effects of negative refractoriness on the propagation and interaction of waves are demonstrated. 相似文献
14.
15.
16.
17.
18.
19.
Identification of DNA amplification fingerprinting (DAF) markers close to the symbiosis-ineffective sym31 mutation of pea (Pisum sativum L.) 总被引:1,自引:0,他引:1
A. E. Men A. Y. Borisov S. M. Rozov K. V. Ushakov V. E. Tsyganov I. A. Tikhonovich P. M. Gresshoff 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):929-936
We demonstrate efficient genome mapping through a combination of bulked segregant analysis (BSA) with DNA amplification fingerprinting
(DAF). Two sets of 64 octamer DAF primers, along with two PCR programs of low- and high-annealing temperatures (30°C and 55°C,
respectively), appeared to be enough to locate molecular markers within 2–5 cM of a gene of interest. This approach allowed
the rapid identification of four BSA markers linked to the pea (Pisum sativum L.) Sym31 gene, which is responsible for bacteroid and symbiosome differentiation. Three of these markers are shown to be tightly linked
to the sym31 mutation. Two markers flanking the Sym31 gene, A21-310 and B1-277, cover a 4–5 cM interval of pea linkage group 3. Both markers were converted to sequence-characterized
amplified regions (SCARs). The flanking markers may be potential tools for marker-assisted selection or for positional cloning
of the Sym31 gene.
Received: 2 July 1998 / Accepted: 8 October 1998 相似文献
20.
A. V. Tsyganova V. E. Tsyganov K. C. Findlay A. Y. Borisov I. A. Tikhonovich N. J. Brewin 《Cell and Tissue Biology》2009,3(1):93-102
The interface between the host cell and the microsymbiont is an important zone for development and differentiation during
consecutive stages of Rhizobium-legume symbiosis. Legume root nodule extensins, otherwise known as arabinogalactan protein-extensins
(AGPEs) are abundant components of infection thread matrix. We have characterized the origin and distribution of these glycoproteins
at the symbiotic interface of root nodules of symbiotically defective mutants of pea (Pisum sativum L.) by using immunogold localization with MAC265 an anti-AGPE monoclonal antibody. For mutants with defective growth of infection
threads, the AGPE epitope was abundant in the extracellular matrix surrounding infected host cells in the central infected
tissue of the nodule, as well as in the lumen of Rhizobiuminduced infection threads. This seems to indicate a mistargeting
of AGPE as a consequence of abnormal growth of the infection threads. Furthermore, mutants in the gene sym33 showed reduced labeling with MAC265 and, in some infection threads and droplets, the label was completely absent, a phenomenon
that is not observed in wild-type nodules. This suggests an alteration in the composition of the infection thread matrix for
sym33 mutants, which may be correlated to the absence of endocytosis of rhizobia into the host cytoplasm. 相似文献