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961.
The cysteine-rich peptide hepcidin is known to be an antimicrobial peptide and iron transport regulator that has been found in both fish and mammals. Recently, we found two different types (designated Hep-JF1 and Hep-JF2) of hepcidin cDNA in the Japanese flounder, Paralichthys olivaceus, by expressed sequence tag analysis. The identity of amino acid sequences between Hep-JF1 and Hep-JF2 was 51%. The Hep-JF1 and Hep-JF2 genes both consist of three exons and two introns, and both exist as single copies in the genome. The predicted mature regions of Hep-JF1 and Hep-JF2 have six and eight Cys residues, respectively. The first Cys residue of Hep-JF1 was deleted and the second was replaced with Gly. The number and positions of Cys residues in Hep-JF2 are the same as they are in human Hep. Hep-JF1 is specifically expressed in liver while the expression of Hep-JF2 was detected from gill, liver, heart, kidney, peripheral blood leucocytes, spleen and stomach. Gene expression of Hep-JF1 in liver decreased during experimental iron (iron-dextran) overload. Expression of Hep-JF1 in liver was decreased by injecting fish with iron-dextran and increased by injecting lipopolysaccharide. Iron overload did not significantly affect expression of Hep-JF2 in liver but it did increase expression of Hep-JF2 in kidney. Lipopolysaccharide injection increased expression of Hep-JF2 in both liver and kidney. In liver, some cells expressed both Hep-JF1 and Hep-JF2 while some other cells expressed just one of them. Synthesized Hep-JF2 peptide showed antimicrobial activity, while synthesized Hep-JF1 peptide did not against several bacteria including fish-pathogenic bacteria used in this study. 相似文献
962.
Tsuyoshi?YoshidaEmail author Kazuhiro?Tanaka 《Landscape and Ecological Engineering》2005,1(2):201-206
Preservation of ecological diversity is a guiding principle of landscape management and planning. Although diversity is often quantified with measurable indices, common approaches used to measure diversity, for example the Shannon–Weaver index, are not adequate for many landscape studies, because they are affected by scale and sampling efforts. A robust index for measurement of landscape diversity should be able to quantify distinctive components of a landscape mosaic. This paper provides a new diversity index for landscape studies that improves the measurement of diversity at landscape level. The key features of the method are inclusion of an implication of physical moment and quantification of diversity by placing greater weight on the structure and composition of a patch. We have named the new index the land-use diversity index, or LUDI, and have concluded that the LUDI is the preferred measure of diversity at landscape level. 相似文献
963.
964.
Hyeong‐Woo Kim Satoshi Ohnishi Minoru Fujii Tsuyoshi Fujita Hung‐Suck Park 《Journal of Industrial Ecology》2018,22(2):275-287
Industrial symbiosis (IS) exchanges have been recognized to reduce greenhouse gas (GHG) emission, though methods for quantification of GHG emissions in IS exchanges are varied, and no standardized methods are available. This article proposes a practical approach to quantify total and allocated GHG emissions from IS exchanges by integrating the GHG protocol and life cycle assessment. The proposed method expands the system boundaries to include all IS companies, and the functional flow is set to be the sum of the main products. The total impact of a company is allocated to the main product. Three by‐product impact allocation methods of cutoff, avoidance, and 50/50 are proposed, and the total and distributed impacts of the IS systems in an industrial park are theoretically derived. The proposed method was tested to quantify GHG reduction in a real IS exchange developed between Korea Zinc (a zinc smelter) and Hankook Paper (a paper mill company) in the Ulsan Eco‐Industrial Park initiative. The total reduction of GHG emissions in this IS exchange, 60,522 tonnes of carbon dioxide per year, was the same in the GHG protocol, whereas GHG distribution between two companies depended on the allocation method. Given that the reduction of GHG emissions from IS exchanges is the product of the collaboration of giving companies and receiving companies, the 50/50 allocation method is best from an equivalent‐responsibility and benefit‐sharing perspective. However, this study suggests a more practical implementation approach based on a flexible and negotiable method of allocating the total GHG reduction between stakeholders. 相似文献
965.
Nobuharu Inaba Satoshi Nagai Tomoko Sakami Tsuyoshi Watanabe Kiyo Araki Shinji Kawasaki 《Bioremediation Journal》2018,22(3-4):112-125
AbstractTemporal changes of algicidal and growth-inhibiting bacteria on the fish-killing raphidophyte flagellate, Chattonella antiqua, at an eelgrass (Zostera marina) bed in southern Ariake Sea, Japan in 2011 was investigated. The maximum value (5.1?×?107 CFU g?1 wet leaf) of algicidal bacteria (AB) was detected from a biofilm formed on Z. marina on August 1 when AB in the adjacent seawater had also peaked (1.2?×?104 CFU mL?1). Two causative bacteria isolated from the biofilm and seawater on August 1 were both identified to be of the genus Alteromonas (γ-proteobacteria). AB and growth-inhibiting bacteria (GIB) were present from the beginning of sampling (May 20) to August 26, fluctuating between 8.6?×?102 and 1.2?×?104, 1.2?×?103 and 9.3?×?103 CFU mL?1, respectively. The highest phytoplankton density observed was 6423 cells mL?1 on September 29 and was comprised of centric diatoms such as Chaetoceros, Skeletonema, and Thalassiosira and coincided with the absence of AB and GIB where the decline of Z. marina was also observed. These findings provide a new ecological insight on AB and GIB associated with Z. marina beds, indicating eelgrass beds have the important role as the nursery of those bacteria that can be utilized as mitigation measures of harmful algal blooms (HABs) in the future. 相似文献
966.
Tetsuya Toba Ryosuke Suzuki Junko Futamura-Takahashi Yoshito Kawamoto Shigeki Tamura Mariko Kuroda Yoshiari Shimmyo Michinori Kadokura Kazumichi Goto Teruyoshi Inoue Tsuyoshi Muto Hirokazu Annoura 《Bioorganic & medicinal chemistry letters》2018,28(14):2528-2532
A series of compounds was discovered that induce the production of VGF mRNA in SH-SY5Y cells and exhibit cytoprotection under tunicamycin induced endoplasmic reticulum (ER) stress. The aminophenol ring and linker chain of the template SUN N8075 (1) was modified to yield compounds with higher efficacy and lower propensity for adverse effects. 相似文献
967.
Junko Futamura-Takahashi Taisaku Tanaka Hajime Sugawara Shinzo Iwashita Seiichi Imajo Yoshiaki Oyama Tsuyoshi Muto 《Bioorganic & medicinal chemistry letters》2018,28(2):188-192
Based on insight from the X-ray crystal structure of human chymase in complex with compound 1, a lactam carbonyl of the diazepane core was exchanged with O-substituted oxyimino group, leading to amidoxime derivatives. This modification resulted in highly potent chymase inhibitors, such as O-phenylamidoxime 5f. X-ray crystal structure analysis indicated that compound 5f induced movement of the Leu99 and Tyr94 side chains at the S2 site, and the increase in inhibitory activity of O-phenyl amidoxime derivatives suggested that the O-phenyl moiety interacted with the Tyr94 residue. Surface plasmon resonance experiments showed that compound 5f had slower association and dissociation kinetics and the calculated residence time of compound 5f to human chymase was extended compared to that of amide compound 1. 相似文献
968.
Clinical utility of ICD‐11 diagnostic guidelines for high‐burden mental disorders: results from mental health settings in 13 countries 下载免费PDF全文
Geoffrey M. Reed Jared W. Keeley Tahilia J. Rebello Michael B. First Oye Gureje José Luis Ayuso‐Mateos Shigenobu Kanba Brigitte Khoury Cary S. Kogan Valery N. Krasnov Mario Maj Jair de Jesus Mari Pratap Sharan Dan J. Stein Min Zhao Tsuyoshi Akiyama Howard F. Andrews Elson Asevedo Majda Cheour Tecelli Domínguez‐Martínez Joseph El‐Khoury Andrea Fiorillo Jean Grenier Nitin Gupta Lola Kola Maya Kulygina Itziar Leal‐Leturia Mario Luciano Bulumko Lusu J. Nicolás I. Martínez‐López Chihiro Matsumoto Mayokun Odunleye Lucky Umukoro Onofa Sabrina Paterniti Shivani Purnima Rebeca Robles Manoj K. Sahu Goodman Sibeko Na Zhong Wolfgang Gaebel Anne M. Lovell Toshimasa Maruta Kathleen M. Pike Michael C. Roberts María Elena Medina‐Mora 《World psychiatry》2018,17(3):306-315
In this paper we report the clinical utility of the diagnostic guidelines for ICD‐11 mental, behavioural and neurodevelopmental disorders as assessed by 339 clinicians in 1,806 patients in 28 mental health settings in 13 countries. Clinician raters applied the guidelines for schizophrenia and other primary psychotic disorders, mood disorders (depressive and bipolar disorders), anxiety and fear‐related disorders, and disorders specifically associated with stress. Clinician ratings of the clinical utility of the proposed ICD‐11 diagnostic guidelines were very positive overall. The guidelines were perceived as easy to use, corresponding accurately to patients’ presentations (i.e., goodness of fit), clear and understandable, providing an appropriate level of detail, taking about the same or less time than clinicians’ usual practice, and providing useful guidance about distinguishing disorder from normality and from other disorders. Clinicians evaluated the guidelines as less useful for treatment selection and assessing prognosis than for communicating with other health professionals, though the former ratings were still positive overall. Field studies that assess perceived clinical utility of the proposed ICD‐11 diagnostic guidelines among their intended users have very important implications. Classification is the interface between health encounters and health information; if clinicians do not find that a new diagnostic system provides clinically useful information, they are unlikely to apply it consistently and faithfully. This would have a major impact on the validity of aggregated health encounter data used for health policy and decision making. Overall, the results of this study provide considerable reason to be optimistic about the perceived clinical utility of the ICD‐11 among global clinicians. 相似文献
969.
Hiroyuki Saito Yoshihiro Takahashi Seizaburo Harata Keiko Tanaka Tsuyoshi Sano Tsunehisa Suto Akio Yamada Shudo Yamazaki Morihiro Morita 《Microbiology and immunology》1996,40(4):271-275
In 1993, mumps with a high incidence of aseptic meningitis became prevalent in Akita prefecture, Japan. Three mumps virus isolates obtained from the nonvaccine-associated cases lacked the BamHI restriction cleavage site of the P gene, like the Urabe strain (Yamada, A. et al, Vaccine 8: 553-557). However, four additional nucleotide substitutions were found in the determined region of 157 bp. Fourteen of 19 cases from which mumps virus showing the Urabe-like RFLP profile was detected were complicated with symptomatic meningitis, whereas there were only four cases of meningitis among 23 individuals infected with the wild type showing no Urabe-like RFLP profile (non-“Urabe-like” wild-type). The incidence of meningitis was over 70% among patients infected with the “Urabe-like” wild-type virus. The “Urabe-like” wild-type disappeared after February 1994 in the epidemic area and was replaced by the non-“Urabe-like” wild-type. Patients infected with the “Urabe-like” wild-type lived in a closed colony, in which there were two instances of transmission between siblings. Thus this outbreak was transient and narrowly localized. 相似文献
970.
Takahiko Tanigawa Jun Suzuki Tsuyoshi Ueta Tetsuo Katsumoto Yoshinori Tanaka 《Microbiology and immunology》1996,40(1):81-84
We have surveyed the sensitivity of cells in macrophage lineage to Streptolysin-O (SLO). SLO had cytotoxic activity on immature myeloid cell lines such as M1 and WEHI-3BD+. SLO was toxic to the cells after a 2-hr incubation. However, mature macrophage cell lines such as A640-BB-2, J774, and P388D1 were not sensitive to the same dose of SLO. After M1 cells were treated with leukemia inhibitory factor (LIF), a differentiation-inducer to macrophage, these cells became insensitive to SLO in one day. This cytotoxic action of SLO was inhibited by pretreatment with anti-Streptolysin-O antibody or cholesterol. These results indicate that SLO has different effects on macrophage lineage. 相似文献