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91.
In exponentially growing cells of Synechococcus sp. 6301, over 95% of the phycobiliproteins are located in phycobilisomes, and the remainder is present in the form of low molecular weight aggregates. In addition to the subunits of the phycobiliproteins (C-phycocyanin, allophycocyanin, allophycocyanin B), the phycobilisomes of this unicellular cyanobacterium contain five non-pigmented polypeptides. During the initial phase of starvation (24 h after removal of combined nitrogen from the growth medium), the phycobiliproteins in the low molecular weight fraction largely disappeared. Phycocyanin was lost more rapidly from this fraction than allophycocyanin. Simultaneous changes in the phycobilisome were (1) a decrease in sedimentation coefficient, (2) a decrease in phycocyanin: allophycocyanin ratio, (3) a shift in the fluorescence emission maximum from 673 to 676 nm, and (4) a selective complete loss of a 30,000 dalton non-pigmented polypeptide. Upon extensive nitrogen starvation (72 h), the intracellular level of phycocyanin decreased by over 30-fold. These results indicate that in the early stage of nitrogen starvation, the free phycobiliproteins of the cell are degraded, as well as a significant proportion of the phycocyanin from the periphery of the phycobilisome. However, the structures partially depleted of phycocyanin still function efficiently in energy transfer. On extended starvation, total degradation of residual phycobilisomes takes place, possibly in conjunction with the detachment of these structures from the thylakoids.None of the effects of the absence of combined nitrogen were seen when cells were starved in the presence of chloramphenicol, or in a methionine auxotroph starved for methionine.Abbreviations Used NaK-PO4
NaH2PO4 titrated with K2HPO4 to a given pH
- SDS
sodium dodecyl sulfate
- Tris
Tris(hydroxymethyl)aminomethane 相似文献
92.
Hajime Tokuda Tsutomu Unemoto 《Biochemical and biophysical research communications》1981,102(1):265-271
The membrane potential generated at pH 8.5 by K+-depleted and Na+-loaded is not collapsed by proton conductors which, instead, induce the accumulation of protons in equilibrium with the membrane potential. The generation of such a membrane potential and the accumulation of protons are specific to Na+-loaded cells at alkaline pH and are dependent on respiration. Extrusion of Na+ at pH 8.5 occurs in the presence of proton conductors unless respiration is inhibited while it is abolished by proton conductors at acidic pH. The uptake of α-aminoisobutyric acid, which is driven by the Na+-electrochemical gradient, is observed even in the presence of proton conductors at pH 8.5 but not at acidic pH. We conclude that a respiration-dependent primary electrogenic Na+ extrusion system is functioning at alkaline pH to generate the proton conductor-insensitive membrane potential and Na+ chemical gradient. 相似文献
93.
Molecular and enzymatic properties of "cytochrome aa3"-type terminal oxidase derived from Nitrobacter agilis 总被引:5,自引:0,他引:5
Cytochrome c oxidase (cytochrome aa3-type) [EC 1.9.3.1] was purified from Nitrobacter agilis to an electrophoretically homogeneous state and some of its properties were studied. The enzyme showed absorption peaks at 422, 598, and 840 nm in the oxidized form, and at 442 and 606 nm in the reduced form. The CO compound of the reduced enzyme showed peaks at 436 and 604 nm, and the latter peak had a shoulder at 599 nm. The enzyme possessed 1 mol of heme a and 1.6 g-atom of copper per 41,000 g, and was composed of two kinds of subunits of 51,000 and 31,000 daltons. These results show that the structurally minimal unit of the enzyme molecule is composed of one molecule each of the two subunits and contains 2 molecules of heme a and 2-3 atoms of copper. the enzyme rapidly oxidized ferrocytochromes c of several eukaryotes as well as N. agilis ferrocytochrome c-552. The reactions catalyzed by the enzyme were strongly inhibited by KCN. The reduction product of oxygen catalyzed by the enzyme was concluded to be water on the basis of the ratio of ferrocytochrome c oxidized to molecular oxygen consumed. 相似文献
94.
A thiosulfate-oxidizing enzyme was partially purified fromChromatium vinosum, and some of its properties were studied. The enzyme rapidly reducede HiPIP (high-potential nonheme iron protein) in the
presence of thiosulfate. Cytochromesc of yeast and tuna and ferricyanide also acted well as electron acceptors for the enzyme; horse cytochromec was a poor electron acceptor. Cytochromec-552, cytochromec′, and cytochromec-553 did not act as electron acceptors. The enzyme was inhibited by cyanide and sulfite. On the basis of the stoichiometry
in reduction of ferricyanide catalyzed by the enzyme in the presence of thiosulfate, the oxidized product of thiosulfate was
inferred to be tetrathionate. 相似文献
95.
A New technique for the rapid measurement of ultrasonic absorption with a sampling interval of 5 msec has been developed and applied to the kinetic study of denaturation and subsequant redution of hen egg-white lysozyme. The lysozyme is denatured by guanidine hydrochloride (GuHCl) orLiBr, and afetr denaturation by GuHCl, its disulfide bonds are reduced by dithiothreitol (DTT). The ultrasonic adsorption coefficient at 9 MHz increases with denaturation but decreases with reduction. The rate constant of denaturation by GuHCl obtained from the rime variance of ultasonic agrees well with that from uv absorption and optical rotation. The time variance if absorption after GuHCl and Dtt have been simultaneously added exhibits two rate constants. Analysis of the constants as functions of regeant concentrations indicates that the intermediates state between native and reduced states is not necessarily the completely denatured state but depends on the concentartions of GuHCl and DTT. 相似文献
96.
97.
3′,4′-Dideoxykanamycin B, the kanamycin B derivative that is active against resistant bacteria, was prepared from kanamycin B viaN-tosylation, 3′,4′-O-sulphonylation, 3′,4′-unsaturation, and hydrogenation. The unsaturated intermediate was obtained from the 3′,4′-di-O-sulphonyl derivatives by the action of sodium iodide in N,N-dimethylformamide; if zinc dust was added in this reaction, aziridine derivatives were formed, Removal of the tosyl group was successfully performed by using sodium in ammonia-ethylamine. 相似文献
98.
A deoxyribonuclease, which requires nucleoside triphosphate for reaction, has been purified about 150-fold from extracts of Bacillus laterosporus. Potassium phosphate and ethylene glycol stabilize the purified enzyme. The enzyme degrades double-stranded DNA about 100 times faster than heat-denatured DNA in the presence of nucleoside triphosphate. Double-stranded DNA is not degraded to any measurable extent in the absence of ATP, but the enzyme exhibits activity toward denatured DNA in the absence of nucleoside triphosphate, and this activity seems to be an intrinsic property of this enzyme protein. The optimum pH is 8.5 and the maximum activity is obtained in the copresence of Mg2+ (8.0 X 10(-3)M) and Mn2+ (7.0 X 10(-5)M). ATP and dATP are most effective and nucleoside di- or monophosphates are ineffective. ATP is converted to ADP and inorganic phosphate during the reaction and the ratio of the amount of ATP cleaved to that of hydrolyzed phosphodiester bonds of DNA is about 3:1. An inhibitor of the enzyme was observed in bacterial extracts prepared by sonic disruption; the inhibitory substance is produced in the bacteria in the later stages of cell growth. Preliminary results show that the inhibitor emerged near the void volume of a Sephadex G-200 column, and was relatively heat-stable, RNase-resistant, and DNase-sensitive. 相似文献
99.
T Yamanaka 《Journal of biochemistry》1976,79(3):655-660
The subunits of cytochrome c-553 (Chlorobium thiosulfatophilum) were studied. The cytochrome is split into a cytochrome moiety and a flavoprotein moiety by treatment with 2% trichloroacetic acid. The molecular weights of the cytochrome and flavoprotein moieties are 11,000 and 47,000, respectively. The cytochrome moiety seems to have only one cysteine residue in the molecule, although its heme appears to be quite similar to the usual heme c. The flavoprotein moiety shows absorption peaks at 350 and 452nm and is insoluble at neutral pH. When the two moieties are mixed at alkaline pH, and the pH of the mixture is then brought to neutral, the flavoprotein moiety remains soluble. However, the preparation thus obtained is different from the original cytochrome c-553. 相似文献
100.
The antibiotic cordycepin (3'-deoxyadenosine), a known specificinhibitor of nucleic acid synthesis and polyadenylation of RNA,remarkably retarded decrease in the chlorophyll and proteincontents of senescing tobacco leaf disks. The effectivenessof cordycepin, at its optimum concentration (ca. 4 x 105M), was 53% as effective as a cytokinin, benzyladenine at 106M. Adenosine and 2'-deoxyadenosine had no anti-senescence action. (Received May 29, 1975; ) 相似文献