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Ichiro Kimura Yae Gotoh Eijiro Ozawa 《In vitro cellular & developmental biology. Plant》1989,25(3):236-242
Summary A mitogenic factor which promotes quail myoblast proliferation has been purified some 105-fold from chick embryo extract by a combination of cation-exchange chromatography and heparin-affinity chromatography. The
factor is eluted from heparin-Sepharose with 2M NaCl and is a single-chain polypeptide with an apparent molecular weight of 15000 to 17000. It is active at subnanogram level
in triggering the proliferation and thereby delaying temporarily fusion of myoblasts. It also stimulates the proliferation
of quail fibroblasts in a similar effective concentration range. For both myoblasts and fibroblasts the dose-response to the
factor is quantitatively and qualitatively comparable with that of bovine pituitary fibroblast growth factor. These observations
strongly suggest that the factor very probably corresponds to chicken fibroblast growth factor or to a closely related molecule(s)
and that it is possibly involved in the regulation of myogenesis.
This work was partly supported by a grant from the National Center of Neurology and Psychiatry (NCNP grant 86-01) of the Ministry
of Health and Welfare, Japan. 相似文献
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Effects on ventilatory responses to progressive isocapnic hypoxia of a synthetic potent progestin, chlormadinone acetate (CMA), were determined in the halothane-anesthetized male rat. Ventilation during the breathing of hyperoxic gas was largely unaffected by treatment with CMA when carotid chemoreceptor afferents were kept intact. The sensitivity to hypoxia evaluated by hyperbolic regression analysis of the response curve did not differ between the control and CMA groups. The reduction of ventilation after bilateral section of the carotid sinus nerve (CSN) in hyperoxia was less severe in CMA-treated than in untreated animals. Furthermore, the CMA-treated rats showed a larger increase in ventilation during the hypoxia test and a lower PO2 break point for ventilatory depression. Inhibition of hypoxic ventilatory depression by CMA persisted even after the denervation of CSN. We conclude that exogenous progestin likely protects regulatory mechanism(s) for respiration against hypoxic depression through a stimulating action independent of carotid chemoreceptor afferents and without a change in the sensitivity of the ventilatory response to hypoxia. 相似文献
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The studies for production of saikosaponins by tissue culture of Bupleurum falcatum L. were carried out to produce saikosaponins with several kinds of media and plant hormones. Among the media and plant hormones studied, Gamborg's B-5 [23] medium containing 0.5 ppm kinetin (k) and 1.0 ppm 3-indolebutyric acid (IBA) was the most effective medium and hormone for production of saikosaponins. The highest content of saikosaponin-d in the dried cells was 0.26%, which was similar to a concentration of Bupleuri Radix.Abbreviations
MS
medium used by Murashige and Skoog [22]
-
G
medium used by Gamborg (B 5) [23]
-
W
medium used by White [24]
-
NN
medium used by Nitsch and Nitsch [25]
-
k
Kinetin
-
BAP
6-Benzylaminopurine
-
2,4 D
2,4-Dichlorophenoxyacetic acid
-
NAA
-Naphtylacetic acid
-
IBA
3-Indolebutyric acid
-
IAA
3-Indoleacetic acid
-
ssd
saikosaponin-d
-
PM
Production medium
- dw
Dry weight 相似文献