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701.
K. Nonaka E. Kondratskaya M. Maeda T. Yamaga N. Murayama M. C. Shin N. Akaike 《Neurophysiology》2009,41(4):250-257
The action of ginkgolide B (GB), a powerful compound of Ginkgo biloba extract, on glycine-mediated spontaneous currents in rat spinal sacral dorsal commissural nucleus (SDCN) neurons was examined.
IPSCs evoked in spinal cord slices were inhibited in a dose-dependent manner by the addition of GB to the superfusion solution.
The amplitude of eIPSCs was reduced to 61 ± 6.4% by 10 μM GB with acceleration of the kinetics of the currents, indicating
the effect of GB on channel pores. Both the amplitude and success ratio (Rsuc) of eIPSC induced by electrical focal stimulation of single glycinergic nerve endings (boutons) also changed in the presence
of 1 μM GB. These data suggest that GB modulates not only post-synaptic glycine receptors but also the pre-synaptic glycine
release machinery. 相似文献
702.
Summary Rates of human red blood cell hemolysis were measured as a function of temperature. Three distinct temperature intervals for hemolysis were noted: a) At temperatures equal to or less than 37°C no hemolysis was observed for the duration of the incubation (30 hr). b) For temperatures exceeding 45°C hemolysis rates are rapid and are accompanied by gross changes in cellular morphology. The activation energy for hemolysis is 80 kcal/mole; this value is characteristic of protein denaturation and enzyme inactivation suggesting that these processes contribute to hemolysis at these high temperatures. c) Between 38 and 45°C the energy of activation is 29 kcal/mole, indicating that a fundamentally different process than protein inactivation is responsible for hemolysis at these relatively low temperatures. A mechanism based on the concept of the critical bilayer assembly temperature of cell membranes (N.L. Gershfeld,Biophys. J.
50:457–461, 1986) accounts for hemolysis at these relatively mild temperatures: The unilamellar state of the membrane is stable at 37°C, but is transformed to a multibilayer when the temperature is raised; hemolysis results because formation of the multibilayer requires exposing lipid-free areas of the erythrocyte surface. An analysis of the activation energy for hemolysis is presented that is consistent with the proposed unilamellar-multibilayer transformation. 相似文献
703.
704.
Teiko Yamashita Jun-ichiro Murayama Hideo Utsumi Akira Hamada 《Biochimica et Biophysica Acta (BBA)/General Subjects》1985,839(1):26-31
Dog glycophorin, the major sialoglycoprotein of dog erythrocyte membranes, contains either N-acetyl- or N-glycolylneuraminic acid, depending upon the strain of dog. Glycolipids also contained the same sialic acid as those found in glycophorin when both materials are prepared from erythrocyte membranes of individual dogs. The O-glycosidic oligosaccharides were released from glycophorin, prepared from individual dogs, by alkaline borohydride treatment, and purified by gel filtration and ion-exchange chromatography. The structures of the reduced oligosaccharides were determined by methylation analysis and gas-liquid chromatography-mass spectrometry. The O-glycosidic oligoscharides identified were one tetrasaccharide - Neu5Ac(2→3)Gal)1→3)[Neu5Ac(2→6)[GalNAcol - two trisaccharides - Neu5Ac(2→3)Gal1→3)GaINAcol and Gal(1→3)[Neu5Ac(2→6)]GalNAcol. 相似文献
705.
Y Murayama T Ishida K Hashiba A Noguchi O Takenaka 《Journal of medical primatology》1989,18(2):111-123
Six mouse monoclonal antibodies were developed by immunization with a Japanese monkey cell line. These monoclonal antibodies, designated the UH series, reacted with large populations of peripheral B cells and monocytes, but not with T cells. The distribution of reactivities and the molecular weight of the membrane antigens recognized were similar to those of the HLA-DR monoclonal antibody; one inhibited the binding of HLA-DR. Human interferon-gamma induced increased expressions of all the UH antigen epitopes. 相似文献
706.
The short-term response of green pea stem segments to indole-3-aceticacid (IAA) was investigated by continuously recording stem elongationwith a differential transformer. Stem segment elongation promotedby IAA began following a latent period after application. Thelatent period was more effectively shortened by raising thetemperature rather than the concentration of IAA; it was reducednearly to 0 min by treatment at 40?C. The length of the latentperiod was not affected by turgor pressures of stem cells, thoughthe rate of stem growth was diminished at lower turgor pressures.Stems pretreated with actinomycin D for 60 min, cycloheximidefor 30 min or colchicin for 6 hr were similar to untreated stemsin their short term response to IAA. This implies that the initiallypromoted elongation does not result from the activity of enzymessynthesized during the latent period by the action of IAA. (Received April 5, 1973; ) 相似文献
707.
The expression of the epitope detected by the monoclonal antibody Leu-5 and the rosette formation with sheep red blood cells was examined for the mononuclear cells of 25 primate species including human subject. The plot of rosette-forming cells against Leu-5-positive cells enabled us to classify these species into three large groups: hominoids--Old World monkeys (Leu-5-positive cells greater than or equal to rosette-forming cells), New World monkeys (Leu-5-positive cells less than rosette-forming cells), and prosimians (Leu-5-positive cells less than 5%, some of them with both Leu-5-positive cells and rosette-forming cells less than 5%). 相似文献