全文获取类型
收费全文 | 5104篇 |
免费 | 377篇 |
国内免费 | 40篇 |
出版年
2024年 | 13篇 |
2023年 | 27篇 |
2022年 | 91篇 |
2021年 | 133篇 |
2020年 | 82篇 |
2019年 | 118篇 |
2018年 | 148篇 |
2017年 | 128篇 |
2016年 | 190篇 |
2015年 | 280篇 |
2014年 | 325篇 |
2013年 | 354篇 |
2012年 | 450篇 |
2011年 | 431篇 |
2010年 | 232篇 |
2009年 | 200篇 |
2008年 | 307篇 |
2007年 | 278篇 |
2006年 | 215篇 |
2005年 | 211篇 |
2004年 | 235篇 |
2003年 | 174篇 |
2002年 | 156篇 |
2001年 | 116篇 |
2000年 | 106篇 |
1999年 | 71篇 |
1998年 | 37篇 |
1997年 | 25篇 |
1996年 | 21篇 |
1995年 | 14篇 |
1994年 | 23篇 |
1993年 | 18篇 |
1992年 | 39篇 |
1991年 | 31篇 |
1990年 | 30篇 |
1989年 | 24篇 |
1988年 | 23篇 |
1987年 | 17篇 |
1986年 | 15篇 |
1985年 | 11篇 |
1984年 | 9篇 |
1983年 | 15篇 |
1981年 | 8篇 |
1979年 | 9篇 |
1978年 | 7篇 |
1976年 | 7篇 |
1975年 | 7篇 |
1973年 | 8篇 |
1972年 | 8篇 |
1971年 | 8篇 |
排序方式: 共有5521条查询结果,搜索用时 15 毫秒
81.
Jae-Jin Lee Hyun Ji Lee Gi Seon Jang Ja Myoung Yu Ji Yoon Cha Su Jeong Kim Eun Bit Lee Myung Kyum Kim 《Journal of microbiology (Seoul, Korea)》2013,51(3):305-311
Strain DY59T, a Gram-positive non-motile bacterium, was isolated from soil in South Korea, and was characterized to determine its taxonomic position. Phylogenetic analysis based on the 16S rRNA gene sequence of strain DY59T revealed that the strain DY59T belonged to the family Deinococcaceae in the class Deinococci. The highest degree of sequence similarities of strain DY59T were found with Deinococcus radiopugnans KACC 11999T (99.0%), Deinococcus marmoris KACC 12218T (97.9%), Deinococcus saxicola KACC 12240T (97.0%), Deinococcus aerolatus KACC 12745T (96.2%), and Deinococcus frigens KACC 12220T (96.1%). Chemotaxonomic data revealed that the predominant fatty acids were iso-C15:0 (19.0%), C16:1 ω7c (17.7%), C15:1 ω6c (12.6%), iso-C17:0 (10.3%), and iso-C17:1 ω9c (10.3%). A complex polar lipid profile consisted of a major unknown phosphoglycolipid. The predominant respiratory quinone is MK-8. The cell wall peptidoglycan contained D-alanine, L-glutamic acid, glycine, and L-ornithine (di-amino acid). The novel strain showed resistance to gamma radiation, with a D10 value (i.e. the dose required to reduce the bacterial population by 10-fold) in excess of 5 kGy. Based on the phylogenetic, chemotaxonomic, and phenotypic data, strain DY59T (=KCTC 33033T =JCM 18581T) should be classified as a type strain of a novel species, for which the name Deinococcus swuensis sp. nov. is proposed. 相似文献
82.
Byoung-Shik Shim Jung-ah Choi Ho-Hyun Song Sung-Moo Park In Su Cheon Ji-Eun Jang Sun Je Woo Chung Hwan Cho Min-Suk Song Hyemi Kim Kyung Joo Song Jae Myun Lee Suhng Wook Kim Dae Sub Song Young Ki Choi Jae-Ouk Kim Huan Huu Nguyen Dong Wook Kim Young Yil Bahk Cheol-Heui Yun Man Ki Song 《Journal of microbiology (Seoul, Korea)》2013,51(1):130-135
Influenza viruses are respiratory pathogens that continue to pose a significantly high risk of morbidity and mortality of humans worldwide. Vaccination is one of the most effective strategies for minimizing damages by influenza outbreaks. In addition, rapid development and production of efficient vaccine with convenient administration is required in case of influenza pandemic. In this study, we generated recombinant influenza virus hemagglutinin protein 1 (sHA1) of 2009 pandemic influenza virus as a vaccine candidate using a well-established bacterial expression system and administered it into mice via sublingual (s.l.) route. We found that s.l. immunization with the recombinant sHA1 plus cholera toxin (CT) induced mucosal antibodies as well as systemic antibodies including neutralizing Abs and provided complete protection against infection with pandemic influenza virus A/CA/04/09 (H1N1) in mice. Indeed, the protection efficacy was comparable with that induced by intramuscular (i.m.) immunization route utilized as general administration route of influenza vaccine. These results suggest that s.l. vaccination with the recombinant non-glycosylated HA1 protein offers an alternative strategy to control influenza outbreaks including pandemics. 相似文献
83.
Yochan Joung Haneul Kim Beom-Il Lee Heeyoung Kang Tae Yong Jang O-Seob Kwon Kiseong Joh 《Journal of microbiology (Seoul, Korea)》2013,51(4):531-535
A Gram-staining-negative, rod-shaped and red-pigmented bacterial strain, HMD3125T, was isolated from a solar saltern in Jeungdo, Republic of Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD3125T formed a lineage within the genus Pontibacter and was similar to Pontibacter salisaro (96.1%) and P. korlensis (95.3%). The major fatty acids of strain HMD3125T were summed feature 4 (comprising iso-C17:1 I and/or anteiso-C17:1 B; 30.4%), iso-C15:0 (20.4%) and iso-C17:0 3OH (17.2%). The polar lipid profile of HMD3125T consisted of the phosphatidylethanolamine, four unidentified polar lipids, unidentified phospholipid, unidentified aminolipid and unidentified aminophospholipid. Strain HMD3125T contained MK-7 as the predominant menaquinone and sym-homospermidine as the major polyamine. The DNA G+C content of strain HMD3125T was 45.6 mol%. Strain HMD3125T assigned as a novel species in the genus Pontibacter, for which the name Pontibacter jeungdoensis sp. nov. is proposed. The type strain is HMD3125T (=KCTC 23156T =CECT 7710T). 相似文献
84.
Seung Hyun Kim Sin-Duk Jang Ki Yong Lee Sang Hyun Sung 《Journal of enzyme inhibition and medicinal chemistry》2013,28(1):230-233
A methanolic extract of dried leaves of Polygala japonica Houtt (Polygalaceae) significantly attenuated nitric oxide production in lipopolysaccharide-simulated BV2 microglia. Five anthraquinones chrysophanol (1), emodin (2), aloe-emodin (3), emodin 8-O-β-D-glucopyranoside (4) and trihydroxy anthraquinone (5), and four flavonoids kaempferol (6), chrysoeriol (7), kaempferol 3-gentiobioside (8) and isorhamnetin (9) were isolated from the methanolic extract using bioactivity-guided fractionation. Among them, compounds 1–4, 6 and 7 showed significant inhibitory effect on lipopolysaccharide-induced nitric oxide production in BV2 microglia at the concentrations ranging from 1.0 to 100.0 μM. 相似文献
85.
86.
87.
Wen-Chih Wu Chih-Hsiung Hsu Jen-Chun Kuan Jih-Fu Hsieh Chien-An Sun Tsan Yang Chang-Chieh Wu Yu-Ching Chou 《Genetics and molecular biology》2013,36(3):323-328
A new noninvasive screening tool for colorectal neoplasia detects epigenetic alterations exhibited by gastrointestinal tumor cells shed into stool. There is insufficient existing data to determine temporal associations between colorectal cancer (CRC) progression and aberrant DNA methylation. To evaluate the feasibility of using fecal DNA methylation status to determine CRC progression, we collected stool samples from 14 male SD rats aged six weeks, and administered subcutaneous injections of either 1,2-dimethylhydrazine or saline weekly. p16 DNA methylation statuses in tumorous and normal colon tissue, and from stool samples were determined using methylation-specific PCR. Additionally, p16 methylation was detected in stool DNA from 85.7% of the CRC rats. The earliest change in p16 methylation status in the DMH-treated group stool samples occurred during week nine; repeatabilities were 57.1% in week 19 (p = 0.070) and 85.7% in week 34 (p = 0.005). A temporal correlation was evidenced between progression of CRC and p16 methylation status, as evidenced by DMH-induced rat feces. Using fecal DNA methylation status to determine colorectal tissue methylation status can reveal CRC progression. Our data suggests that p16 promoter methylation is a feasible epigenetic marker for the detection and may be useful for CRC screening. 相似文献
88.
Jong-Kuen Lee Sae-Ra Park Bong-Kwang Jung Yoon-Kyung Jeon Yeong-Shin Lee Min-Kyoung Kim Yong-Goo Kim Ji-Young Jang Chul-Woo Kim 《PloS one》2013,8(12)
Exosomes are small membrane vesicles released by a variety of cell types. Exosomes contain genetic materials, such as mRNAs and microRNAs (miRNAs), implying that they may play a pivotal role in cell-to-cell communication. Mesenchymal stem cells (MSCs), which potentially differentiate into multiple cell types, can migrate to the tumor sites and have been reported to exert complex effects on tumor progression. To elucidate the role of MSCs within the tumor microenvironment, previous studies have suggested various mechanisms such as immune modulation and secreted factors of MSCs. However, the paracrine effects of MSC-derived exosomes on the tumor microenvironment remain to be explored. The hypothesis of this study was that MSC-derived exosomes might reprogram tumor behavior by transferring their molecular contents. To test this hypothesis, exosomes from MSCs were isolated and characterized. MSC-derived exosomes exhibited different protein and RNA profiles compared with their donor cells and these vesicles could be internalized by breast cancer cells. The results demonstrated that MSC-derived exosomes significantly down-regulated the expression of vascular endothelial growth factor (VEGF) in tumor cells, which lead to inhibition of angiogenesis in vitro and in vivo. Additionally, miR-16, a miRNA known to target VEGF, was enriched in MSC-derived exosomes and it was partially responsible for the anti-angiogenic effect of MSC-derived exosomes. The collective results suggest that MSC-derived exosomes may serve as a significant mediator of cell-to-cell communication within the tumor microenvironment and suppress angiogenesis by transferring anti-angiogenic molecules. 相似文献
89.
90.
Mi-Hyeon Jang Michael A. Bonaguidi Yasuji Kitabatake Jiaqi Sun Juan Song Eunchai Kang Heechul Jun Chun Zhong Yijing Su Junjie U. Guo Marie Xun Wang Kurt A. Sailor Ju-Young Kim Yuan Gao Kimberly M. Christian Guo-li Ming Hongjun Song 《Cell Stem Cell》2013,12(2):215-223
Highlights? Wnt inhibitor sFRP3 exhibits activity-dependent expression in the adult hippocampus ? sFRP3 maintains quiescence of adult hippocampal radial glia-like neural stem cells ? sFRP3 inhibits maturation, dendritic development, and spinal formation of new neurons ? sFRP3 partially mediates activity-dependent adult hippocampal neurogenesis 相似文献