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101.
Suppression of gene expression by small interfering RNA (siRNA) has proved to be a gene-specific and cost effective alternative
to other gene suppression technologies. Short hairpin RNAs (shRNAs) generated from the vector-based expression are believed
to be processed into functional siRNAs in vivo, leading to gene silencing. Since an shRNA library carries a large pool of potential siRNAs, such a library makes it possible
to knock down gene expression at the genome wide scale. Although much of research has been focused on generating shRNA libraries
from either individually made gene specific sequences or cDNA libraries, there is no report on constructing randomized shRNA
libraries, which could provide a good alternative to these existing libraries. We have developed a method of constructing
shRNAs from randomized oligonucleotides. Through this method, one can generate a partially or fully randomized shRNA library
for various functional analyses. We validated this procedure by constructing a p53-specific shRNA. Western blot revealed that
the p53-shRNA successfully suppressed expression of the endogenous p53 in MCF-7 cells. We then made a partially randomized
shRNA library. Sequencing of 15 randomly picked cloned confirmed the randomness of the library. Therefore, the library can
be used for various functional assays, such as target validation when a suitable screening or selection method is available. 相似文献
102.
Binh Tran Quang Linh Duong Tuan Chung Le Thi Kim Phuong Pham Tran Nga Bui Thi Thuy Ngoc Nguyen Anh Thuyen Tran Quang Tung Do Dinh Nhung Bui Thi 《Biochemical genetics》2022,60(2):707-719
Biochemical Genetics - The study aimed to evaluate the contribution of the FTO A/T polymorphism (rs9939609) to the prediction of the future type 2 diabetes (T2D). A population-based prospective... 相似文献
103.
Nguyen Anh T. Q. Nguyen Anh M. Nguyen Muu T. Nguyen Hue T. Duong Lim T. Dinh Van M. Nguyen Phuong M. Dultz Stefan Nguyen Minh N. 《Biogeochemistry》2022,160(1):35-47
Biogeochemistry - Phytolith is widely known as a silica structure in numerous silicon (Si) accumulator plants, e.g., rice, and it contains various nutrients and other beneficial elements. When rice... 相似文献
104.
Jiansong Xu Stephen M. Healy Dennis Q. Truong Abhishek Datta Marom Bikson Marc N. Potenza 《The Yale journal of biology and medicine》2015,88(3):219-225
Transcranial direct current stimulation (tDCS) studies often use one anode to increase cortical excitability in one hemisphere. However, mental processes may involve cortical regions in both hemispheres. This study’s aim was to assess the safety and possible effects on affect and working memory of tDCS using two anodes for bifrontal stimulation. A group of healthy subjects participated in two bifrontal tDCS sessions on two different days, one for real and the other for sham stimulation. They performed a working memory task and reported their affect immediately before and after each tDCS session. Relative to sham, real bifrontal stimulation did not induce significant adverse effects, reduced decrement in vigor-activity during the study session, and did not improve working memory. These preliminary findings suggest that bifrontal anodal stimulation is feasible and safe and may reduce task-related fatigue in healthy participants. Its effects on neuropsychiatric patients deserve further study. 相似文献
105.
Truong Quoc Phong Do Thi Thu Ha Uwe Volker Elke Hammer 《Indian journal of microbiology》2015,55(2):219-230
Reports in recent years indicate that the increasing emergence of resistance to drugs be using to TB treatment. The resistance to them severely affects to options for effective treatment. The emergence of multidrug-resistant tuberculosis has increased interest in understanding the mechanism of drug resistance in M. tuberculosis and the development of new therapeutics, diagnostics and vaccines. In this study, a label-free quantitative proteomics approach has been used to analyze proteome of multidrug-resistant and susceptible clinical isolates of M. tuberculosis and identify differences in protein abundance between the two groups. With this approach, we were able to identify a total of 1,583 proteins. The majority of identified proteins have predicted roles in lipid metabolism, intermediary metabolism, cell wall and cell processes. Comparative analysis revealed that 68 proteins identified by at least two peptides showed significant differences of at least twofolds in relative abundance between two groups. In all protein differences, the increase of some considering proteins such as NADH dehydrogenase, probable aldehyde dehydrogenase, cyclopropane mycolic acid synthase 3, probable arabinosyltransferase A, putative lipoprotein, uncharacterized oxidoreductase and six membrane proteins in resistant isolates might be involved in the drug resistance and to be potential diagnostic protein targets. The decrease in abundance of proteins related to secretion system and immunogenicity (ESAT-6-like proteins, ESX-1 secretion system associated proteins, O-antigen export system and MPT63) in the multidrug-resistant strains can be a defensive mechanism undertaken by the resistant cell.
Electronic supplementary material
The online version of this article (doi:10.1007/s12088-015-0511-2) contains supplementary material, which is available to authorized users. 相似文献106.
107.
Jean-Pierre Dujardin Truong Xuan Lam Pham Thi Khoa Christopher John Schofield 《Memórias do Instituto Oswaldo Cruz》2015,110(3):319-323
The migration of invasive vector species has contributed to the worldwide extension
of infectious diseases such as dengue (Aedes aegypti) and chikungunya (Aedes
albopictus). It is probably a similar behaviour for certain vectors of Chagas disease
which allowed it to become a continental burden in Latin America. One of them,
Triatoma rubrofasciata has also been spreading throughout the tropical and
subtropical world. Here, the recent and massive peridomestic presence of T.
rubrofasciata in Vietnam cities is reported, and tentatively explained, highlighting
the need for improved entomological surveillance. 相似文献
108.
Chuping Luo Xuehui Liu Xian Zhou Junyao Guo John Truong Xiaoyu Wang Huafei Zhou Xiangqian Li Zhiyi Chen 《Applied and environmental microbiology》2015,81(19):6601-6609
Three families of Bacillus cyclic lipopeptides—surfactins, iturins, and fengycins—have well-recognized potential uses in biotechnology and biopharmaceutical applications. This study outlines the isolation and characterization of locillomycins, a novel family of cyclic lipopeptides produced by Bacillus subtilis 916. Elucidation of the locillomycin structure revealed several molecular features not observed in other Bacillus lipopeptides, including a unique nonapeptide sequence and macrocyclization. Locillomycins are active against bacteria and viruses. Biochemical analysis and gene deletion studies have supported the assignment of a 38-kb gene cluster as the locillomycin biosynthetic gene cluster. Interestingly, this gene cluster encodes 4 proteins (LocA, LocB, LocC, and LocD) that form a hexamodular nonribosomal peptide synthetase to biosynthesize cyclic nonapeptides. Genome analysis and the chemical structures of the end products indicated that the biosynthetic pathway exhibits two distinct features: (i) a nonlinear hexamodular assembly line, with three modules in the middle utilized twice and the first and last two modules used only once and (ii) several domains that are skipped or optionally selected. 相似文献
109.
110.
Isolation of New Delhi metallo‐β‐lactamase 1‐producing Vibrio cholerae non‐O1, non‐O139 strain carrying ctxA,st and hly genes in southern Vietnam 下载免费PDF全文
Tai The Diep Nhi Thi Ngoc Nguyen Thi Ngoc Cat Nguyen Huy Khac An Truong Quang Nguyen Vu Hoang Nguyen Thuong Van Nguyen Thu Ngoc Anh Nguyen Hidemasa Izumiya Makoto Ohnishi Tetsu Yamashiro Lan Thi Phuong Nguyen 《Microbiology and immunology》2015,59(5):262-267
Vibrio cholerae non‐O1, non‐O139 (VC_NAG) organisms are universally present in the aquatic environment and regarded as non‐pathogenic bacteria. However, considering that they do occasionally induce gastroenteritis, a study of their virulence and antibiotic resistance genes is important. The presence of enteropathogenic genes, including ctxA, VC_NAG‐specific heat‐stable toxin gene (st), hemolysin (hly), and zona occludens toxin (zot) was determined by PCR in 100 VC_NAG strains isolated in southern Vietnam in 2010–2013 from 94 environmental and six human origins. These 100 VC_NAG strains were also tested phenotypically and genotypically for the presence of the New Delhi metallo‐β‐lactamase (NDM‐1). Of the 100 VC_NAG strains tested, six were positive for ctxA; five from the environment and one of human origin. The st gene was detected in 17 isolates, 15 and two of which were of environmental and human origins, respectively. Gene hly was detected in 19 VC_NAG strains examined, two of which were isolated from humans and 17 from environments. The zot gene was not detected in any of the strains tested. Three VC_NAG strains of environmental origin were confirmed to produce NDM‐1 and the blaNDM‐1 gene was detected in those strains by PCR. Of note, one of the three NDM‐1‐producing VC_NAG strains was confirmed to carry ctxA, st and hly genes concurrently. This is the first report of isolation of NDM‐1‐producing VC_NAG strains in Vietnam. 相似文献