全文获取类型
收费全文 | 599篇 |
免费 | 35篇 |
国内免费 | 2篇 |
出版年
2023年 | 5篇 |
2022年 | 10篇 |
2021年 | 35篇 |
2020年 | 16篇 |
2019年 | 33篇 |
2018年 | 16篇 |
2017年 | 14篇 |
2016年 | 17篇 |
2015年 | 52篇 |
2014年 | 38篇 |
2013年 | 47篇 |
2012年 | 47篇 |
2011年 | 67篇 |
2010年 | 24篇 |
2009年 | 18篇 |
2008年 | 30篇 |
2007年 | 30篇 |
2006年 | 21篇 |
2005年 | 23篇 |
2004年 | 18篇 |
2003年 | 15篇 |
2002年 | 15篇 |
2001年 | 6篇 |
2000年 | 3篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 5篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1984年 | 2篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 1篇 |
1858年 | 1篇 |
排序方式: 共有636条查询结果,搜索用时 62 毫秒
481.
David J. P. Moore Nicole A. Trahan Phil Wilkes Tristan Quaife Britton B. Stephens Kelly Elder Ankur R. Desai Jose Negron Russell K. Monson 《Ecology letters》2013,16(6):731-737
Amid a worldwide increase in tree mortality, mountain pine beetles (Dendroctonus ponderosae Hopkins) have led to the death of billions of trees from Mexico to Alaska since 2000. This is predicted to have important carbon, water and energy balance feedbacks on the Earth system. Counter to current projections, we show that on a decadal scale, tree mortality causes no increase in ecosystem respiration from scales of several square metres up to an 84 km2 valley. Rather, we found comparable declines in both gross primary productivity and respiration suggesting little change in net flux, with a transitory recovery of respiration 6–7 years after mortality associated with increased incorporation of leaf litter C into soil organic matter, followed by further decline in years 8–10. The mechanism of the impact of tree mortality caused by these biotic disturbances is consistent with reduced input rather than increased output of carbon. 相似文献
482.
Matthew J. Smukall Steven T. Kessel Bryan R. Franks Kevin A. Feldheim Tristan L. Guttridge Samuel H. Gruber 《Journal of fish biology》2019,94(1):173-177
An intact and uncompromised internal acoustic transmitter was non-lethally recovered from a lemon shark Negaprion brevirostris, after 13 years at liberty. The shark, first tagged at an estimated age of 2 years old near South Bimini, Bahamas in 2004, was recaptured in 2017 with a total length of 264 cm. The tagged shark displayed typical growth rate, pregnancy, natal homing and pupping behaviour of other individuals in this population. This observation provides important evidence regarding the effects from long-term retention of implanted acoustic transmitters in a carcharhinid shark. 相似文献
483.
Jonathan Huc Katarzyna Dziasek Kannan Pachamuthu Tristan Woh Claudia Khler Filipe Borges 《The Plant cell》2022,34(3):989
The triploid block, which prevents interploidy hybridizations in flowering plants, is characterized by a failure in endosperm development, arrest in embryogenesis, and seed collapse. Many genetic components of triploid seed lethality have been successfully identified in the model plant Arabidopsis thaliana, most notably the paternally expressed genes (PEGs), which are upregulated in tetraploid endosperm with paternal excess. Previous studies have shown that the paternal epigenome is a key determinant of the triploid block response, as the loss of DNA methylation in diploid pollen suppresses the triploid block almost completely. Here, we demonstrate that triploid seed collapse is bypassed in Arabidopsis plants treated with the DNA methyltransferase inhibitor 5-Azacytidine during seed germination and early growth. We identified strong suppressor lines showing stable transgenerational inheritance of hypomethylation in the CG context, as well as normalized expression of PEGs in triploid seeds. Importantly, differentially methylated loci segregate in the progeny of “epimutagenized” plants, which may allow epialleles involved in the triploid block response to be identified in future studies. Finally, we demonstrate that chemically induced epimutagenesis facilitates hybridization between different Capsella species, thus potentially emerging as a strategy for producing triploids and interspecific hybrids with high agronomic interest.Genome-wide loss of DNA methylation induced by 5-Azacytidine allows interploidy and interspecific hybridization barriers to be bypassed in Arabidopsis and Capsella. 相似文献
484.
Since their introduction in the 50’s, variance component mixed models have been widely used in many application fields. In this context, ReML estimation is by far the most popular procedure to infer the variance components of the model. Although many implementations of the ReML procedure are readily available, there is still need for computational improvements due to the ever-increasing size of the datasets to be handled, and to the complexity of the models to be adjusted. In this paper, we present a Min-Max (MM) algorithm for ReML inference and combine it with several speed-up procedures. The ReML MM algorithm we present is compared to 5 state-of-the-art publicly available algorithms used in statistical genetics. The computational performance of the different algorithms are evaluated on several datasets representing different plant breeding experimental designs. The MM algorithm ranks among the top 2 methods in almost all settings and is more versatile than many of its competitors. The MM algorithm is a promising alternative to the classical AI-ReML algorithm in the context of variance component mixed models. It is available in the MM4LMM R-package. 相似文献
485.
Srivas Chennu Paola Finoia Evelyn Kamau Judith Allanson Guy B. Williams Martin M. Monti Valdas Noreika Aurina Arnatkeviciute Andrés Canales-Johnson Francisco Olivares Daniela Cabezas-Soto David K. Menon John D. Pickard Adrian M. Owen Tristan A. Bekinschtein 《PLoS computational biology》2014,10(10)
Theoretical advances in the science of consciousness have proposed that it is concomitant with balanced cortical integration and differentiation, enabled by efficient networks of information transfer across multiple scales. Here, we apply graph theory to compare key signatures of such networks in high-density electroencephalographic data from 32 patients with chronic disorders of consciousness, against normative data from healthy controls. Based on connectivity within canonical frequency bands, we found that patient networks had reduced local and global efficiency, and fewer hubs in the alpha band. We devised a novel topographical metric, termed modular span, which showed that the alpha network modules in patients were also spatially circumscribed, lacking the structured long-distance interactions commonly observed in the healthy controls. Importantly however, these differences between graph-theoretic metrics were partially reversed in delta and theta band networks, which were also significantly more similar to each other in patients than controls. Going further, we found that metrics of alpha network efficiency also correlated with the degree of behavioural awareness. Intriguingly, some patients in behaviourally unresponsive vegetative states who demonstrated evidence of covert awareness with functional neuroimaging stood out from this trend: they had alpha networks that were remarkably well preserved and similar to those observed in the controls. Taken together, our findings inform current understanding of disorders of consciousness by highlighting the distinctive brain networks that characterise them. In the significant minority of vegetative patients who follow commands in neuroimaging tests, they point to putative network mechanisms that could support cognitive function and consciousness despite profound behavioural impairment. 相似文献
486.
Daniel Cabaret Sophie Bourcier Sophie Maillot Michel Wakselman 《Biocatalysis and Biotransformation》1992,6(3):191-199
-Chymotrypsin has been modified by a series of neutral liposaccharidic or charged lipocarboxylic amphiphile reagents. In the esterification of N-acetyl tyrosine in three polar solvents, the new biocatalysts have been compared to chymotrypsins modified by reductive alkylation with glyoxylic acid, melibiose or octanal. This comparison indicates that the rate accelerations observed with the neutral or anionic amphiphile-coated enzymes are mainly due to the hydrophobization of the protein surface in the neigbourhood of the external lysine residues. This interpretation is strengthened by the favorable effect of supports more hydrophobic than celite on the reaction. 相似文献
487.
488.
Tristan Biard 《Environmental microbiology》2022,24(5):2179-2200
Among the many inhabitants of planktonic communities, several lineages have biomineralized intricate skeletons. These have existed for millions of years and include the Radiolaria, a group of marine protists, many of which bear delicate mineral skeletons of different natures. Radiolaria are well known for their paleontological signatures, but little is known about the ecology of modern assemblages. They are found from polar to tropical regions, in the sunlit layers of the ocean down to the deep and cold bathypelagic. They are closely involved in the biogeochemical cycles of silica, carbon and strontium sulfate, carrying important amounts of such elements to the deep ocean. However, relatively little is known on the actual extent of genetic diversity or biogeographic patterns. The rapid emergence and acceptance of molecular approaches have nevertheless led to major advances in our understanding of diversity within and evolutionary relationships between major radiolarian groups. Here, we review the state of knowledge relating to the classification, diversity and ecology of extant radiolarian orders, highlighting the substantial gaps in our understanding of the extent of their contribution to marine biodiversity and their role in marine food webs. 相似文献
489.
490.