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Trine Glad Pål Bernhardsen Kaare M Nielsen Lorenzo Brusetti Magnus Andersen Jon Aars Monica A Sundset 《BMC microbiology》2010,10(1):10
Background
Polar bears (Ursus maritimus) are major predators in the Arctic marine ecosystem, feeding mainly on seals, and living closely associated with sea ice. Little is known of their gut microbial ecology and the main purpose of this study was to investigate the microbial diversity in faeces of polar bears in Svalbard, Norway (74-81°N, 10-33°E). In addition the level of bla TEM alleles, encoding ampicillin resistance (ampr) were determined. In total, ten samples were collected from ten individual bears, rectum swabs from five individuals in 2004 and faeces samples from five individuals in 2006. 相似文献64.
Plants of the fern Matteuccia struthiopteris from northern and southern populations in Norway were studied in a phytotron. Relative growth rate (RGR), growth period, and sporophyll production were measured under different photoperiod (12–24 h) day-length and temperature (9–21 °C) treatments. For the southern plants, there were no significant differences between the different light treatments, but for the northern plants there was a significant (p<0.01) linear increase in the mean maximum RGR with increasing day-length. Small, but statistically significant (p<0.01) differences were found between northern and southern plants when the mean maximum RGR-values were compared. When plants from different origin were exposed to different treatment, there were major differences between the populations in the production of sporophylls (p<0.0001). Southern plants produced in average three times more sporophylls than the northern plants, and they had also higher proportions of fertile plants. Diurnal alternating temperature treatments gave no significant (p>0.05) effect on the mean maximum RGR compared with constant temperatures, but they gave significantly higher production of sporophylls. In general, the northern plants had a higher temperature threshold (approximately 12 °C) for sporophyll production than the southern plants (approximately 9 °C). Plants exposed to 24 h with natural light were generally more often fertile than plants exposed to a shorter photoperiod. The mean maximum RGR-values and time needed to develop the fronds at the 9 °C treatment were fairly equal to those found under natural conditions close to the altitudinal distribution limit of M.struthiopteris in W Norway. In general, the investigation showed that the applied temperature and light treatments affected sporophyll production more than vegetative growth. 相似文献
65.
Diversity of sulfate-reducing bacteria from an extreme hypersaline sediment, Great Salt Lake (Utah) 总被引:2,自引:0,他引:2
Kjeldsen KU Loy A Jakobsen TF Thomsen TR Wagner M Ingvorsen K 《FEMS microbiology ecology》2007,60(2):287-298
The diversity of sulfate-reducing bacteria (SRB) inhabiting the extreme hypersaline sediment (270 g L(-1) NaCl) of the northern arm of Great Salt Lake was studied by integrating cultivation and genotypic identification approaches involving PCR-based retrieval of 16S rRNA and dsrAB genes, the latter encoding major subunits of dissimilatory (bi) sulfite reductase. The majority (85%) of dsrAB sequences retrieved directly from the sediment formed a lineage of high (micro) diversity affiliated with the genus Desulfohalobium, while others represented novel lineages within the families Desulfohalobiaceae and Desulfobacteraceae or among Gram-positive SRB. Using the same sediment, SRB enrichment cultures were established in parallel at 100 and at 190 g L(-1) NaCl using different electron donors. After 5-6 transfers, dsrAB and 16S rRNA gene-based profiling of these enrichment cultures recovered a SRB community composition congruent with the cultivation-independent profiling of the sediment. Pure culture representatives of the predominant Desulfohalobium-related lineage and of one of the Desulfobacteraceae-affilated lineages were successfully obtained. The growth performance of these isolates and of the enrichment cultures suggests that the sediment SRB community of the northern arm of Great Salt Lake consists of moderate halophiles, which are salt-stressed at the in situ salinity of 27%. 相似文献
66.
The abundance of potential denitrifiers in full-scale wastewater treatment plants with biological nitrogen and phosphorus removal was investigated by FISH and various oligonucleotide probes. The potential denitrifiers were characterized as probe-defined populations that were able to consume radiolabelled substrate with oxygen, nitrate and nitrite as electron acceptor as determined by microautoradiography. The most abundant potential denitrifiers were related to the genera Aquaspirillum, Azoarcus, Thauera and Rhodocyclus, all within the Betaproteobacteria. They made up 20-49% of all bacteria in most of the 17 nitrogen removal plants investigated and were hardly present in four plants without denitrification. The ecophysiology of Aquaspirillum, Azoarcus and Thauera-related bacteria was consistent within each probe-defined group in the plants investigated. These three groups showed distinct physiological differences, with the Aquaspirillum-related bacteria appearing as the most specialized one, consuming only amino acids among the substrates tested, and Thauera as the most versatile consuming some volatile fatty acids, ethanol and amino acids. The coexistence of Aquaspirillum, Azoarcus and Thauera-related bacteria in a range of treatment plants with differences in wastewater, design and operation suggest that the populations ensure a functional stability of the plants by occupying different ecological niches related to the carbon transformation. 相似文献
67.
Jorgensen TN McKee A Wang M Kushnir E White J Refaeli Y Kappler JW Marrack P 《Journal of immunology (Baltimore, Md. : 1950)》2007,179(6):3417-3424
The life and death of T cells is controlled to a large extent by the relative amounts of Bcl-2-related proteins they contain. The antiapoptotic protein Bcl-2 and the proapoptotic protein Bim are particularly important in this process with the amount of Bcl-2 per cell dropping by about one-half when T cells prepare to die. In this study we show that Bcl-2 and Bim each control the expression of the other. Absence of Bim leads to a drop in the amount of intracellular Bcl-2 protein, while having no effect on the amounts of mRNA for Bcl-2. Conversely, high amounts of Bcl-2 per cell allow high amounts of Bim, although in this case the effect involves increases in Bim mRNA. These mutual effects occur even if Bcl-2 is induced acutely. Thus these two proteins control the expression of the other, at either the protein or mRNA level. 相似文献
68.
Changes in brain levels of N-acylethanolamines and 2-arachidonoylglycerol in focal cerebral ischemia in mice 总被引:1,自引:0,他引:1
Degn M Lambertsen KL Petersen G Meldgaard M Artmann A Clausen BH Hansen SH Finsen B Hansen HS Lund TM 《Journal of neurochemistry》2007,103(5):1907-1916
The N -acylethanolamines (NAEs) and 2-arachidonoylglycerol (2-AG) are bioactive lipids that can modulate inflammatory responses and protect neurons against glutamatergic excitotoxicity. We have used a model of focal cerebral ischemia in young adult mice to investigate the relationship between focal cerebral ischemia and endogenous NAEs. Over the first 24 h after induction of permanent middle cerebral artery occlusion, we observed a time-dependent increase in all the investigated NAEs, except for anandamide. Moreover, we found an accumulation of 2-AG at 4 h that returned to basal level 12 h after induction of ischemia. Accumulation of NAEs did not depend on regulation of N -acylphosphatidylethanolamine-hydrolyzing phospholipase D or fatty acid amide hydrolase. Treatment with the fatty acid amide hydrolase inhibitor URB597 (cyclohexyl carbamic acid 3'-carbamoyl-biphenyl-3-yl ester; 1 mg/kg; i.p.) 1.5 h before arterial occlusion decreased the infarct volume in our model system. Our results suggest that NAEs and 2-AG may be involved in regulation of neuroprotection during focal cerebral ischemia in mice. 相似文献
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Gorodkin J Cirera S Hedegaard J Gilchrist MJ Panitz F Jørgensen C Scheibye-Knudsen K Arvin T Lumholdt S Sawera M Green T Nielsen BJ Havgaard JH Rosenkilde C Wang J Li H Li R Liu B Hu S Dong W Li W Yu J Wang J Staefeldt HH Wernersson R Madsen LB Thomsen B Hornshøj H Bujie Z Wang X Wang X Bolund L Brunak S Yang H Bendixen C Fredholm M 《Genome biology》2007,8(4):R45-16